Results 51 to 60 of about 1,254,871 (374)
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
Nature Biotechnology, 2014 Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) are highly efficient genome editing tools. CRISPR-associated 9 (Cas9) RGNs are directed to genomic loci by guide RNAs (gRNAs) containing 20 nucleotides that ...
Yanfang Fu +4 more
semanticscholar +1 more source
CRISPR-Cas: Adapting to change
Science, 2017 Variation in prokaryote adaptive immunity To repel infection by phage and mobile genetic elements, prokaryotes have a form of adaptive immune response and memory invested in clustered regularly interspaced short palindromic repeats and associated proteins (CRISPR-Cas).
Jackson, Simon A. +5 more
openaire +5 more sources
Frontiers in Microbiology, 2018 Prokaryotic CRISPR-Cas systems limit the acquisition of genetic elements and provide immunity against invasive bacteriophage. The characteristics of CRISPR-Cas systems in clinical Klebsiella pneumoniae isolates are still unknown. Here, 97 K.
Hsin-Yu Li +8 more
doaj +1 more source
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture.
Annual Review of Plant Biology, 2019 Enhanced agricultural production through innovative breeding technology is urgently needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas genome editing enable efficient targeted modification in most crops, thus promising
Kunling Chen +4 more
semanticscholar +1 more source
CRISPR-Cas Systems in Streptococci [PDF]
Current Issues in Molecular Biology, 2019 Streptococci are one of the most important and common constituents of the host's microbiota and can colonize and live in the upper respiratory and urogenital tract of humans and animals. The CRISPR-Cas systems (i.e., clustered regularly interspaced short palindromic repeat, with CRISPR-associated proteins) found in bacteria and archaea provide sequence-
Tao, Gong +7 more
openaire +2 more sources
Research Progress on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)-Isothermal Amplification in the Detection of Foodborne Pathogens [PDF]
Shipin KexueFood safety incidents caused by foodborne pathogens have attracted widespread attention. In order to ensure food safety, it is essential to adopt effective detection means.
ZHANG Qi, PANG Lidong, SU Qunchao, SONG Danliangmin, YANG Xinyan, JIANG Yujun, ZHANG Wei
doaj +1 more source
Development and Application of CRISPR/Cas in Microbial Biotechnology
Frontiers in Bioengineering and Biotechnology, 2020 The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding +3 more
doaj +1 more source
Nature Communications, 2023 Approximately 10% of Cystic Fibrosis (CF) patients, particularly those with CF transmembrane conductance regulator (CFTR) gene nonsense mutations, lack effective treatments.
Tuo Wei +13 more
semanticscholar +1 more source
Optimization of CRISPR–Cas system for clinical cancer therapy
Bioengineering & Translational Medicine, 2023 Cancer is a genetic disease caused by alterations in genome and epigenome and is one of the leading causes for death worldwide. The exploration of disease development and therapeutic strategies at the genetic level have become the key to the treatment of
Xiang Meng +7 more
doaj +1 more source
Boosting plant immunity with CRISPR/Cas [PDF]
, 2015 CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method.See related Research: http://www.genomebiology.com/2015/16/1 ...
Chaparro-Garcia, Angela +2 more
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