Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Transportation Requirements for Projected Grain and Oilseed Crop Production to 1999-2000 for North Dakota [PDF]
Projections of crop production and livestock consumption of selected grains and oilseed crops reported in this publication were prepared as part of a North Central (NC) Regional Research Committee 137 effort.
Cobia, David W.
core
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
SIMULATION OF SOIL WATER-CROP YIELD SYSTEMS: THE POTENTIAL FOR ECONOMIC ANALYSIS [PDF]
Crop Production/Industries,
Eidman, Vernon R., Mapp, Harry P., Jr.
core
Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells
FEBS Open Bio, EarlyView.Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim +4 more
wiley +1 more source
Prediction of soybean yellow mottle mosaic virus in soybean using hyperspectral imaging
Plant MethodsDisease incidence is a key factor contributing to reduced crop yield. Thus, early identification of crop diseases is crucial for minimizing the effects of disease incidence and maximizing crop yield.
Amit Ghimire +3 more
doaj +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Crop Revenue and Yield Insurance Demand: A Subjective Probability Approach [PDF]
A multinomial logit is utilized to model the choice of whether to purchase yield or revenue insurance using subjectively elicited survey data. Our results indicate that the demand for crop insurance is inelastic (-0.40), consistent with most earlier ...
Coble, Keith H. +4 more
core
Discover SoilChicken manure soil amendment is an integral part of crop production that supplies nutrients for optimum crop growth but increases potentially toxic element (PTE) contaminants in soil and harvested crop.
Oladele Abdulahi Oguntade +6 more
doaj +1 more source
Optimization of Genetic Transformation System in Brassica napus via Agrobacterium
Journal of Applied Research in Plant SciencesEfficient callus induction and plant regeneration are critical steps in plant tissue culture and genetic transformation. This study focused on optimizing nutrient media, plant growth regulators (PGRs), sterilization protocols, and transformation ...
Faizan Imtiaz +5 more
doaj +1 more source