Results 11 to 20 of about 183,904 (311)

Covalent cross‐linking of proteins without chemical reagents [PDF]

Protein Science, 2002
AbstractA facile method for the formation of zero‐length covalent cross‐links between protein molecules in the lyophilized state without the use of chemical reagents has been developed. The cross‐linking process is performed by simply sealing lyophilized protein under vacuum in a glass vessel and heating at 85°C for 24 h.
Brigitte L, Simons, Mary C, King, Terry, Cyr, Mary Alice, Hefford, Harvey, Kaplan   +4 more
openaire   +2 more sources

Cross-linking of dermal sheep collagen using a water-soluble carbodiimide [PDF]

, 1996
A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC).
Dijkstra, P.J., Feijen, J., Luyn, M.J.A. van, Nieuwenhuis, P., Olde Damink, L.H.H., Wachem, P.B. van   +5 more
core   +8 more sources

PhoX – an IMAC-enrichable Crosslinking Reagent [PDF]

ACS Central Science, 2019
AbstractChemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry.
Steigenberger, Barbara A., Pieters, Roland J., Heck, Albert J.R., Scheltema, Richard A.   +3 more
openaire   +5 more sources

The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents [PDF]

, 1990
Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V. Kurzchalia, E. Hartmarm, and T. A. Rapoport. 1987. Nature
Dobberstein, Bernhard, Görlich, Dirk, Hartmann, Enno, Herz, Joachim, Knespel, Siegne, Kraft, Regine, Otto, Albrecht, Prehn, Siegfried, Rapoport, Tom A., Wiedmann, Martin   +9 more
core   +1 more source

XPS Characterization of Friedel-Crafts Cross-Linked Polystyrene [PDF]

, 2001
The combination of a difunctional alkylating agent, either hydroxymethylbenzyl chloride or α,α′-dichloroxylene with polystyrene or high-impact polystyrene together with a Friedel-Crafts catalyst, 2-ethylhexyldiphenylphosphate, and an amine to react with ...
Du, Jianxin, Wang, Jiangqi, Wilkie, Charles A., Yao, Hongyang   +3 more
core   +2 more sources

Transmembrane helical interactions in the CFTR channel pore.

PLoS Computational Biology, 2017
Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene affect CFTR protein biogenesis or its function as a chloride channel, resulting in dysregulation of epithelial fluid transport in the lung, pancreas and other organs in ...
Jhuma Das, Andrei A Aleksandrov, Liying Cui, Lihua He, John R Riordan, Nikolay V Dokholyan   +5 more
doaj   +1 more source

INVESTIGATIONS ON THE EFFECT OF SODIUM TRIMETAPHOSPHATE AND SODIUM SULFATE CONCENTRATIONS ON THE PROPERTIES OF CROSS-LINKED CASSAVA STARCH FILMS [PDF]

Química Nova
Films based on pure native starch have limited applications, are easily breakable, very hygroscopic, and permeable to water vapor. One method for improving these properties is to modify starch by cross-linking.
Marisa Ferreira Karow, Karine Laste Macagnan, Mariane Igansi Alves, Júlia Borin Fioravante, Rosane da Silva Rodrigues, Lígia Furlan, João Felipe Colosio de Souza, Claire Tondo Vendruscolo, Angelita da Silveira Moreira   +8 more
doaj   +1 more source

Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions [PDF]

, 2012
We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs).
Agnew, Heather D., Beauchamp, J. L., Czerwieniec, Gregg, Deshaies, Raymond J., Graham, Robert L. J., Heath, James R., Hess, Sonja, Lee, J. Eugene, Loo, Joseph A., Smith, Geoffrey T., Sohn, Chang Ho, Sweredoski, Michael J.   +11 more
core   +2 more sources

New immunolatex spheres: visual markers of antigens on lymphocytes for scanning electron microscopy [PDF]

, 1975
New immunochemical reagents consisting of antibodies bound to small latex spheres were used as visual markers for the detection and localization of cell surface antigens by scanning electron microscopy.
Dreyer, William J., Molday, Robert S., Rembaum, Alan, Yen, S. P. S.   +3 more
core   +2 more sources

Mass Spectrometry Evidence for Cisplatin As a Protein Cross-Linking Reagent [PDF]

Analytical Chemistry, 2011
Cisplatin is a potent anticancer drug, which functions by cross-linking adjacent DNA guanine residues. However within 1 day of injection, 65-98% of the platinum in the blood plasma is protein-bound. It is generally accepted that cisplatin binds to methionine and histidine residues, but what is often underappreciated is that platinum from cisplatin has ...
Huilin Li, Yao Zhao, Hazel I. A. Phillips, Yulin Qi, Tzu-Yung Lin, Peter J. Sadler, Peter B. O’Connor   +6 more
openaire   +3 more sources