Results 121 to 130 of about 333,510 (359)

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]

, 2018
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin, A Burt, A Hammond, AP Lorenzetti, B McClintock, BA Castilho, C Feschotte, C Feschotte, C Feschotte, C Kettlun, C Lu, C Ye, CN Hancock, DBT Cox, DJ Garfinkel, F Palazzoli, FA Ran, FJM Mojica, G Feng, G Mai, G Yang, H Kuang, H Mao, HT Dong, IK Jordan, J Hou, J Li, J Paszkowski, JA Doudna, JB Owens, JD Hollister, JE DiCarlo, JI Qüesta, JK Baillie, JM Casacuberta, K Kikuchi, K Naito, K Naito, K Shirasawa, KD Kim, KM Esvelt, KS Makarova, L Bortesi, L Cong, LC Li, LM Vaschetto, Luis María Vaschetto, M Cowley, M Jinek, M Momose, M Patel, M Szabo, M Turner, N Buchon, N Jiang, N Jiang, N Jiang, Q Zhang, R Barrangou, R Jansen, RM Walsh, SD Molyneux, SR Wessler, SR Yant, T Mourier, T Sakuma, T Singer, VM Gantz, W Matsunaga, WC Lai, X Li, X Li, X Lin, X Shan, Y Ishino, Y Yan, ZN Adelman   +76 more
core   +1 more source

CRISPR application

, 2023
At CRISPR/Cas9 PlatformCB, we provide various custom gene-editing services for customers, including gene knockout, gene knockin and point mutation. With the excellent CRISPR/Cas9 platform and experienced scientists, our well-trained staff successfully finishes scores of custom gene editing projects every year. Our scientists work together and try their
openaire   +2 more sources

Recent Applications of Mesoporous Silica Nanoparticles in Gene Therapy

Advanced Healthcare Materials, EarlyView.
The review summarizes the synthesis of mesoporous silica nanoparticles (MSNs) with modifiable surface properties, functionalization strategies, mechanism of therapeutic payload release, and current applications in gene therapy, focusing on their capabilities in the targeted delivery of therapeutic nucleic acids, CRISPR‐Cas systems, and other genetic ...
Tamanna Binte Huq, Punnya Anil Kumar Jeeja, Sudip Kumar Dam, Sabya Sachi Das, Juan L. Vivero‐Escoto   +4 more
wiley   +1 more source

SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing

G3: Genes, Genomes, Genetics, 2020
CRISPR-Cas9 is a powerful genome editing technology in which a single guide RNA (sgRNA) confers target site specificity to achieve Cas9-mediated genome editing.
Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie E. Mohr, Yanhui Hu, Norbert Perrimon   +6 more
doaj   +1 more source

Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes. [PDF]

, 2020
A number of recent papers report that standing genetic variation in natural populations includes ubiquitous polymorphisms within target sites for Cas9-based gene drive (CGD) and that these "drive resistant alleles" (DRA) preclude the successful ...
Collier, Travis C, Hanemaaijer, Mark J, Houston, Parker D, Lanzaro, Gregory C, Lee, Yoosook, Schmidt, Hanno   +5 more
core   +1 more source

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]

, 2015
Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F, Kaulich, Manuel, Lee, Yeon J, Lönn, Peter, Meade, Bryan R, Springer, Aaron D   +5 more
core   +1 more source

Evolution and Ecology of CRISPR [PDF]

Annual Review of Ecology, Evolution, and Systematics, 2016
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems are prokaryotic adaptive immune systems that provide protection against infection by parasitic mobile genetic elements, such as viruses and plasmids. CRISPR-Cas systems are found in approximately half of all sequenced bacterial genomes and in nearly all ...
Westra, Edze R., Dowling, Andrea J., Broniewski, Jenny M., van Houte, Stineke   +3 more
openaire   +2 more sources

Engineering CAR‐T Therapeutics for Enhanced Solid Tumor Targeting

Advanced Materials, EarlyView.
CART cell therapy has proven effective for blood cancers but struggles with solid tumors due to diverse antigens and complex environments. Recent efforts focus on improving CAR design and validation platforms. Advances in protein engineering, machine learning, and organoid systems aim to enhance CAR‐T therapy against solid tumors.
Danqing Zhu, Won Joon Kim, Hyunjin Lee, Xiaoping Bao, Pilnam Kim   +4 more
wiley   +1 more source

CRISPR for Cryptosporidium [PDF]

Nature, 2015
Study of the diarrhoea-causing pathogen Cryptosporidium has been hindered by a lack of genetic-modification and culture tools. A description of genome editing and propagation methods for the parasite changes this picture. See Letter p.477 The protozoan parasite Cryptosporidium is a major cause of ...
openaire   +3 more sources

Micro‐ and Nano‐Bots for Infection Control

Advanced Materials, EarlyView.
This review presents a strategic vision for integrating micro‐ and nanobots in the pipeline for infection diagnosis, prevention, and treatment. To develop these robots as a practical solution for infection management, their design principles are clarified based on their propulsion mechanisms and then categorized infection management domains based on ...
Azin Rashidy Ahmady, Shadman Khan, Hong Han, Wei Gao, Zeinab Hosseinidoust, Tohid F. Didar   +5 more
wiley   +1 more source