Results 41 to 50 of about 10,050,452 (244)
Acta Biomedica Scientifica, 2018 Background. Pseudotuberculosis is still relevant problem in medical science and public health of Russia and other countries. Typing of Y. рseudotuberculosis strains by their CRISPR systems is a perspective tool for monitoring of Yersinia populations as ...
N. P. Peretolchina +3 more
doaj +1 more source
Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9 [PDF]
, 2014 This work was supported in part by NIH grant 1DP5OD017892 to S.LToxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the
Saima M Sidik +14 more
core +2 more sources
, 2022 In this project we have searched for genes associated with CRISPR-Cas systems from bacteria of the ESKAPE group using Random Forest and we have been able to reduce the percentage of CRISPR black matter, as well as to propose a triad 'Membrane Proteins ...
Rubio, Alejandro +3 more
core +1 more source
Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant
mBio, 2015 Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for understanding the molecular mechanisms underlying type I clustered regularly ...
Alex van Belkum +20 more
doaj +1 more source
CRISPR-Cas Controls Cryptic Prophages
, 2022 The bacterial archetypal adaptive immune system, CRISPR-Cas, is thought to be repressed in the best-studied bacterium, Escherichia coli K-12. We show here that the E.
Severinov, Konstantin +20 more
core +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
CRISPR-Cas-Based Engineering of Probiotics
BioDesign Research, 2023 Probiotics are the treasure of the microbiology fields. They have been widely used in the food industry, clinical treatment, and other fields. The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further ...
Ling Liu, Shimaa Elsayed Helal, Nan Peng
doaj +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Mechanisms regulating the CRISPR-Cas systems
Frontiers in Microbiology, 2023 The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats- CRISPR associated proteins) is a prokaryotic system that enables sequence specific recognition and cleavage of nucleic acids.
Marta Zakrzewska +3 more
doaj +1 more source
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source