Results 31 to 40 of about 39,519 (255)
The structural biology of CRISPR-Cas systems [PDF]
Current Opinion in Structural Biology, 2015 Prokaryotic CRISPR-Cas genomic loci encode RNA-mediated adaptive immune systems that bear some functional similarities with eukaryotic RNA interference. Acquired and heritable immunity against bacteriophage and plasmids begins with integration of ∼30 base pair foreign DNA sequences into the host genome.
Jiang, Fuguo, Doudna, Jennifer A
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Nanocarriers: A novel strategy for the delivery of CRISPR/Cas systems
Frontiers in Chemistry, 2022 In recent decades, clustered regularly interspaced short palindromic repeat/CRISPR-associated protein (CRISPR/Cas) has become one of the most promising genome-editing tools for therapeutic purposes in biomedical and medical applications.
Faranak Hejabi +5 more
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CRISPR-Cas Systems Reduced to a Minimum [PDF]
Molecular Cell, 2019 In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.
Almendros, Cristóbal +2 more
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Epidemiological and evolutionary consequences of different types of CRISPR-Cas systems.
PLoS Computational Biology, 2022 Bacteria have adaptive immunity against viruses (phages) in the form of CRISPR-Cas immune systems. Currently, 6 types of CRISPR-Cas systems are known and the molecular study of three of these has revealed important molecular differences. It is unknown if
Hélène Chabas +3 more
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Frontiers in Microbiology, 2018 Prokaryotic CRISPR-Cas systems limit the acquisition of genetic elements and provide immunity against invasive bacteriophage. The characteristics of CRISPR-Cas systems in clinical Klebsiella pneumoniae isolates are still unknown. Here, 97 K.
Hsin-Yu Li +8 more
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BMC Bioinformatics, 2023 Background CRISPR-Cas-Docker is a web server for in silico docking experiments with CRISPR RNAs (crRNAs) and Cas proteins. This web server aims at providing experimentalists with the optimal crRNA-Cas pair predicted computationally when prokaryotic ...
Ho-min Park +7 more
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Strategies for High-Efficiency Mutation Using the CRISPR/Cas System
Frontiers in Cell and Developmental Biology, 2022 Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems have revolutionized traditional gene-editing tools and are a significant tool for ameliorating gene defects.
Shuying Feng +10 more
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Microbiology Spectrum, 2022 Acinetobacter baumannii is a well-known human opportunistic pathogen in nosocomial infections, and the emergence of multidrug-resistant Acinetobacter baumannii has become a complex problem for clinical anti-infective treatments.
Yuhang Wang +8 more
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Origins and evolution of CRISPR-Cas systems [PDF]
Philosophical Transactions of the Royal Society B: Biological Sciences, 2019 CRISPR-Cas, the bacterial and archaeal adaptive immunity systems, encompass a complex machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic elements (MGE), into CRISPR arrays embedded in microbial genomes. Transcripts of the inserted segments (spacers) are employed by CRISPR-Cas systems as guide (g)RNAs for ...
Eugene V. Koonin, Kira S. Makarova
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Adaptation by Type III CRISPR-Cas Systems: Breakthrough Findings and Open Questions
Frontiers in Microbiology, 2022 CRISPR-Cas systems acquire heritable defense memory against invading nucleic acids through adaptation. Type III CRISPR-Cas systems have unique and intriguing features of defense and are important in method development for Genetics research. We started to
Xinfu Zhang, Xinfu Zhang, Xinmin An
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