Results 161 to 170 of about 315,823 (196)

CRISPR/Cas9

2017
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire   +2 more sources

CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics

Angewandte Chemie International Edition, 2023
Abstract Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires a long turnaround time, expensive equipment, and trained personnel, limiting its widespread availability and diagnostic capacity.
Zhengyan Weng, Zheng You, Jie Yang, Noor Mohammad, Mengshi Lin, Qingshan Wei, Xue Gao, Yi Zhang   +7 more
openaire   +2 more sources

Frontiers in CRISPR

ACS Chemical Biology, 2018
CRISPR-based approaches to genetic engineering are progressing at a rapid pace and present exciting new avenues for science, medicine, and technology. Many of the most cutting-edge advances in genome engineering are encompassed in the Research Articles, Reviews, and Perspectives in this special issue, often with an eye toward future directions for the ...
openaire   +2 more sources

Tracking CRISPR’s Footprints

2019
The programmable clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and CRISPR-Cas9-derived gene editing and manipulation tools have revolutionized biomedical research over the past few years. One important category of assisting technologies in CRISPR gene editing is methods used for detecting and quantifying ...
Lin, Lin, Luo, Yonglun; id_orcid 0000-0002-0007-7759   +1 more
openaire   +3 more sources

Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems

Annual Review of Biochemistry, 2020
Clustered regularly interspaced short palindromic repeats (CRISPR) together with their accompanying cas (CRISPR-associated) genes are found frequently in bacteria and archaea, serving to defend against invading foreign DNA, such as viral genomes. CRISPR-Cas systems provide a uniquely powerful
Alan R, Davidson, Wang-Ting, Lu, Sabrina Y, Stanley, Jingrui, Wang, Marios, Mejdani, Chantel N, Trost, Brian T, Hicks, Jooyoung, Lee, Erik J, Sontheimer   +8 more
openaire   +2 more sources

CRISPR technology

Nature Biotechnology, 2022
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An anti-CRISPR that pulls apart a CRISPR–Cas complex

Nature
In biological systems, the activities of macromolecular complexes must sometimes be turned off. Thus, a wide variety of protein inhibitors has evolved for this purpose. These inhibitors function through diverse mechanisms, including steric blocking of crucial interactions, enzymatic modification of key residues or substrates, and perturbation of post ...
Chantel N. Trost, Jing Yang, Bianca Garcia, Yurima Hidalgo-Reyes, Beatrice C. M. Fung, Jiuyu Wang, Wang-Ting Lu, Karen L. Maxwell, Yanli Wang, Alan R. Davidson   +9 more
openaire   +2 more sources

Structural Biology of CRISPR–Cas Immunity and Genome Editing Enzymes

Nature Reviews Microbiology, 2022
Patrick Pausch, Jennifer Doudna, Joy Y. Wang   +2 more
exaly  

Alternative Functions of CRISPR–Cas Systems in the Evolutionary Arms Race

Nature Reviews Microbiology, 2022
John van der Oost, Prarthana Mohanraju, Raymond H J Staals   +2 more
exaly  

Evolutionary Classification of CRISPR–Cas Systems: A Burst of Class 2 and Derived Variants

Nature Reviews Microbiology, 2020
John van der Oost, David A Scott, Omer S Alkhnbashi   +2 more
exaly