Results 211 to 220 of about 713,183 (311)

Model‐Guided Rational Construction of Escherichia coli Synthetic Consortia for Enhanced 2‐Methylbutyric Acid Production

Advanced Science, EarlyView.
We developed a citramalate‐derived pathway for 2‐methylbutyric acid production in E. coli mono‐culture and consortia. The optimized consortium, using 2‐keto‐3‐methylvalerate as a node, achieved 1817.03 ± 103.73 mg L−1 2‐methylbutyric acid, a 28‐fold increase from the initial strain, with a yield of 0.091 g/g glucose.
Yu Liu, Boyuan Xue, Shaojie Wang, Haijia Su   +3 more
wiley   +1 more source

Infinite-Dimensional System Signature: System Signature Under the Repairable Principle [PDF]

arXiv
Investigation of the reliability of technical systems is one of the application areas of stochastic processes. The reliability of a technical system is based on two main elements. The first is the connection type of the system, and the second is the distribution of the working times of the components consisting of the system.
arxiv  

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems [PDF]

, 2015
Jiuyu Wang, Jiazhi Li, Hongtu Zhao, Gang Sheng, Min Wang, Maolu Yin, Yanli Wang   +6 more
openalex   +1 more source

Cardiomyocyte‐Enriched USP20 Ameliorates Pathological Cardiac Hypertrophy by Targeting STAT3 Deubiquitination

Advanced Science, EarlyView.
Cardiomyocyte‐enriched USP20 regulates the K63‐linked ubiquitination of STAT3, and the cardiomyocyte‐specific USP20‐STAT3‐CARM1 axis exerts a protective role in cardiac hypertrophy. Targeting USP20 through cardiac‐specific gene therapy presents a promising strategy for the treatment of cardiac hypertrophy.
Lingfeng Zhong, Shanshan Dai, Fan Yu, Guo‐Ping Shi, Qinyan Gong, Yucong Zhang, Jingsi Duan, Zhengyin Lou, Zhixuan Tang, Fuzhe Gong, Derong Chen, Liya Hou, Xinyang Hu, Jinghai Chen, Jian'an Wang, Deling Yin   +15 more
wiley   +1 more source

Survey of clustered regularly interspaced short palindromic repeats and their associated Cas proteins (CRISPR/Cas) systems in multiple sequenced strains of Klebsiella pneumoniae [PDF]

, 2015
Martha Lorena Ostria-Hernández, Carlos Javier Sánchez-Vallejo, J. Antonio Ibarra, Graciela Castro‐Escarpulli   +3 more
openalex   +1 more source

Annexin A3 Represses Endothelial Permeability and Inflammation During Sepsis via Actin Cytoskeleton Modulation

Advanced Science, EarlyView.
Annexin A3 (ANXA3) plays an endogenous protective role in endothelial dysfunction in sepsis by stabilizing the actin cytoskeleton, modulating cell permeability, and inhibiting pATF2/CD62E inflammatory signaling. Abstract Increased endothelial permeability and a dysregulated inflammatory response play key roles in organ damage in sepsis.
Manyu Xing, Shuang Liang, Wei Cao, Qulian Guo, Wangyuan Zou   +4 more
wiley   +1 more source

Dynamic DNA Target Proofreading in a CRISPR-Cas System [PDF]

, 2015
Marius Rutkauskas, Tomas Šinkūnas, Maria S. Tikhomirova, Virginijus Šikšnys, Ralf Seidel   +4 more
openalex   +1 more source

ALKBH3‐Mediated M1A Demethylation of METTL3 Endows Pathological Fibrosis:Interplay Between M1A and M6A RNA Methylation

Advanced Science, EarlyView.
RNA modification is crucial in fibrosis diseases, but the role of m1A modification remains elusive. This study reveals that ALKBH3, an m1A demethylase, drives skin fibrosis by reshaping m6A RNA modification and stabilizing COL1A1 and FN1 mRNAs through METTL3, uncovering a crosstalk between m1A and m6A methylation in pathological events.
Liying Tu, Shuchen Gu, Ruoqing Xu, En Yang, Xin Huang, Hsin Liang, Shenying Luo, Haizhou Li, Yixuan Zhao, Tao Zan   +9 more
wiley   +1 more source

CRISPR-Cas Systems in the Cyanobacterium Synechocystis sp. PCC6803 Exhibit Distinct Processing Pathways Involving at Least Two Cas6 and a Cmr2 Protein

, 2013
Ingeborg Scholz, Sita J. Lange, Stephanie Hein, Wolfgang R. Hess, Rolf Backofen   +4 more
openalex   +2 more sources

Enhanced the Trans‐Cleavage Activity of CRISPR‐Cas12a Using Metal‐Organic Frameworks as Stimulants for Efficient Electrochemical Sensing of Circulating Tumor DNA

Advanced Science, EarlyView.
This work designs manganese metal‐organic frameworks (Mn‐MOFs) co‐assembled with Cas12a/crRNA, enabling rapid release of Mn2⁺ under mild conditions. The MOFs‐induced proximity effect sustains Mn2⁺‐Cas12a/crRNA interactions, enhancing the trans‐cleavage activity of complex available to develop an electrochemical biosensor based on Mn‐MOFs‐enhanced ...
Shuai Wu, Yincheng Liu, Tianyu Zeng, Tianci Zhou, Yanting Sun, Ying Deng, Juan Zhang, Genxi Li, Yongmei Yin   +8 more
wiley   +1 more source