Results 51 to 60 of about 3,860,140 (300)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Blastocystis sp. : Evaluation of polyclonal antibody prepared from crude protein for serological diagnosis using Rabbit serum

Aceh Journal of Animal Science, 2020
The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the ...
Septian Hakim Susantoputro, Lucia Tri Suwanti, Rahadju Ernawati, Mufasirin Mufasirin, Setiawan Koesdarto, Wiwiek Tyasningsih, Heni Puspitasari   +6 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Partner, Kristal and Dukat: A new generation of the Novi Sad spring protein pea (Pisum sativum) cultivars [PDF]

Selekcija i Semenarstvo, 2009
In 2007 and 2008, the trials of the Department of Variety Protection and Registration of the Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia were carried out on four locations, including three new Novi Sad spring pea line,
Mihailović Vojislav, Mikić Aleksandar, Katić Slobodan, Karagić Đura, Milošević Branko, Pataki Imre, Radojević Veljko   +6 more
doaj  

Biochemical Assessment of Peas Pisum sativum Varieties

Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ, 2021
Seven peas (Pisum sativum L.) varieties different originated, i.e, X07P54, X06PWY, NDPO80138-B-2, CA1P, L020140, Wt6803 and Mir were studied. Biochemical assessment of fresh aboveground biomass and seeds of peas varieties were done.
Viliana Vasileva, Valentin Kosev
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

KANDUNGAN PROTEIN KASAR DAN SERAT KASAR TONGKOL JAGUNG YANG DIFERMENTASI DENGAN MENGGUNAKAN TINGKAT CAIRAN RUMEN KERBAU YANG BERBEDA

Ziraa'ah: Majalah Ilmiah Pertanian, 2018
This study based on analyzing the crude protein and crude fibre on the fermented corn cobs using deferent level of buffalo rumen fluid. Research compiled based on experiment with Complete Random Design (CRD) with 5 treatments and 4 replications namely T0
Neni Widaningsih, Siti Dharmawati, Novi Puspitasari   +2 more
doaj   +1 more source

Fractal scaling in crude oil price evolution via Time Series Analysis (TSA) of historical data [PDF]

, 2009
This paper presents historical price data for two different crude oil types and examines the stationarity and inherent structure in oil price variation, applying many degrees of time resolution.
Gerogiorgis, Dimitrios I
core   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source