Results 71 to 80 of about 10,050,452 (244)

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

BIOINFORMATIC SEARCH OF CRISPR/CAS SYSTEM STRUCTURES IN GENOME OF PCT281 PLASMID OF BACILLUS THURINGIENSIS SUBSP. CHINENSIS STRAIN CT-43

Acta Biomedica Scientifica, 2018
Background. CRISPR/Cas systems loci are one of the functionally important patterns in bacterial genome which perform the role of “adaptive immune defense” from foreign nucleic acids.
N. A. Arefyeva, Yu. P. Dzhioev, A. Yu. Borisenko, V. I. Chemerilova, O. F. Vyatchina, O. A. Sekerina, L. A. Stepanenko, Yu. A. Markova, G. V. Yurinova, V. P. Salovarova, A. A. Pristavka, V. A. Kuzminova, O. N. Reva, V. I. Zlobin   +13 more
doaj   +1 more source

Using CRISPR-Cas systems as antimicrobials

, 2017
International audienceAlthough CRISPR-Cas systems naturally evolved to provide adaptive immunity in bacteria and archaea, Cas nucleases can be co-opted to target chromosomal sequences rather than invasive genetic elements.
David Bikard, Bikard, David, Barrangou, Rodolphe, Rodolphe Barrangou   +3 more
core   +1 more source

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Development and Application of CRISPR/Cas System in Rice

Rice Science, 2019
In the past several years, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system has been harnessed as an efficient and powerful tool for targeted genome editing in different prokaryotic and ...
Ren Jun, Hu Xixun, Wang Kejian, Wang Chun   +3 more
doaj   +1 more source

Somatostatin receptor 4 (SSTR4) is a tumor suppressor in cutaneous and head & neck squamous cell carcinomas

Molecular Oncology, EarlyView.
This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi, Tuan Khang Huynh, Adway Kadam, Zahra Kardan, Yue Yan, Amina Jamal Laham, Sampath Kumar Loganathan   +6 more
wiley   +1 more source

NAPRT loss promotes lung tumor initiation and growth through AKT signaling independently of NAD+ biosynthesis

Molecular Oncology, EarlyView.
Loss of NAPRT promotes lung tumor initiation and growth through a noncanonical mechanism, independent of its role in NAD+ biosynthesis. Mechanistically, NAPRT depletion activates the mTORC2‐driven AKT/β‐catenin signaling axis to enhance clonogenic and invasive phenotypes. Furthermore, lung‐specific Naprt deletion significantly increases tumor burden in
Myung Joon Oh, Ji Yun Jang, Yoon Jeon, Gi‐Cheon Kim, Hwanho Lee, Jooyoung Lee, Ju‐Hwa Kim, Hakhyun Kim, Minjee Kim, Ho‐Keun Kwon, Ho Lee, Hyun Seok Kim   +11 more
wiley   +1 more source

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
Beijer, Marieke R., Mark J Dickman, Dickman, Mark J.,, Snijders, AP, Matthijs M Jore, Rolf Wagner, Sakharam P Waghmare, Beijer, Marieke, Wagner, Rolf, Brouns, Stan J. J., Barendregt, Arjan,, Snijders, Ambrosius, Dickman, Mark, Oost, J., van der, Arjan Barendregt, Boekema, Egbert, Dickman, Mark J., Bultema, J.B., Boekema, EJ, Waghmare, Sakharam P.,, Brouns, S.J.J., Wurm, Reinhild,, Westra, ER, Pul, U, Boekema, Egbert J., Doudna, Jennifer, Pul, Ümit,, Beijer, Marieke R.,, Egbert J Boekema, Jelle B Bultema, Westra, Edze, Snijders, Ambrosius P.L.,, Lundgren, Magnus, Jennifer A Doudna, Bultema, Jelle B.,, Wagner, R, John van der Oost, Westra, Edze R.,, van der Oost, J, Beijer, M.R., Wurm, R, Beijer, MR, Edze R Westra, Doudna, Jennifer A.,, Kaihong Zhou, Duijn, E., van, Jore, M.M., Heck, AJ, van der Oost, J., Waghmare, Sakharam, Jore, MM, Westra, E.R., Brouns, SJ, Doudna, Jennifer A., Wurm, Reinhild, Wagner, Rolf,, Ambrosius P L Snijders, Zhou, K, Barendregt, Arjan, Albert J R Heck, Brouns, Stan J.J.,, Magnus Lundgren, Pul, Uemit, Boekema, Egbert J.,, van Duijn, Esther, Snijders, Ambrosius P. L., Heck, Albert J. R., Lundgren, M, Pul, Ümit, Zhou, Kaihong,, Oost, John van der,, Blake Wiedenheft, Wiedenheft, Blake,, Wiedenheft, Blake, Waghmare, SP, van der Oost, John, Reinhild Wurm, Stan J J Brouns, Brouns, Stan, Jore, Matthijs M.,, Waghmare, Sakharam P., Bultema, Jelle, Zhou, Kaihong, Dickman, MJ, Lundgren, Magnus,, Barendregt, A, Bultema, JB, Jore, Matthijs, Duijn, Esther van,, Jore, Matthijs M., Lundgren, N.M.J., Ümit Pul, Marieke R Beijer, Wiedenheft, B, van Duijn, E., Heck, Albert J.R.,, Heck, Albert, Bultema, Jelle B., van Duijn, E, Westra, Edze R., Esther van Duijn, Doudna, JA   +101 more
core   +3 more sources

p190A/ARHGAP35 and p190B/ARHGAP5 proteins in endometrial cancer: a novel cancer‐relevant paralog interplay

Molecular Oncology, EarlyView.
This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault, Capucine Heraud, Mélissa Correia de Oliveira, Véronique Neaud, Valérie Valesco, Valérie Prouzet‐Mauleon, Béatrice Turcq, Jean‐William Dupuy, Anne‐Aurélie Raymond, Sabrina Croce, Frédéric Saltel, Valérie Lagrée, Violaine Moreau   +12 more
wiley   +1 more source

CRISPR-Cas immunity leads to a coevolutionary arms race between Streptococcus thermophilus and lytic phage

, 2019
CRISPR-Cas is an adaptive prokaryotic immune system that prevents phage infection. By incorporating phage-derived 'spacer' sequences into CRISPR loci on the host genome, future infections from the same phage genotype can be recognized and the phage ...
J Common (21895061), D Morley (7743389), S van Houte (21852338), ER Westra (21852341)   +3 more
core   +6 more sources