Results 131 to 140 of about 6,053 (258)

Unraveling atomic complexity from frozen samples

Structural Dynamics
Cryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and microcrystal ...
Yasmeen N. Ruma, Brent L. Nannenga, Tamir Gonen   +2 more
doaj   +1 more source

Discovery of a Potent Fluorescence Polarization Probe for Identifying USP1 Allosteric Inhibitors

Advanced Science, EarlyView.
This study presents the first ubiquitin‐specific protease 1 (USP1) allosteric fluoroprobe and fluorescence polarization assay, enabling the differentiation of allosteric and catalytic site inhibitors. Further, a novel class of tetrahydroisoquinoline‐based USP1 inhibitors is designed, with compound 14a (USP1 IC50 = 29.9 nM) showing strong selectivity ...
Jiawei Cheng, Peipei Wang, Pengfei Wang, Jiamin Wang, Baiyang Li, Daqing Fang, Jian He, Xiaobei Hu, Weijuan Kan, Yubo Zhou, Chunpu Li, Jia Li, Hong Liu   +12 more
wiley   +1 more source

Precipitation‐Modulated Harmonic Architectures Enable Superior Strength–Ductility Synergy from Cryogenic to Elevated Temperatures in Nanostructured Alloys

Advanced Science, EarlyView.
Precipitation‐modulated recrystallization enables a programmable bimodal harmonic architecture in a high‐entropy alloy, delivering 1–2 GPa yield strength with >10% ductility from −196 °C to 700 °C. The resulting broad‐temperature robustness arises from the synergy of dual‐mode nanoprecipitation, harmonic core–shell topology, and temperature‐adaptive ...
Wei Li, Liangyin Xiong, Mengchao Niu, Junhua Luan, Wei Wang, Zengbao Jiao   +5 more
wiley   +1 more source

Cryo–electron microscopy structure and analysis of the P-Rex1–Gβγ signaling scaffold

, 2019
Jennifer N. Cash, Sarah Urata, Sheng Li, Sandeep K. Ravala, Larisa Avramova, Michael Shost, J. Silvio Gutkind, J.J.G. Tesmer, Michael A. Cianfrocco   +8 more
openalex   +1 more source

Fluorescence-Based Detection of Fusion State on a Cryo-EM Grid using Correlated Cryo-Fluorescence and Cryo-Electron Microscopy [PDF]

, 2018
Lauren Ann Metskas, John A. G. Briggs
openalex   +1 more source

Cryo-Electron Microscopy & Reconstruction [PDF]

Biophysical Journal, 2008
openaire   +1 more source

Large-volume cryoEM sample preparation for the investigation of the plant-microbiome interaction [PDF]

BIO Web of Conferences
Liedtke Janine, Moravcová Jana, Křepelka Pavel, van Son Marije, Nováček Jirka, Kokkoris Vasilis, Shimizu Thomas S., Kiers Toby, Briegel Ariane   +8 more
doaj   +1 more source

Scalable Engineering of Bio‐Manufactured Extracellular Vesicles for Selective Delivery in Ovarian Cancer Patient‐Derived Models

Advanced Science, EarlyView.
Engineered extracellular vesicles displaying Ephrin‐B2 selectively target Ephrin‐B4–expressing ovarian cancer cells, enabling precise delivery in patient‐derived models. This scalable bio‐manufacturing platform reveals a versatile strategy to exploit Ephrin signaling for highly specific therapeutic payload delivery and motivates exploration of tailored
Nihar Godbole, Andrew Lai, Alexander Quinn, Marianne Gillard, Dominic Guanzon, Katherin Scholz‐Romero, Alexis Salas‐Burgos, Bastián Lillo Dapremont, Rohan Lourie, Naven Chetty, Richard Lobb, Kate Beecher, Amy E. McCart Reed, Kaltin Ferguson, Biao Sun, Yaowu He, John D. Hooper, Carlos Salomon   +17 more
wiley   +1 more source

A New Customizable Surfactant LLPS Strategy for Sustainable and Highly Efficient Radioactive Metal Ion Separation

Advanced Science, EarlyView.
A diluent‐free, customizable liquid–liquid phase separation (LLPS) platform enables highly efficient radioactive metal ion extraction and stripping. By encapsulating hydrophobic extractants into an ionic surfactant‐rich condensed phase, this LLPS system leverages synergistic electrostatic and coordination interactions.
Ruihan Yan, Yifu Hu, Wentao Wang, Zhi Cao, Meiwen Cao, Weifang Zheng, Guoan Ye, Taihong Yan   +7 more
wiley   +1 more source

Microsecond time-resolved cryo-electron microscopy

Current Opinion in Structural Biology
Microsecond time-resolved cryo-electron microscopy has emerged as a novel approach for directly observing proteins dynamics. By providing microsecond temporal and near-atomic spatial resolution, it has the potential to elucidate a wide range of dynamics that were previously inaccessible and therefore, to significantly advance our understanding of ...
openaire   +3 more sources