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CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics
Angewandte Chemie International Edition, 2023AbstractPolymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires a long turnaround time, expensive equipment, and trained personnel, limiting its widespread availability and diagnostic capacity.
Zhengyan Weng +7 more
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2017
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
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CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +2 more sources
Nature Methods, 2013
An enzymatically dead Cas9 endonuclease permits targeted, RNA-guided transcriptional silencing.
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An enzymatically dead Cas9 endonuclease permits targeted, RNA-guided transcriptional silencing.
openaire +3 more sources
2022
El nanoruler GATTA-PAINT 40 RG (GATTAquant, DNA nanotechnologies) consta de tres fluoróforos (ATTO 655/ATTO 542) separados 40 nm entre sí. La obtención de imágenes de la muestra se realizó en un microscopio invertido Olympus IX-81 equipado con un objetivo de inmersión en aceite Olympus UApo N 100X/1.49 NA en modo de iluminación TIRF (Olympus, cellTIRF ...
openaire +1 more source
El nanoruler GATTA-PAINT 40 RG (GATTAquant, DNA nanotechnologies) consta de tres fluoróforos (ATTO 655/ATTO 542) separados 40 nm entre sí. La obtención de imágenes de la muestra se realizó en un microscopio invertido Olympus IX-81 equipado con un objetivo de inmersión en aceite Olympus UApo N 100X/1.49 NA en modo de iluminación TIRF (Olympus, cellTIRF ...
openaire +1 more source
2019
The programmable clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and CRISPR-Cas9-derived gene editing and manipulation tools have revolutionized biomedical research over the past few years. One important category of assisting technologies in CRISPR gene editing is methods used for detecting and quantifying ...
Lin, Lin, Luo, Yonglun
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The programmable clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and CRISPR-Cas9-derived gene editing and manipulation tools have revolutionized biomedical research over the past few years. One important category of assisting technologies in CRISPR gene editing is methods used for detecting and quantifying ...
Lin, Lin, Luo, Yonglun
openaire +4 more sources
CRISPR and Salty: CRISPR-Cas Systems in Haloarchaea
2017CRISPR-Cas (CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats and Cas: CRISPR associated) systems are unique defence mechanisms since they are able to adapt to new invaders and are heritable. CRISPR-Cas systems facilitate the sequence-specific elimination of invading genetic elements in prokaryotes, they are found in 45% of bacteria and
Lisa-Katharina Maier +3 more
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