Results 281 to 290 of about 58,663 (324)

Dual Detection of Hepatitis B and C Viruses Using CRISPR-Cas Systems and Lateral Flow Assay. [PDF]

J Nucleic Acids
Shahni SN, Albogami S, Azmi I, Pattnaik B, Chaudhuri R, Dev K, Iqbal J, Sharma A, Ahmad T.   +8 more
europepmc   +1 more source

Engineering Adenine Deaminase TadA for Precise and PAM‐Flexible Point Mutagenesis and Gradient‐Tuning Endogenous Protein Design

Advanced Science, EarlyView.
Base editing enables precise nucleotide substitutions but limited by bystander editing. This study engineers plant base editors by fusing optimized TadA variants with PAM‐flexible SpRY nickase, enabling A‐to‐G, C‐to‐T, and dual‐base conversions in a highly condensed window (≤3 nucleotides). Additionally, TadDBE (TadA Dual‐Base Editor)‐mediated directed
Kangli Sun, Si Cheng, Nan Chai, Jianing Mi, Ruixiang Zhang, Qian Qian, Zhiye Zheng, Ke Chen, Dongchang Zeng, Xin Peng, Mengyuan Shen, Degui Zhou, Qinlong Zhu, Qi Liu, Jiantao Tan   +14 more
wiley   +1 more source

Intricate interplay of CRISPR-Cas systems, anti-CRISPR proteins, and antimicrobial resistance genes in a globally successful multi-drug resistant Klebsiella pneumoniae clone. [PDF]

Genome Med
Jiang J, Cienfuegos-Gallet AV, Long T, Peirano G, Chu T, Pitout JDD, Kreiswirth BN, Chen L.   +7 more
europepmc   +1 more source

FOXM1 Protects Against Myocardial Ischemia‐Reperfusion Injury in Rodent and Porcine Models by Suppressing MKRN1‐Dependent LKB1 Ubiquitination

Advanced Science, EarlyView.
FOXM1 maintains mitochondrial bioenergetic function by inhibiting MKRN1‐mediated ubiquitination of LKB1 in cardiomyocytes. Loss of FOXM1 in cardiomyocytes results in upregulation of MKRN1, which enhances LKB1 ubiquitination and disrupts AMPK signaling and energy metabolism pathways. Conversely, FOXM1 overexpression preserves mitochondrial bioenergetics
Shuai Song, Xiaokai Zhang, Zihang Huang, Zhiqiang Pei, Linqi Zeng, Fengze Cai, Tongyao Wang, Mohan Li, Chenyan Liu, Yining Song, Jiahao Guo, Hao Lu, Xinyu Weng, Li Shen, Xiaochun Zhang, Xingxing Cai, Aijun Sun, Junbo Ge   +17 more
wiley   +1 more source

Correction: Intricate interplay of CRISPR-Cas systems, anti-CRISPR proteins, and antimicrobial resistance genes in a globally successful multi-drug resistant Klebsiella pneumoniae clone. [PDF]

Genome Med
Jiang J, Cienfuegos-Gallet AV, Long T, Peirano G, Chu T, Pitout JDD, Kreiswirth BN, Chen L.   +7 more
europepmc   +1 more source

Rapid and Ultra‐Sensitive SARS‐CoV‐2 Subgenomic RNA Detection Using Single‐Molecule With a Large Transistor‐SiMoT Bioelectronic Platform

Advanced Electronic Materials, EarlyView.
The single‐molecule with a large transistor‐SiMoT is proposed for the detection of the subgenomic RNA of SARS‐CoV‐2, achieving a diagnostic sensitivity of 98.0% and a specificity of 87.8%. The SiMoT technology, currently at TRL‐5, is suitable for point‐of‐care settings and delivers the result to the end user in 30 min.
Eleonora Macchia, Anna Maria D'Erchia, Mariapia Caputo, Angelica Bianco, Claudia Leoni, Francesca Intranuovo, Cecilia Scandurra, Lucia Sarcina, Cinzia Di Franco, Paolo Bollella, Gaetano Scamarcio, Luisa Torsi, Graziano Pesole   +12 more
wiley   +1 more source

Electronic Nanomaterials for Plants: A Review on Current Advances and Future Prospects

Advanced Electronic Materials, EarlyView.
Global food security faces mounting challenges from climate change and rapid population growth. This review highlights the pivotal role of electronic nanomaterials–including metals, metal oxides, and carbon‐based structures–in enhancing plant photosynthesis, nutrient uptake, and stress resilience. Furthermore, it explores how emerging platforms such as
Ciro Allará, Giuseppe Ciccone, Manuela Ciocca, Sahira Vasquez, Pietro Ibba, Mauro Maver, Tanja Mimmo, Paolo Lugli, Luisa Petti   +8 more
wiley   +1 more source

Correction to 'DNA target binding-induced pre-crRNA processing in type II and V CRISPR-Cas systems'. [PDF]

Nucleic Acids Res
europepmc   +1 more source

Harnessing the Biological Responses Induced by Nanomaterials for Enhanced Cancer Therapy

Aggregate, EarlyView.
Nanomaterial (NM)‐induced toxicity can be strategically repurposed for cancer therapy. This review summarizes the mechanism by which NMs selectively activate specific cellular processes to regulate cell fate independently. We also discussed how NMs‐induced biological responses can be leveraged as therapeutic strategies for cancer treatment.
Liting Wang, Hao Fu, Jiangtao Lin, Meng Zhao, Chuanrong Chen, Hongze Liao, Yourong Duan   +6 more
wiley   +1 more source

Comparative analysis of two newly established Cre rat lines, NeuN‐Cre and Thy1‐Cre, for neurological research

Animal Models and Experimental Medicine, EarlyView.
We generated two neuron‐specific Cre rat lines, Thy1‐Cre and NeuN‐Cre, using CRISPR/Cas9‐mediated targeted insertion. Both lines were validated for high neural specificity by crossing them with a Cre reporter rat. When bred with an ND1 conditional knockout rat, the two Cre lines produced distinct phenotypic outcomes. These rat lines have been deposited
Keru Li, Chenyang Liu, Gefan Wan, Lei Zhang, Rui Fan, Xu Zhang, Yuanlong Su, Jiayue He, Ning Liu, Feifei Guan, Wei Dong, Shan Gao, Weining Kong, Xiaolong Qi, Xiaolin Zhang, Yuanwu Ma   +15 more
wiley   +1 more source