Results 41 to 50 of about 64,886 (293)

New CRISPR–Cas systems from uncultivated microbes [PDF]

Nature, 2016
CRISPR-Cas systems provide microbes with adaptive immunity by employing short DNA sequences, termed spacers, that guide Cas proteins to cleave foreign DNA. Class 2 CRISPR-Cas systems are streamlined versions, in which a single RNA-bound Cas protein recognizes and cleaves target sequences.
Burstein, David, Harrington, Lucas B, Strutt, Steven C, Probst, Alexander J, Anantharaman, Karthik, Thomas, Brian C, Doudna, Jennifer A, Banfield, Jillian F   +7 more
openaire   +5 more sources

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes [PDF]

, 2017
Prokaryotes encode various host defense systems that provide protection against mobile genetic elements. Restriction-modification (R-M) and CRISPR-Cas systems mediate host defense by sequence specific targeting of invasive DNA.
Anders, C., Brouns, S.J.J., Dickman, M.J., Houkes, J., Jinek, M., Kunne, T., Lochs, S.J.A., Mohanraju, P., Swarts, D.C., van der Oost, J., Vlot, M., Zheng, P.   +11 more
core   +3 more sources

Characterization of CRISPR-Cas Systems in Clinical Klebsiella pneumoniae Isolates Uncovers Its Potential Association With Antibiotic Susceptibility

Frontiers in Microbiology, 2018
Prokaryotic CRISPR-Cas systems limit the acquisition of genetic elements and provide immunity against invasive bacteriophage. The characteristics of CRISPR-Cas systems in clinical Klebsiella pneumoniae isolates are still unknown. Here, 97 K.
Hsin-Yu Li, Cheng-Yen Kao, Wei-Hung Lin, Po-Xing Zheng, Jing-Jou Yan, Ming-Cheng Wang, Ching-Hao Teng, Chin-Chung Tseng, Jiunn-Jong Wu   +8 more
doaj   +1 more source

CRISPR-Cas-Docker: web-based in silico docking and machine learning-based classification of crRNAs with Cas proteins

BMC Bioinformatics, 2023
Background CRISPR-Cas-Docker is a web server for in silico docking experiments with CRISPR RNAs (crRNAs) and Cas proteins. This web server aims at providing experimentalists with the optimal crRNA-Cas pair predicted computationally when prokaryotic ...
Ho-min Park, Jongbum Won, Yunseol Park, Esla Timothy Anzaku, Joris Vankerschaver, Arnout Van Messem, Wesley De Neve, Hyunjin Shim   +7 more
doaj   +1 more source

Drug Inducible CRISPR/Cas Systems

Computational and Structural Biotechnology Journal, 2019
Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have been employed as a powerful versatile technology for programmable gene editing, transcriptional modulation, epigenetic modulation, and genome labeling, etc.
Jingfang Zhang, Li Chen, Ju Zhang, Yu Wang   +3 more
openaire   +3 more sources

Applications of CRISPR–Cas systems in neuroscience [PDF]

, 2016
Genome-editing tools, and in particular those based on CRISPR-Cas (clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein) systems, are accelerating the pace of biological research and enabling targeted genetic ...
A Bolotin, A Fire, A Geling, A Plessis, A Xiao, A Zeisel, AJ Wood, AM Geurts, AN Shah, AW Chan, AY Cheung, B Chen, B Pardo, B Zetsche, B Zetsche, C Burger, C Pak, C Straub, D Kim, D Maddalo, D St Johnston, DB Cox, DC van Gent, E Deltcheva, E Sasaki, EA Brenowitz, EB Lewis, EE Patton, EM Jorgensen, F Liang, F Soldner, F Zhang, FA Ran, FA Ran, FD Urnov, Feng Zhang, G Gasiunas, G Murlidharan, GP Flowers, H Akil, H Liu, H Wang, H Xia, HB Kordasiewicz, I Harel, I Jeon, IB Hilton, ID Carbery, IE Clark, J Boch, J Fishman-Lobell, J Fishman-Lobell, J Morton, J Peters, JA Harris, JA Zuris, JC Belmonte, JC Miller, JD Hommel, JD Sander, JD Sander, JD Sweatt, JE Garneau, JL Bessereau, JM Taymans, KJ Beumer, KR Thomas, KS Makarova, KY Kim, L Cong, L Cooley, L Swiech, LA Gilbert, LE Dow, LE Jao, LR Polstein, LS Qi, M Bibikova, M Bibikova, M Christian, M Garriga-Canut, M Jinek, M Maresca, M Zuckermann, Matthias Heidenreich, MC Marchetto, MJ Moscou, N Gaiano, N Rudin, N Wittenburg, NA Kearns, P Essletzbichler, P Mali, P Mali, P Qiu, P Rouet, P Rouet, PD Hsu, R Barrangou, RA Smith, RB Blasco, RD Johnson, RJ Platt, RR Beerli, S Incontro, S Konermann, S Konermann, S Oddo, SD Ryan, SH Yang, SM Elbashir, SQ Tsai, T Katsuyama, T Yagi, TO Auer, VM Bedell, Y Doyon, Y Fu, Y Niu, YG Kim, Z Shen   +120 more
core   +1 more source

CRISPR-Cas in Acinetobacter baumannii Contributes to Antibiotic Susceptibility by Targeting Endogenous AbaI

Microbiology Spectrum, 2022
Acinetobacter baumannii is a well-known human opportunistic pathogen in nosocomial infections, and the emergence of multidrug-resistant Acinetobacter baumannii has become a complex problem for clinical anti-infective treatments.
Yuhang Wang, Jie Yang, Xiaoli Sun, Mengying Li, Pengyu Zhang, Zhongtian Zhu, Hongmei Jiao, Tingting Guo, Guocai Li   +8 more
doaj   +1 more source

Therapeutic Applications of the CRISPR-Cas System

Bioengineering, 2022
The clustered regularly interspaced palindromic repeat (CRISPR)-Cas system has revolutionized genetic engineering due to its simplicity, stability, and precision since its discovery. This technology is utilized in a variety of fields, from basic research in medicine and biology to medical diagnosis and treatment, and its potential is unbounded as new ...
Kyungmin Kang, Youngjae Song, Inho Kim, Tae-Jung Kim   +3 more
openaire   +3 more sources

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems [PDF]

, 2017
Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and Cpf1, are known.
Abudayyeh, Omar Osama, Gootenberg, Jonathan S, Joung, Julia, Konermann, Silvana M, Koonin, Eugene V., Makarova, Kira S., Minakhin, Leonid, Semenova, Ekaterina, Severinov, Konstantin, Shmakov, Sergey, Wolf, Yuri I., Zhang, Feng   +11 more
core   +1 more source

Adaptation by Type III CRISPR-Cas Systems: Breakthrough Findings and Open Questions

Frontiers in Microbiology, 2022
CRISPR-Cas systems acquire heritable defense memory against invading nucleic acids through adaptation. Type III CRISPR-Cas systems have unique and intriguing features of defense and are important in method development for Genetics research. We started to
Xinfu Zhang, Xinfu Zhang, Xinmin An
doaj   +1 more source