Results 161 to 170 of about 1,563,368 (312)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Identification of secondary flow pattern in a heated curved rectangular channel using image processing technique

, 2001
This paper introduced a new application of pattern recognition technique to the problem of automatic identification of secondary flow patterns in a heated curve rectangular channels. The proposed algorithm is based on two observations.
Chandratilleke, T., Eren, H., Fung, C.C., Chung, K.P.   +3 more
core  

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Critical flows and plants

, 2012
In this physics PHD thesis applied to vegetal physiology we focus on two phenomenons dealing with plants. The capillary ascension of liquids in corners and lodging of cereals and trees. When plants are under high hydraulic stress, we observe gas bubbles appearing in their sap, this is gaseous embolism of plants. Hence, there are situations with sap/air
openaire   +1 more source

Tissue microvascular flow and oxygenation in critically ill patients

, 2012
PhDThe use of fluid resuscitation and vasoactive agents to optimise global haemodynamics has been demonstrated to improve outcomes in patients undergoing major surgery and in early sepsis.
Jhanji, Shaman
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Cardiac Arrest as the First Manifestation of Single Coronary Artery-Potential Role of FFR_CT for Detecting Critical Flow Reduction. A Case Report. [PDF]

Clin Case Rep
Nehaj F, Hatala R, Kocan D, Collet C, Hlivak P, Svetlosak M, Ali U, Connolly DL.   +7 more
europepmc   +1 more source

Water two-phase flow through pressure safety valve with variable backpressure: check of calculation methods to estimate mass flow-rate and critical flow conditions

, 2003
A careful design of pressure safety valves (PSV) is an essential requirement for safeguarding of industrial plants; reliable correlations are available for PSV design in the cases of liquid or gas discharge.
M. Cumo, G. Boccardi, F. Di Tosto, BUBBICO, Roberto, G. P. Celata   +4 more
core