Results 41 to 50 of about 79,886 (234)

Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]

, 2015
Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas, LaFayette, Peter R, Parrott, Wayne A, Schmitz, Robert J   +3 more
core   +2 more sources

CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges

Heliyon
CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 and CRISPR/Cas3 utilize different mechanisms to detect or ...
Jian Zhou, Zhuo Li, Joshua Seun Olajide, Gang Wang   +3 more
doaj   +1 more source

Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations

eLife, 2017
CRISPR-Cas provides a barrier to horizontal gene transfer in prokaryotes. It was previously observed that functional CRISPR-Cas systems are absent from multidrug-resistant (MDR) Enterococcus faecalis, which only possess an orphan CRISPR locus, termed ...
Karthik Hullahalli, Marinelle Rodrigues, Kelli L Palmer   +2 more
doaj   +1 more source

Protospacer-Adjacent Motif Specificity during Clostridioides difficile Type I-B CRISPR-Cas Interference and Adaptation

mBio, 2021
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems provide prokaryotes with efficient protection against foreign nucleic acid invaders. We have recently demonstrated the defensive interference function of a
Anna Maikova, Pierre Boudry, Anna Shiriaeva, Aleksandra Vasileva, Anaïs Boutserin, Sofia Medvedeva, Ekaterina Semenova, Konstantin Severinov, Olga Soutourina   +8 more
doaj   +1 more source

How the other half lives: CRISPR-Cas's influence on bacteriophages

, 2017
CRISPR-Cas is a genetic adaptive immune system unique to prokaryotic cells used to combat phage and plasmid threats. The host cell adapts by incorporating DNA sequences from invading phages or plasmids into its CRISPR locus as spacers.
AE Briner, AF Andersson, BR Levin, C Pourcel, CL Sun, D Bhaya, E Pennisi, E Scarpellini, EV Koonin, FS Berezovskaya, GW Tyson, H Deveau, H Deveau, I Yosef, IN Berezovsky, J He, J Iranzo, J Mahony, JF Heidelberg, JM Peters, JO Haerter, KD Seed, KL Maxwell, LA Marraffini, LM Childs, M Sebaihia, P Han, P Horvath, P Horvath, PD Hsu, R Barrangou, R Lillestøl, R Sorek, S Al-Attar, S Minot, W Jiang   +35 more
core   +1 more source

Boosting plant immunity with CRISPR/Cas [PDF]

, 2015
CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method.See related Research: http://www.genomebiology.com/2015/16/1 ...
Chaparro-Garcia, Angela, Kamoun, Sophien, Nekrasov, Vladimir   +2 more
core   +1 more source

Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]

, 2018
The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan, Liu, Fenyong, Sheng, Jingxue, Trang, Phong   +3 more
core   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Correlation of CRISPR/Cas and Antimicrobial Resistance in Klebsiella pneumoniae Clinical Isolates Recovered from Patients in Egypt Compared to Global Strains

Microorganisms, 2023
The CRISPR/Cas system has been long known to interfere with the acquisition of foreign genetic elements and was recommended as a tool for fighting antimicrobial resistance.
Amany K. Alkompoz, Samira M. Hamed, Ahmed S. Abu Zaid, Thamer A. Almangour, Mohamed H. Al-Agamy, Khaled M. Aboshanab   +5 more
doaj   +1 more source

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source