Results 31 to 40 of about 1,254,871 (374)

CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]

, 2020
The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I, Jacinto, Filipe, Link, Wolfgang   +2 more
core   +1 more source

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana [PDF]

, 2016
Background: CRISPR-Cas is a recent and powerful addition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date.
A Scheffel, A Xiao, Amanda Hopes, B Delalat, BJ Karas, C Bowler, C Brooks, C Fan, C Jeffryes, C Krysiak, CB Field, CUT Hellen, E Jabri, E Weber, E Weber, ES Lander, EV Armbrust, F Daboussi, FL Pinto, G d’Ippolito, H-L Xing, JA Doudna, JD Sander, JE Habel, JEN Dolatabadi, JG Doench, K Belhaj, M Jinek, M Nymark, N Poulsen, N Poulsen, NM Price, O Cook, P Kuczynska, P Stothard, PD Weyman, PG Falkowski, Q Zheng, R Tarleton, RJ Olson, RP Shrestha, S Gupta, S Malviya, S-E Shin, Sophien Kamoun, T Sakuma, TB Jacobs, Thomas Mock, TTY Doan, V Nekrasov, V Smetacek, Vladimir Nekrasov, W Li, WHCF Kooistra   +53 more
core   +2 more sources

Associate toxin-antitoxin with CRISPR-Cas to kill multidrug-resistant pathogens

Nature Communications, 2023
CreTA, CRISPR-regulated toxin-antitoxin (TA), safeguards CRISPR-Cas immune systems by inducing cell dormancy/death upon their inactivation. Here, we characterize a bacterial CreTA associating with the I-F CRISPR-Cas in Acinetobacter.
Rui Wang, Xian Shu, Huiwei Zhao, Qiong Xue, Chao Liu, Aici Wu, Feiyue Cheng, Lingyun Wang, Yihan Zhang, Jie Feng, Nannan Wu, Ming Li   +11 more
doaj   +1 more source

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

Synthetic and Systems Biotechnology, 2018
CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and CRISPR-Cas12a, which have been most widely studied and ...
Ruilian Yao, Di Liu, Xiao Jia, Yuan Zheng, Wei Liu, Yi Xiao   +5 more
doaj   +1 more source

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes [PDF]

, 2017
Prokaryotes encode various host defense systems that provide protection against mobile genetic elements. Restriction-modification (R-M) and CRISPR-Cas systems mediate host defense by sequence specific targeting of invasive DNA.
Anders, C., Brouns, S.J.J., Dickman, M.J., Houkes, J., Jinek, M., Kunne, T., Lochs, S.J.A., Mohanraju, P., Swarts, D.C., van der Oost, J., Vlot, M., Zheng, P.   +11 more
core   +6 more sources

Development and Application of CRISPR/Cas System in Rice

Rice Science, 2019
In the past several years, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system has been harnessed as an efficient and powerful tool for targeted genome editing in different prokaryotic and ...
Ren Jun, Hu Xixun, Wang Kejian, Wang Chun   +3 more
doaj   +1 more source

Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein System for Resistance Against Plant Viruses: Applications and Perspectives

Frontiers in Plant Science, 2022
Different genome editing approaches have been used to engineer resistance against plant viruses. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas; CRISPR/Cas) systems to create pinpoint genetic ...
Fredy D. A. Silva, Elizabeth P. B. Fontes   +1 more
doaj   +1 more source

Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing

Nature Communications, 2023
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past decade and holds great promise for the treatment of human genetic diseases.
Jianli Tao, D. E. Bauer, R. Chiarle
semanticscholar   +1 more source

Characterization of 67 Confirmed Clustered Regularly Interspaced Short Palindromic Repeats Loci in 52 Strains of Staphylococci

Frontiers in Microbiology, 2021
Staphylococcus aureus (S. aureus), which is one of the most important species of Staphylococci, poses a great threat to public health. Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) are an ...
Ying Wang, Tingting Mao, Yinxia Li, Wenwei Xiao, Xuan Liang, Guangcai Duan, Haiyan Yang   +6 more
doaj   +1 more source

Clades of huge phages from across Earth's ecosystems. [PDF]

, 2020
Bacteriophages typically have small genomes1 and depend on their bacterial hosts for replication2. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of ...
Al-Shayeb, Basem, Amano, Yuki, Amundson, Ronald, Anantharaman, Karthik, Banfield, Jillian F, Borton, Mikayla A, Bouma-Gregson, Keith, Brooks, Brandon, Castelle, Cindy J, Cate, Jamie HD, Chen, Lin-Xing, Devoto, Audra, Doudna, Jennifer A, Farag, Ibrahim F, Finstad, Kari, Goltsman, Daniela SA, Harrison, Sue, He, Christine, Jaffe, Alexander L, Kantor, Rose, Keren, Ray, Lane, Katherine R, Lavy, Adi, Lehours, Anne-Catherine, Lei, Shufei, Li, Wen-Jun, Matheus-Carnevali, Paula, Morowitz, Michael, Munk, Patrick, Méheust, Raphaël, Nelson, Tara C, Olm, Matthew R, Power, Mary E, Probst, Alexander J, Relman, David A, Sachdeva, Rohan, Santini, Joanne M, Sharrar, Allison, Sun, Christine, Thomas, Alex, Tringe, Susannah G, Ward, Fred, Warren, Lesley, Wrighton, Kelly, Zhou, Jinglie   +44 more
core   +2 more sources