Results 181 to 190 of about 58,663 (324)

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations

Advanced Science, EarlyView.
CRISPR/Cas9, while transformative, faces challenges in specificity, precision, delivery, accessibility, flexibility, and safety. This review addresses these limitations by highlighting strategies to reduce off‐target effects, exploring HDR‐based and alternative editing approaches, and evaluating advanced delivery mechanisms.
Muna Alariqi, Mohamed Ramadan, Lu Yu, Fengjiao Hui, Amjad Hussain, Xiaofeng Zhou, Yu Yu, Xianlong Zhang, Shuangxia Jin   +8 more
wiley   +1 more source

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems [PDF]

, 2015
Jiuyu Wang, Jiazhi Li, Hongtu Zhao, Gang Sheng, Min Wang, Maolu Yin, Yanli Wang   +6 more
openalex   +1 more source

From Code to Life: The AI‐Driven Revolution in Genome Editing

Advanced Science, EarlyView.
This review explores AI‐driven advancements in genome editing, focusing on CRISPR optimization, enhanced targeting precision, reduced off‐target effects, and novel tool design. It addresses ethical challenges, data biases, and highlights future prospects in precision medicine, agriculture, and synthetic biology.
Zhidong Li, Wasi Ullah Khan, Genxiang Bai, Chao Dong, Jungang Wang, Youpeng Zhang, Chong Wang, Hongbin Zhang, Wenyi Wang, Ming Luo, Fei Chen   +10 more
wiley   +1 more source

CRISPR-Cas Systems in the Cyanobacterium Synechocystis sp. PCC6803 Exhibit Distinct Processing Pathways Involving at Least Two Cas6 and a Cmr2 Protein

, 2013
Ingeborg Scholz, Sita J. Lange, Stephanie Hein, Wolfgang R. Hess, Rolf Backofen   +4 more
openalex   +2 more sources

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

Advanced Science, EarlyView.
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen, Junyuan Xue, Ziying Wang, Jinyuan Sun, Yinglu Cui, Tong Zhu, Huaiyi Yang, Ming Li, Bian Wu   +8 more
wiley   +1 more source

Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System

, 2013
Woong Y. Hwang, Yanfang Fu, Deepak Reyon, Morgan L. Maeder, Prakriti Kaini, Jeffry D. Sander, J. Keith Joung, Randall T. Peterson, Jing-Ruey Joanna Yeh   +8 more
openalex   +2 more sources

Survey of clustered regularly interspaced short palindromic repeats and their associated Cas proteins (CRISPR/Cas) systems in multiple sequenced strains of Klebsiella pneumoniae [PDF]

, 2015
Martha Lorena Ostria-Hernández, Carlos Javier Sánchez-Vallejo, J. Antonio Ibarra, Graciela Castro‐Escarpulli   +3 more
openalex   +1 more source

Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia‐Inducible Factor‐1 Signaling Pathway

Advanced Science, EarlyView.
This study investigates the role of macrophage pyruvate carboxylase (PC) in atherosclerosis (AS) demonstrating that PC upregulation in macrophages promotes metabolism reprogramming to enhance inflammatory responses via the HIF‐1 signaling pathway.
Ling‐Na Zhao, Rui‐Ling Wang, Ran‐Xin Liu, Meng‐Ru Zheng, Li Zhao, Bao‐Feng Li, Jia‐Le Li, De‐Shen Liu, Xiao‐Xia He, Qin‐Bao Peng, Kai Li, Tian‐Xiao Lin, Ying‐Ying Liu, Sheng‐Ping He, Jun Lu, Shao‐Yi Zheng, Xiu Liu, Fang‐Ze Huang   +17 more
wiley   +1 more source

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

, 2013
Reuben B. Vercoe, James T. Chang, Ron L. Dy, Corinda Taylor, Tamzin Gristwood, James S. Clulow, Corinna Richter, Rita Przybilski, Andrew R. Pitman, Peter C. Fineran   +9 more
openalex   +2 more sources

Dynamic DNA Target Proofreading in a CRISPR-Cas System [PDF]

, 2015
Marius Rutkauskas, Tomas Šinkūnas, Maria S. Tikhomirova, Virginijus Šikšnys, Ralf Seidel   +4 more
openalex   +1 more source