Results 221 to 230 of about 39,519 (255)

Current understanding of the cyanobacterial CRISPR-Cas systems and development of the synthetic CRISPR-Cas systems for cyanobacteria

Enzyme and Microbial Technology, 2020
Cyanobacteria are photosynthetic microorganisms that are capable of converting CO2 to value-added chemicals. Engineering of cyanobacteria with synthetic biology tools, including the CRISPR-Cas system, has allowed an opportunity for biological CO2 utilization.
Napisa Pattharaprachayakul, Mieun Lee, Aran Incharoensakdi, Han Min Woo   +3 more
openaire   +2 more sources

CRISPR-Cas systems in Serratia

2022
The CRISPR-Cas system of Prokaryotes is an adaptive immune defense mechanism to protect themselves from invading genetic elements (e.g. phages and plasmids). Studies that describe the genetic organization of these prokaryotic systems have mainly reported on the Enterobacteriaceae family (now reorganized within the order Enterobacteriales).
Maria Scrascia, Roberta Roberto, Pietro Daddabbo, Yosra Ahmed, Francesco Porcelli, Marta Oliva, Carla Calia, Angelo Marzella, Carlo Pazzani   +8 more
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An introduction and use of the CRISPR-Cas systems

2021
Clusters of regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins (Cas) system (CRISPR-Cas) is a rapidly evolving field of targeted genome engineering. The type II CRISPR-Cas9 is used for genome editing of many organisms. Single guide RNA (sgRNA) can bind to Cas9 protein that can target desired sequences in presence of
openaire   +2 more sources

CRISPR/Cas System: An Introduction

2021
The process of altering genetic code to modify DNA precisely and efficiently within a cell is known as genome editing. All techniques used for changing a gene in a cell are based on cutting of double-stranded DNA through DNA endonucleases at specific site.
Nayla Munawar, Aftab Ahmad
openaire   +1 more source

CRISPR-Cas Systems

2013
Discovery and seminal developments in the CRISPR field.- Occurrence, diversity of CRISPR-Cas systems and genotyping implications.- Evolution and classification of CRISPR-Cas systems and Cas protein families.- Regulation of CRISPR-based immune responses.- crRNA Biogenesis.- Distribution and mechanism of the Type I CRISPR/Cas Systems.- Type II ...
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The CRISPR-Cas system in molecular diagnostics

Clinica Chimica Acta
Robust, sensitive, and rapid molecular detection tools are essential prerequisites for disease diagnosis and epidemiological control. However, the current mainstream tests necessitate expensive equipment and specialized operators, impeding the advancement of molecular diagnostics. The CRISPR-Cas system is an integral component of the bacterial adaptive
Rao, Song, Zhongyi, Chen, Hongtao, Xiao, Haojun, Wang   +3 more
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Advances in CRISPR-Cas systems for epigenetics

The CRISPR-Cas9 method has revolutionized the gene editing. Epigenetic changes, including DNA methylation, RNA modification, and changes in histone proteins, have been intensively studied and found to play a key role in the pathogenesis of human diseases.
Mahnoor, Ilyas, Qasim, Shah, Alvina, Gul, Huzaifa, Ibrahim, Rania, Fatima, Mustafeez Mujtaba, Babar, Jayakumar, Rajadas   +6 more
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The ring of confidence: a haloarchaeal CRISPR/Cas system

Biochemical Society Transactions, 2013
To survive the constant invasions by foreign genetic elements, prokaryotes have evolved various defensive systems. Almost all sequenced archaea, and half of the analysed bacteria use the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system, a recently identified prokaryotic immune system that can fend off ...
Maier, L., Stoll, B., Brendel, J., Fischer, S., Pfeiffer, F., Dyall-Smith, M., Marchfelder, A.   +6 more
openaire   +3 more sources

Alternative functions of CRISPR–Cas systems in the evolutionary arms race

Nature Reviews Microbiology, 2022
John Van Der Oost, Prarthana Mohanraju, Raymond Staals   +2 more
exaly  

Structural biology of CRISPR–Cas immunity and genome editing enzymes

Nature Reviews Microbiology, 2022
Patrick Pausch, Jennifer Doudna, Joy Wang   +2 more
exaly