Results 311 to 320 of about 321,595 (358)
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Science, 2021
New CRISPR enzyme activities add to the nucleic acid detection ...
Omar O, Abudayyeh +1 more
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New CRISPR enzyme activities add to the nucleic acid detection ...
Omar O, Abudayyeh +1 more
openaire +2 more sources
2023
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +2 more sources
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +2 more sources
CRISPR Critters and CRISPR Cracks
The American Journal of Bioethics, 2015This essay focuses on possible nonhuman applications of CRISPR/Cas9 that are likely to be widely overlooked because they are unexpected and, in some cases, perhaps even "frivolous." We look at five uses for "CRISPR Critters": wild de-extinction, domestic de-extinction, personal whim, art, and novel forms of disease prevention.
R Alta, Charo, Henry T, Greely
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CRISPR technology: A decade of genome editing is only the beginning
Science, 2023The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome editing, coupled with advances in computing and imaging capabilities, has initiated a new era in which genetic diseases and individual disease susceptibilities are ...
Joy Y. Wang, J. Doudna
semanticscholar +1 more source
An updated evolutionary classification of CRISPR–Cas systems
Nature Reviews Microbiology, 2015 Kira S Makarova +2 more
exaly +2 more sources
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Nature, 2014 Silvana Konermann +2 more
exaly +2 more sources
2017
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +2 more sources
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +2 more sources
Expert Opinion on Drug Discovery, 2021
Introduction: The ability to engineer mammalian genomes in a quick and cost-effective way has led to rapid adaptation of CRISPR technology in biomedical research. CRISPR-based engineering has the potential to accelerate drug discovery, to support the reduction of high attrition rate in drug development and to enhance development of cell and gene-based ...
Laure Grand Moursel +4 more
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Introduction: The ability to engineer mammalian genomes in a quick and cost-effective way has led to rapid adaptation of CRISPR technology in biomedical research. CRISPR-based engineering has the potential to accelerate drug discovery, to support the reduction of high attrition rate in drug development and to enhance development of cell and gene-based ...
Laure Grand Moursel +4 more
openaire +2 more sources
Nature Microbiology, 2023
CRISPR systems canonically confer microorganisms with protection against invading viral DNA, plasmids and mobile genetic elements, however a multi-omics investigation of deep subsurface archaeal communities suggests that archaeal CRISPR systems might target other archaeal parasites or force a transition from parasitism to mutualism.
Anna-Louise Reysenbach, Michael P. Terns
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CRISPR systems canonically confer microorganisms with protection against invading viral DNA, plasmids and mobile genetic elements, however a multi-omics investigation of deep subsurface archaeal communities suggests that archaeal CRISPR systems might target other archaeal parasites or force a transition from parasitism to mutualism.
Anna-Louise Reysenbach, Michael P. Terns
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Tracking CRISPR’s Footprints
2019The programmable clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and CRISPR-Cas9-derived gene editing and manipulation tools have revolutionized biomedical research over the past few years. One important category of assisting technologies in CRISPR gene editing is methods used for detecting and quantifying ...
Lin, Lin, Luo, Yonglun
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