Results 91 to 100 of about 321,595 (358)
Advanced Functional Materials, EarlyView.This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti +3 more
wiley +1 more source
Delivering CRISPR: a review of the challenges and approaches
Drug Delivery, 2018 Gene therapy has long held promise to correct a variety of human diseases and defects. Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the mechanism of the CRISPR-based prokaryotic adaptive immune system (CRISPR ...
Christopher A. Lino +3 more
doaj +1 more source
CRISPR/Cas9 Gene-Editing in Cancer Immunotherapy: Promoting the Present Revolution in Cancer Therapy and Exploring More [PDF]
, 2021 Xuejin Ou +4 more
openalex +1 more source
Advanced Functional Materials, EarlyView.This research presents a novel implantable bio‐battery, GF‐OsG, tailored for diabetic bone repair. GF‐OsG generates microcurrents in high‐glucose conditions to enhance vascularization, shift macrophages to the M2 phenotype, and regulate immune responses.
Nanning Lv +10 more
wiley +1 more source
Rapid one-tube RPA-coupled CRISPR/Cas12a-based RID-MyC assay for the diagnosis of fungal keratitis
Indian Journal of OphthalmologyPurpose: This study introduces and evaluates the single-tube rapid identification of mycoses using CRISPR (ST-RID-MyC) assay. This novel diagnostic tool combines recombinase polymerase amplification (RPA) with CRISPR/Cas12a for the rapid and precise ...
Hanith R Deivarajan +9 more
doaj +1 more source
Surprising CRISPR roadblocks [PDF]
Nature Methods, 2018 Cas9 induces larger-than-anticipated mutations in mouse and human cells. In the latter, efficient editing depends on inhibition of the DNA-damage-repair protein p53.
openaire +2 more sources
The CRISPR tool kit for genome editing and beyond
Nature Communications, 2018 CRISPR is becoming an indispensable tool in biological research. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology ...
Mazhar Adli
semanticscholar +1 more source
4D Mapping of ZIF Biocomposites for High Protein Loading and Tunable Release Profiles
Advanced Functional Materials, EarlyView.Systematic four‐dimensional mapping of zeolitic imidazolate framework biocomposites reveals how precursor ratios, total concentration, and washing define crystalline phase, protein loading, and release kinetics. This comprehensive study identifies conditions yielding record loading (∼85%) and precise phase–property correlations.
Michael R. Hafner +12 more
wiley +1 more source
Guidelines for Performing CRISPR/Cas9 Genome Editing for Gene Validation and Trait Improvement in Crops [PDF]
, 2023 Νικόλαος Τσακιρπάλογλου +2 more
openalex +1 more source
Biochemical Society TransactionsWith the discovery of CRISPR-controlled proteases, CRISPR–Cas has moved beyond mere nucleic acid targeting into the territory of targeted protein cleavage. Here, we review the understanding of Craspase, the best-studied member of the growing CRISPR RNA-guided protease family.
Sam P. B. van Beljouw, Stan J. J. Brouns
openaire +2 more sources