Results 1 to 10 of about 95,368 (166)
Insights into Transient Dimerisation of Carnitine/Acylcarnitine Carrier (SLC25A20) from Sarkosyl/PAGE, Cross-Linking Reagents, and Comparative Modelling Analysis [PDF]
The carnitine/acylcarnitine carrier (CAC) is a crucial protein for cellular energy metabolism, facilitating the exchange of acylcarnitines and free carnitine across the mitochondrial membrane, thereby enabling fatty acid β-oxidation and oxidative ...
Nicola Giangregorio +3 more
doaj +2 more sources
PhoX: An IMAC-Enrichable Cross-Linking Reagent [PDF]
Abstract Chemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry.
Richard Scheltema +2 more
exaly +6 more sources
Generally, gelatin was irreversibly cross-linked by chemical reagents to improve its water-resistance. However, few chemical reagents meet both the requirements of high cross-inking efficiency and tunable degradation.
Shengbin He +7 more
doaj +1 more source
Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition.
Mayya V. Monakhova +9 more
doaj +1 more source
Cross-linking mass spectrometry is widely used to elucidate protein structures and interactions. Here, the authors generate an extensive peptide library to benchmark the most common cross-link search engines with frequently used cross-linking reagents in
Manuel Matzinger +5 more
doaj +1 more source
Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review
The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability
Alexandra Virginia Bounegru +1 more
doaj +1 more source
Ligand-induced movements of inner transmembrane helices of Glut1 revealed by chemical cross-linking of di-cysteine mutants. [PDF]
The relative orientation and proximity of the pseudo-symmetrical inner transmembrane helical pairs 5/8 and 2/11 of Glut1 were analyzed by chemical cross-linking of di-cysteine mutants.
Mike Mueckler, Carol Makepeace
doaj +1 more source
Application of proanthocyanidin in the treatment and repair of deep caries
Proanthocyanidin (PA), as a kind of natural plant polyphenol, have a variety of biological functions, such as promoting remineralization, inducing collagen cross-linking, inhibiting protease activity and inhibiting bacteria.
GAO Ying +7 more
doaj +1 more source
ECL 3.0: a sensitive peptide identification tool for cross-linking mass spectrometry data analysis
Background Cross-linking mass spectrometry (XL-MS) is a powerful technique for detecting protein–protein interactions (PPIs) and modeling protein structures in a high-throughput manner.
Chen Zhou +6 more
doaj +1 more source
Covalent cross‐linking of proteins without chemical reagents [PDF]
AbstractA facile method for the formation of zero‐length covalent cross‐links between protein molecules in the lyophilized state without the use of chemical reagents has been developed. The cross‐linking process is performed by simply sealing lyophilized protein under vacuum in a glass vessel and heating at 85°C for 24 h.
Brigitte L, Simons +4 more
openaire +2 more sources

