Results 71 to 80 of about 15,117,110 (236)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

A weight function-critical plane approach for low-cycle fatigue under variable amplitude multiaxial loading

, 2019
Low-cycle fatigue data of type 304 stainless steel obtained under axial-torsional loading of variable amplitudes are analyzed using four multiaxial fatigue parameters: SWT, KBM, FS and LKN.
Jin, D, Chen, X, Kim, KS
core   +1 more source

Fatigue assessment of a FSAE car rear upright by a closed form solution of the critical plane method

Fracture and Structural Integrity
Material fatigue is extensively discussed and researched within scientific and industrial communities. Fatigue damage poses a significant challenge for both metallic and non-metallic components, often resulting in unexpected failures of in-service parts.
A. Chiocca, M. Sgamma, F. Frendo, F. Bucchi, G. Marulo   +4 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Multiaxial Fatigue Lifetime Estimation Based on New Equivalent Strain Energy Damage Model under Variable Amplitude Loading

Crystals
The largest normal stress excursion during contiguous turn time instants of the maximum torsional stress is presented as an innovative path-independent fatigue damage quantity upon the critical plane, which is further employed for characterizing fatigue ...
Zhi-Qiang Tao, Xiangnan Pan, Zi-Ling Zhang, Hong Chen, Li-Xia Li   +4 more
doaj   +1 more source

Energy Concepts and Critical Plane for Fatigue Assessment of Ti-6Al-4V Notched Specimens

Applied Sciences, 2019
In the present paper, the fatigue life assessment of notched structural components is performed by applying a critical plane-based multiaxial fatigue criterion.
Camilla Ronchei, Andrea Carpinteri, Sabrina Vantadori   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Lost and Finding: Experiences of Newly Graduated Critical Social Workers [PDF]

, 2019
The first year of social work practice can be very stressful for new practitioners. Practice develops in response to the meaning we place on what we do and on our professional goals.

core  

Multiaxial Fatigue Life Calculation Model for Engineering Components in Rolling-Sliding Contact

MATEC Web of Conferences, 2018
Number of important engineering components and elements such as gears, rollers, bearings operate in conditions of rolling-sliding contact loading. Determination of fatigue lives of such components and elements is very important for engineering practice ...
Basan Robert, Marohnić Tea
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source