Results 91 to 100 of about 349,258 (340)

Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope

Nature Communications
TnpBs encoded by the IS200/IS605 family transposon are among the most abundant prokaryotic proteins from which type V CRISPR-Cas nucleases may have evolved.
Xu Feng, Ruyi Xu, Jianglan Liao, Jingyu Zhao, Baochang Zhang, Xiaoxiao Xu, Pengpeng Zhao, Xiaoning Wang, Jianyun Yao, Pengxia Wang, Xiaoxue Wang, Wenyuan Han, Qunxin She   +12 more
doaj   +1 more source

GDP‐fucose transporter SLC35C1: a potential regulatory role in cytosolic GDP‐fucose and fucosylated glycan synthesis

FEBS Open Bio, EarlyView.
The inactivation of SLC35C1 (GDP‐fucose transporter) and enzymes involved in GDP‐fucose biosynthesis was studied. Fucose supplementation increases the level of GDP‐fucose to abnormal, millimolar values in the absence of the TSTA3 protein and SLC35C1 in contrast to the GMDS/SLC35C1 double mutant.
Edyta Skurska, Mariusz Olczak
wiley   +1 more source

A CRISPR Test for Rapidly and Sensitively Detecting Circulating EGFR Mutations

Diagnostics, 2020
The detection of EGFR mutations in circulating cell-free DNA can enable personalized therapy for cancer. The current techniques for detecting circulating EGFR mutations are expensive and time-consuming with moderate sensitivity.
Jen-Hui Tsou, Qixin Leng, Feng Jiang
doaj   +1 more source

Identifying prognostic targets in metastatic prostate cancer beyond AR

FEBS Open Bio, EarlyView.
Genome‐wide functional screens combined with a large gene expression database and clinical outcomes can identify new therapeutic vulnerabilities in prostate cancer. Eight potentially druggable targets demonstrated strong dependency in cell lines, were associated with worse prognosis clinically, and showed evidence of protein expression in prostate ...
Emily Feng, Eric Feng, Tracy Berg, Isabella S. Nguyen, Lilac G. Nguyen, William Chen, Meng Zhang, David Quigley, Marina Sharifi, Haolong Li, Ilsa Coleman, Peter S. Nelson, Martin Sjöström, Shuang G. Zhao   +13 more
wiley   +1 more source

Anti-CRISPR Phages Cooperate to Overcome CRISPR-Cas Immunity [PDF]

Cell, 2018
SummarySome phages encode anti-CRISPR (acr) genes, which antagonize bacterial CRISPR-Cas immune systems by binding components of its machinery, but it is less clear how deployment of these acr genes impacts phage replication and epidemiology. Here we demonstrate that bacteria with CRISPR-Cas resistance are still partially immune to Acr-encoding phage ...
Landsberger, Mariann, Gandon, Sylvain, Meaden, Sean, Rollie, Clare, Chevallereau, Anne, Chabas, Hélène, Buckling, Angus, Westra, Edze, van Houte, Stineke   +8 more
openaire   +7 more sources

CNS Mitochondria‐Derived Vesicle in Blood: Potential Biomarkers for Brain Mitochondria Dysfunction

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial dysfunction is a hallmark of neurodegenerative diseases like Alzheimer's (AD) and Parkinson's (PD). Our goal was to develop practical, noninvasive methods to assess mitochondrial status through the detection of mitochondria‐derived vesicles (MDVs).
Qi Liu, Wentao Chen, Yahong Wu, Zhen Guo, Jun Chen, Chen Tian, Pan Wang, Shaopeng Zeng, Bin Xu, Jing Duan, Shilong Han, Xiao Xiong, Jing Zhang   +12 more
wiley   +1 more source

SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing

G3: Genes, Genomes, Genetics, 2020
CRISPR-Cas9 is a powerful genome editing technology in which a single guide RNA (sgRNA) confers target site specificity to achieve Cas9-mediated genome editing.
Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie E. Mohr, Yanhui Hu, Norbert Perrimon   +6 more
doaj   +1 more source

CRISPR-Cas9-mediated functional dissection of 3'-UTRs. [PDF]

, 2017
Many studies using reporter assays have demonstrated that 3' untranslated regions (3'-UTRs) regulate gene expression by controlling mRNA stability and translation.
Ahituv, Nadav, Biton, Anne, Erle, David J, Siegel, David, Tonqueze, Olivier Le, Zaitlen, Noah, Zhao, Wenxue   +6 more
core   +1 more source

The Regulation of Trace Metal Elements in Cancer Ferroptosis

Advanced Biology, EarlyView.
The induction of ferroptosis inhibits tumor growth, enhances anticancer efficacy, and overcomes drug resistance. Recent evidence shows nonferrous metal elements play a role in ferroptosis. This review focuses on how trace metals regulate ferroptosis processes like iron accumulation, lipid peroxidation, and antioxidant defense.
Xiaoyan Wang, Yuanyuan Xue, Lei Chang, Xuena Zhu, Wenjun Liu, Tingbo Liang   +5 more
wiley   +1 more source

Identifying candidate structured RNAs in CRISPR operons

RNA Biology, 2022
Noncoding RNAs with secondary structures play important roles in CRISPR-Cas systems. Many of these structures likely remain undiscovered. We used a large-scale comparative genomics approach to predict 156 novel candidate structured RNAs from 36,111 ...
Brayon J. Fremin, Nikos C. Kyrpides
doaj   +1 more source