An integrated method of isolating napin and cruciferin from defatted canola meal
Abstract Canola proteins are known to have great potential for use in food and non-food applications due to their nutritional, biological and functional properties. However, canola protein extraction remains a challenge, due mainly to the complexity of canola proteins and the presence a variety of undesirable compounds.
Jianping Wu
exaly +2 more sources
CRISPR/Cas9 editing of three CRUCIFERIN C homoeologues alters the seed protein profile in Camelina sativa [PDF]
Background The oilseed Camelina sativa is grown for a range of applications, including for biofuel, biolubricants, and as a source of omega-3 fatty acids for the aquaculture feed industry.
Wendy J. Lyzenga +5 more
doaj +3 more sources
Interaction of cruciferin-based nanoparticles with Caco-2 cells and Caco-2/HT29-MTX co-cultures
The objective of this work was to assess the potential of Cruciferin/Calcium (Cru/Ca) and Cruciferin/Chitosan (Cru/Cs) nanoparticles for oral drug delivery. For this purpose, Cru/Ca and Cru/Cs nanoparticles were developed through cold gelation of Cruciferin, a major canola protein, and in interaction with calcium and chitosan, respectively.
Afsaneh Lavasanifar, Jianping Wu
exaly +3 more sources
Coherent multiple scattering in small-angle scattering experiments: modeling approximations based on the Born expansion. [PDF]
A simple approximative approach is presented to model ultra small‐angle neutron scattering and ultra small‐angle X‐ray scattering data when coherent multiple scattering is present. The underlying principles, exact solutions – similar to Mie scattering – and approximations are presented.We start from an analytical formulation for the coherent multiple ...
Frielinghaus H, Gommes CJ.
europepmc +2 more sources
Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review. [PDF]
Global protein consumption is increasing exponentially, which requires efficient identification of potential, healthy, and simple protein sources to fulfil the demands. The existing sources of animal proteins are high in fat and low in fiber composition,
Manikandan A +9 more
europepmc +3 more sources
Seed longevity is modulated by multiple genetic factors in Arabidopsis thaliana. A previous genome-wide association study using the Elevated Partial Pressure of Oxygen (EPPO) aging assay pinpointed a genetic locus associated with this trait.
Regina Niñoles +8 more
doaj +1 more source
Seed-produced anti-globulin VHH-Fc antibodies retrieve globulin precursors in the insoluble fraction and modulate the Arabidopsis thaliana seed subcellular morphology [PDF]
Key message Nanobody-heavy chain (VHH-Fc) antibody formats have the potential to immunomodulate even highly accumulating proteins and provide a valuable tool to experimentally modulate the subcellular distribution of seed storage proteins.
Altmann, Friedrich +7 more
core +2 more sources
The Native Structure and Composition of the Cruciferin Complex in Brassica napus [PDF]
Globulins are an important group of seed storage proteins in dicotyledonous plants. They are synthesized during seed development, assembled into very compact protein complexes, and finally stored in protein storage vacuoles (PSVs). Here, we report a proteomic investigation on the native composition and structure of cruciferin, the 12 S globulin of ...
Nietzel, Thomas +7 more
openaire +3 more sources
Distribution of Napin and Cruciferin in Developing Rape Seed Embryos [PDF]
The distribution of napin and cruciferin, the two major storage proteins in rape seed, Brassica napus, has been visualized during seed development by antibody staining of paraffin-embedded and sectioned seeds. The results indicate that the synthesis of both proteins during embryogenesis is strictly regulated with respect to time and tissue.
A S, Höglund +3 more
openaire +2 more sources
Proteins make up a large percentage of the Brassica seed and are second only to the oil in economic importance with uses for both animal and human nutrition.
Mahmudur Rahman +6 more
doaj +1 more source

