Results 171 to 180 of about 333,510 (359)

Delivery of A Chemically Modified Noncoding RNA Domain Improves Dystrophic Myotube Function

Advanced Science, EarlyView.
CYTOR is a pro‐myogenic ncRNA in skeletal muscle. Here, chemical probing of CYTOR, coupled with functional cellular assays identifies exon 2 as an independent myogenic RNA domain. Chemical engineering of CYTOR exon 2 RNA improves pharmacologic properties, including RNA stability and cell‐autonomous immunogenicity.
Zeinabou Niasse‐Sy, Bo Zhao, Ajda Lenardič, Huyen Thuc Tran Luong, Ori Bar‐Nur, Johan Auwerx, Martin Wohlwend   +6 more
wiley   +1 more source

The on–off switch of CRISPR immunity against phages in Escherichia coli [PDF]

, 2010
Francisco J. M. Mojica, César Díez‐Villaseñor   +1 more
openalex   +1 more source

Copy Number Gains of VPS72 Drive De Novo Lipogenesis and Hepatocarcinogenesis via ATF3/mTORC1/SREBP1 Axis

Advanced Science, EarlyView.
VPS72 overexpression activates mTORC1 signaling, subsequently promoting lipid synthesis and driving HCC progression. VPS72 modulates the epigenetic landscape of ATF3 promoter, such as H2A.Z acetylation reduction and DNA methylation increase, which results in ATF3 repression and subsequent activation of mTORC1.
Qinglin Zhang, Yunxing Huang, Yin Tong, Kenneth Tsz Chun Ng, Jiangwen Zhang   +4 more
wiley   +1 more source

Coordinated Role of Autophagy and ERAD in Maintaining Neuroendocrine Function by Preventing Prohormone Aggregation

Advanced Science, EarlyView.
By studying the maturation mechanisms of vasopressin (AVP), this manuscript identifies FAM134B‐mediated ER‐phagy as a critical pathway for degrading both aggregated proAVP and the key ERAD component, HRD1. HRD1 induction mitigates proAVP aggregation and support AVP neuron function in the absence of autophagy.
Xuya Pan, Xing He, Su Wu, Na Xiong, Xinyu Hou, Heting Wang, Diane Somlo, Martin Spiess, Haiyang Wang, Jifeng Yang, Chunliang Li, Shasha Li, Wenbin Ma, Yanming Chen, Jun Cui, Ling Qi, Guojun Shi   +16 more
wiley   +1 more source

A functional sgRNA-CRISPR screening method for generating murine RET and NTRK1 rearranged oncogenes

Biology Open, 2023
Laura Schubert, Anh T. Le, Trista K. Hinz, Andre C. Navarro, Sarah K. Nelson-Taylor, Raphael A. Nemenoff, Lynn E. Heasley, Robert C. Doebele   +7 more
doaj   +1 more source

Fast Antibiotic resistance-Based gene editing of mammalian cells with CRISPR-Cas9 (FAB-CRISPR) [PDF]

arXiv
Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency causing low knock-in rates. We present a detailed pipeline using HDR donor plasmids containing antibiotic resistance cassettes for rapid selection of gene-edited cells.
arxiv  

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
Matthijs M. Jore, Magnus Lundgren, Esther van Duijn, Jelle B. Bultema, Edze R. Westra, Sakharam Waghmare, Blake Wiedenheft, Ümit Pul, Reinhild Wurm, Rolf Wagner, Marieke R. Beijer, Arjan Barendregt, Kaihong Zhou, Ambrosius P. Snijders, Mark J. Dickman, Jennifer A. Doudna, Egbert J. Boekema, Albert J. R. Heck, John van der Oost, Stan J. J. Brouns   +19 more
openalex   +1 more source

hESCs‐derived Organoids Achieve Liver Zonation Features through LSEC Modulation

Advanced Science, EarlyView.
This article addresses the absence of zonation features in current organoid models by co‐culturing human embryonic stem cell‐derived hepatocytes with liver sinusoidal endothelial cells exhibiting distinct zonal characteristics. It demonstrates that differential WNT2 signaling from endothelial cells modulates hepatocyte zonation and metabolic functions,
Yuying Zhang, Chenyan Huang, Lei Sun, Lyu Zhou, Yudi Niu, Kaini Liang, Bingjie Wu, Peng Zhao, Zhiqiang Liu, Xiaolin Zhou, Peng Zhang, Jianchen Wu, Jie Na, Yanan Du   +13 more
wiley   +1 more source

Structural and Functional Characterization of an Archaeal Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Complex for Antiviral Defense (CASCADE) [PDF]

, 2011
Nathanael G. Lintner, Melina Kerou, Susan K. Brumfield, Shirley Graham, Huanting Liu, James H. Naismith, Matthew A Sdano, Nan Peng, Qunxin She, Valérie Copié, Mark Young, Malcolm F. White, C. Martin Lawrence   +12 more
openalex   +1 more source

Enhancer Profiling Reveals a Protective Role of RXRα Against Calcium Oxalate‐Induced Crystal Deposition and Kidney Injury

Advanced Science, EarlyView.
Comprehensive epigenomic and transcriptomic profiling revealed a dynamic enhancer landscape and gene expression program associated with nephrolithiasis, highlighting RXRα as a central transcription factor in this regulatory network. Tubular‐specific deletion of RXRα increased intrarenal CaOx crystal deposition, while its activation with Bexarotene ...
Yu Yang, Xudan Dou, Yongzhan Sun, Mengyao Wang, Jing Wang, Xinyi Cao, Haijie Xie, Linguo Xie, Weiping Tian, Jing Nie, Yupeng Chen, Chunyu Liu, Lirong Zhang   +12 more
wiley   +1 more source