Results 41 to 50 of about 1,254,871 (374)
CRISPR-Cas-Based Antimicrobials: Design, Challenges, and Bacterial Mechanisms of Resistance
ACS Infectious Diseases, 2023 The emergence of antibiotic-resistant bacterial strains is a source of public health concern across the globe. As the discovery of new conventional antibiotics has stalled significantly over the past decade, there is an urgency to develop novel ...
Arianna Mayorga-Ramos +4 more
semanticscholar +1 more source
GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Nature Biotechnology, 2014 CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing reagents, but methods to delineate their genome-wide, off-target cleavage activities have been lacking.
S. Tsai +11 more
semanticscholar +1 more source
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice
Nature Biotechnology, 2023 Antibiotic treatments have detrimental effects on the microbiome and lead to antibiotic resistance. To develop a phage therapy against a diverse range of clinically relevant Escherichia coli , we screened a library of 162 wild-type (WT) phages ...
Y. E. Gençay +35 more
semanticscholar +1 more source
Special focus CRISPR-Cas [PDF]
RNA Biology, 2013 Six years ago a new sophisticated prokaryotic defense system was identified that was termed CRISPR-Cas (CRISPR, clustered regularly interspaced short palindromic repeats; Cas, CRISPR associated).1-...
openaire +2 more sources
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
Nature Materials, 2021 Endosomal escape remains a fundamental barrier hindering the advancement of nucleic acid therapeutics. Taking inspiration from natural phospholipids that comprise biological membranes, we report the combinatorial synthesis of multi-tailed ionizable ...
Shuai Liu +6 more
semanticscholar +1 more source
Adaptation in CRISPR-Cas Systems [PDF]
Molecular Cell, 2016 Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation.
Samuel H. Sternberg +3 more
openaire +4 more sources
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
Nucleic Acids Research, 2013 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic acids.
James E. DiCarlo +5 more
semanticscholar +1 more source
Dealing with the evolutionary downside of CRISPR immunity: bacteria and beneficial plasmids. [PDF]
PLoS Genetics, 2013 The immune systems that protect organisms from infectious agents invariably have a cost for the host. In bacteria and archaea CRISPR-Cas loci can serve as adaptive immune systems that protect these microbes from infectiously transmitted DNAs.
Wenyan Jiang +5 more
doaj +1 more source
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources