Results 71 to 80 of about 58,663 (324)

CRISPR-Cas systems in enterococci

Brazilian Journal of Microbiology
Enterococci are members of the microbiota of humans and other animals. They can also be found in the environment, associated with food, healthcare infections, and hospital settings. Due to their wide distribution, they are inserted in the One Health context.
Amanda Seabra Cabral, Fernanda de Freitas Lacerda, Vitor Luis Macena Leite, Filipe Martire de Miranda, Amanda Beiral da Silva, Bárbara Araújo dos Santos, Jailton Lobo da Costa Lima, Lúcia Martins Teixeira, Felipe Piedade Gonçalves Neves   +8 more
openaire   +2 more sources

Substrate Generation for Endonucleases of CRISPR/Cas Systems [PDF]

Journal of Visualized Experiments, 2012
The interaction of viruses and their prokaryotic hosts shaped the evolution of bacterial and archaeal life. Prokaryotes developed several strategies to evade viral attacks that include restriction modification, abortive infection and CRISPR/Cas systems. These adaptive immune systems found in many Bacteria and most Archaea consist of clustered regularly
Judith Zoephel, Hagen Richter, Srivatsa Dwarakanath, André Plagens, Lennart Randau   +4 more
openaire   +3 more sources

Efficient In Vivo Genome Editing Using RNA-Guided Nucleases [PDF]

, 2014
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have evolved in bacteria and archaea as a defense mechanism to silence foreign nucleic acids of viruses and plasmids.
Fu, Yanfang, Hwang, Woong Y., Joung, J. Keith, Maeder, Morgan L., Peterson, Randall T., Reyon, Deepak, Sander, Jeffry D., Tsai, Shengdar Q., Yeh, J.-R. Joanna   +8 more
core   +1 more source

CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]

, 2020
The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I, Jacinto, Filipe, Link, Wolfgang   +2 more
core   +1 more source

Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant Pseudomonas aeruginosa

mBio, 2015
Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for understanding the molecular mechanisms underlying type I clustered regularly ...
Alex van Belkum, Leah B. Soriaga, Matthew C. LaFave, Srividya Akella, Jean-Baptiste Veyrieras, E. Magda Barbu, Dee Shortridge, Bernadette Blanc, Gregory Hannum, Gilles Zambardi, Kristofer Miller, Mark C. Enright, Nathalie Mugnier, Daniel Brami, Stéphane Schicklin, Martina Felderman, Ariel S. Schwartz, Toby H. Richardson, Todd C. Peterson, Bolyn Hubby, Kyle C. Cady   +20 more
doaj   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

CRISPR-associated sequence diversity within Xanthomonas albilineans, the causal agent of leaf scald disease of sugarcane. [PDF]

, 2012
Xanthomonas albilineans is a xylem-invading pathogen that causes leaf scald, a lethal disease of sugarcane. Unlike other xanthomonads, X. albilineans exhibits a large intra-species variability which was previously observed with different genetic markers (
Bolot, Stéphanie, Carrère, Sébastien, Cociancich, Stéphane, Gouzy, Jérôme, Marguerettaz, Mélanie, Pieretti, Isabelle, Rott, Philippe, Royer, Monique   +7 more
core  

CRISPR Content Correlates with the Pathogenic Potential of Escherichia coli [PDF]

, 2015
Guide RNA molecules (crRNA) produced from clustered regularly interspaced short palindromic repeat (CRISPR) arrays, altogether with effector proteins (Cas) encoded by cognate cas (CRISPR associated) genes, mount an interference mechanism (CRISPR-Cas ...
Almendros, Cristóbal, García-Gutiérrez, Enriqueta, García-Martínez, Jesús, Guzmán, Noemí M., Mojica, Francisco J.M.   +4 more
core   +4 more sources

Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]

, 2020
Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A, Fellmann, Christof, Lew, Rachel J, McMahon, Blake, Shivram, Haridha, Watters, Kyle E   +5 more
core   +3 more sources

COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758

Acta Biomedica Scientifica, 2018
Background. Pseudotuberculosis is still relevant problem in medical science and public health of Russia and other countries. Typing of Y. рseudotuberculosis strains by their CRISPR systems is a perspective tool for monitoring of Yersinia populations as ...
N. P. Peretolchina, A. Y. Borisenko, Yu. P. Dzhioev, V. I. Zlobin   +3 more
doaj   +1 more source