Results 141 to 150 of about 58,663 (324)

Bilirubin Targeting WNK1 to Alleviate NLRP3‐Mediated Neuroinflammation

open access: yesAdvanced Science, EarlyView.
At physiological concentrations, bilirubin binds to the kinase domain of WNK1, thereby augmenting its activity and facilitating the phosphorylation of downstream SPAK/OSR1. This phosphorylation inhibits KCC2 activity, leading to elevate intracellular chloride levels in neurons.
Linfei Mao   +14 more
wiley   +1 more source

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system [PDF]

open access: hybrid, 2012
Kirill A. Datsenko   +5 more
openalex   +1 more source

Exploring AAV‐Mediated Gene Therapy for Inner Ear Diseases: from Preclinical Success to Clinical Potential

open access: yesAdvanced Science, EarlyView.
Current preclinical studies of AAV‐mediated gene therapy explore different strategies based on the characteristics of inner ear diseases. For genetic hearing loss, approaches include the replacement of a “good gene,” removal of a “bad gene,” or direct correction of mutations through base editing.
Fan Wu   +7 more
wiley   +1 more source

Optimal number of spacers in CRISPR arrays

open access: yes, 2017
We estimate the number of spacers in a CRISPR array of a bacterium which maximizes its protection against a viral attack. The optimality follows from a competition between two trends: too few distinct spacers make the bacteria vulnerable to an attack by ...
Ispolatov, Yaroslav   +2 more
core   +1 more source

Optimization of CRISPR-Cas systems in ectothermal organisms [PDF]

open access: yes, 2020
CRISPR-Cas systems have been traditionally optimized for gene editing in different types of models, such as in mammalian cell lines. However, their implementation in animal models requires additional work. In addition, another impediment is the number of
Moreno Mateos, Miguel Ángel   +1 more
core   +1 more source

NOX2 Contributes to High‐Frequency Outer Hair Cell Vulnerability in the Cochlea

open access: yesAdvanced Science, EarlyView.
This study first identifies NOX2 as a differentially expressed gene related to oxidative damage in the apical and basal turns through single‐cell RNA sequencing. NOX2 gene knockout mitigates OHCs damage caused by neomycin and noise and enhances Nrf2 expression and nuclear translocation.
Meihao Qi   +16 more
wiley   +1 more source

CRISPR RNA processing by a Type IV-A Cas6 endoribonuclease [PDF]

open access: yes, 2018
The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats - CRISPR ASsociated) system is an adaptive bacterial immune system. Upon infection by a foreign nucleic acid, such as phage, the bacterium\u27s CRISPR-Cas system takes a chunk of ...
Taylor, Hannah Nicole
core   +1 more source

AAVR Expression is Essential for AAV Vector Transduction in Sensory Hair Cells

open access: yesAdvanced Science, EarlyView.
Decreased sensitivity to AAV vector transduction in the outer hair cells (OHCs) of adult mice is primarily attributed to reduction of AAVR (Kiaa0319l; Au040320). Knockout of AAVR reduces AAV vector transduction efficiency in both inner hair cells (IHCs) and OHCs in neonatal mice.
Fan Wu   +8 more
wiley   +1 more source

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