Results 81 to 90 of about 1,216,467 (232)

CRISPR-Cas-Based Biomonitoring for Marine Environments: Toward CRISPR RNA Design Optimization Via Deep Learning

, 2023
Almost all of Earth's oceans are now impacted by multiple anthropogenic stressors, including the spread of nonindigenous species, harmful algal blooms, and pathogens. Early detection is critical to manage these stressors effectively and to protect marine
Durán-Vinet, Benjamín, Kardailsky, Anya, Susanna A. Wood, Neil J. Gemmell, Xavier Pochon, Maryam Moarefian, Jeunen, Gert-Jan, Anya Kardailsky, Aran, Kiana, Araya-Castro, Karla, Jo-Ann L. Stanton, Tzu-Chiao Chao, Anastasija Zaiko, Michelle Scriver, Deependra K. Ban, Benjamín Durán-Vinet, Ban, Deependra K, Wood, Susanna A, Pochon, Xavier, Gemmell, Neil J, Wood, Susanna, Moarefian, Maryam, Stanton, Jo-Ann L, Karla Araya-Castro, Kiana Aran, Gert-Jan Jeunen, Chao, Tzu-Chiao, Zaiko, Anastasija, Scriver, Michelle   +28 more
core   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

CRISPR-Cas Systems Impact Pseudomonas aeruginosa Genome Structure

, 2016
Pseudomonas aeruginosa is both an antibiotic-resistant opportunistic pathogen and an important model of type I clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR-Cas) systems.
Kristofer Miller (2862299), Kyle C. Cady (2862296), Todd C. Peterson (2427787), Gregory Hannum (255439), Toby H. Richardson (251546), Leah B. Soriaga (2862302), E. Magda Barbu (254713), Daniel Brami (126239), Srividya Akella (2862293), Bolyn Hubby (94177), Martina Felderman (2862305), Ariel Schwartz (521629), Matthew C. LaFave (594263), Alex van Belkum (38419), Mark C. Enright (255200)   +14 more
core   +1 more source

CRISPR: An overview of CRISPR-Cas systems and their diagnostic methods for pathogen detection

Life Sciences, Medicine and Biomedicine
Early and rapid diagnosis of pathogens is crucial for preventing and controlling epidemic diseases. While traditional microbial detection methods serve their purpose, they often have limitations such as low sensitivity and specificity.
Pui Yuei Lee, Christina Shook Cheng Chong, Mee Hoong See, Lee Lee Lai, Paul Michels, Choon Jiat Lee, Rukumani Devi Velayuthan, Yoke Kiet Doon, Yong Hui Tan, Yin Yin Lau, Sze Wan Poong, Phaik Eem Lim, Crystale Siew Ying Lim   +12 more
doaj   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system

Heliyon
CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications.
Hui Cheng, Haoyue Deng, Dongdao Ma, Mengyuan Gao, Zhihan Zhou, Heng Li, Shejuan Liu, Tieshan Teng   +7 more
doaj   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Complete Genome Sequence of Propionibacterium avidum Strain 44067, Isolated from a Human Skin Abscess [PDF]

, 2013
Propionibacterium avidum is an anaerobic Gram-positive bacterium that forms part of the normal human cutaneous microbiota, colonizing moist areas such as the vestibule of the nose, axilla, and perineum.
Kereszt, Attila, Szűcs, Attila, Kondorosi, Éva, Nagy, István, Hunyadkürti, Judit, Horváth, Balázs, Zsoldiné Urbán, Edit, Ördögh, Lilla, Vörös, Andrea   +8 more
core   +2 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source