Results 51 to 60 of about 64,886 (293)
Biology Direct, 2011 Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to ...
Wolf Yuri I +3 more
doaj +1 more source
CRISPR-associated sequence diversity within Xanthomonas albilineans, the causal agent of leaf scald disease of sugarcane. [PDF]
, 2012 Xanthomonas albilineans is a xylem-invading pathogen that causes leaf scald, a lethal disease of sugarcane. Unlike other xanthomonads, X. albilineans exhibits a large intra-species variability which was previously observed with different genetic markers (
Bolot, Stéphanie +7 more
core +2 more sources
Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus
Frontiers in Microbiology, 2023 Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S.
Richard Bauer +5 more
doaj +1 more source
SnapShot: Class 1 CRISPR-Cas Systems
Cell, 2017 Class 1 CRISPR-Cas systems are characterized by effector modules consisting of multiple subunits. Class 1 systems comprise about 90% of all CRISPR-Cas loci identified in bacteria and archaea and can target both DNA and RNA.
Kira S, Makarova +2 more
openaire +2 more sources
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
The structural biology of CRISPR-Cas systems [PDF]
Current Opinion in Structural Biology, 2015 Prokaryotic CRISPR-Cas genomic loci encode RNA-mediated adaptive immune systems that bear some functional similarities with eukaryotic RNA interference. Acquired and heritable immunity against bacteriophage and plasmids begins with integration of ∼30 base pair foreign DNA sequences into the host genome.
Jiang, Fuguo, Doudna, Jennifer A
openaire +5 more sources
CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges
HeliyonCRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 and CRISPR/Cas3 utilize different mechanisms to detect or ...
Jian Zhou +3 more
doaj +1 more source
CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli [PDF]
, 2013 Prokaryotes immunize themselves against transmissible genetic elements by the integration (acquisition) in clustered regularly interspaced short palindromic repeats (CRISPR) loci of spacers homologous to invader nucleic acids, defined as protospacers ...
Almendros, Cristóbal +4 more
core +2 more sources
Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant
mBio, 2015 Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for understanding the molecular mechanisms underlying type I clustered regularly ...
Alex van Belkum +20 more
doaj +1 more source
In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus [PDF]
, 2013 Clustered regularly interspaced short palindromic repeats (CRISPR)-encoded immunity in Type I systems relies on the Cascade (CRISPR-associated complex for antiviral defence) ribonucleoprotein complex, which triggers foreign DNA degradation by an ...
Giedrius Gasiunas +8 more
core +2 more sources