Results 131 to 140 of about 25,486,006 (176)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source
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Critical technocultural discourse analysis

New Media & Society, 2016
Critical Technocultural Discourse Analysis (CTDA) is a multimodal analytic technique for the investigation of Internet and digital phenomena, artifacts, and culture. It integrates an analysis of the technological artifact and user discourse, framed by cultural theory, to unpack semiotic and material connections between form, function, belief, and ...
openaire   +1 more source

Critical Discourse Analysis

Annual Review of Applied Linguistics, 1990
The label Critical Discourse Analysis (CDA) is used by a significant number of scholars with a diverse set of concerns in a number of disciplines. It is well-exemplified by the editorial statement of the journal Discourse and Society, which defines its envisaged domain of enquiry as follows: “the reproduction of sexism and racism through discourse; the
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Critical Discourse Analysis

Annual Review of Anthropology, 2000
▪ Abstract  This paper provides a survey of critical discourse analysis (CDA), a recent school of discourse analysis that concerns itself with relations of power and inequality in language. CDA explicitly intends to incorporate social-theoretical insights into discourse analysis and advocates social commitment and interventionism in research.
Jan Blommaert, Chris Bulcaen
openaire   +1 more source

Critical Discourse Analysis

2020
Critical discourse analysis (CDA) is a social scientific theory and method for analyzing and critiquing the use of language and its contribution to forming and sustaining social practice and for analysis of how language can contribute to reproducing or transforming social problems.
openaire   +3 more sources

Discourse Analysis, Critical Discourse Analysis, and Conversation Analysis

2019
This chapter examines discourse analysis writ large as a catalog of methods by which writing center researchers can examine talk and text. They are discourse analysis (DA), critical discourse analysis (CDA), and conversation analysis (CA). Each of these is discussed as theory and method, with particular attention to how intersubjectivity is constructed.
openaire   +1 more source

Critical Discourse Analysis /Critical Discourse Studies

Abstract The article provides a state of the art overview of Critical Discourse Analysis, more recently Critical Discourse Studies (here referred to as CDA/S). CDA/S is an evolving, interdisciplinary and transdisciplinary multi-methods field of inquiry concerned with the study of the relationship between language and power. The article defines the core
openaire   +2 more sources

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