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High-Resolution Structures of Tobacco Mosaic Virus Disks from Cryo-Electron Microscopy
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Cryo-Electron Microscopy of Biological Samples
Ultrastructural Pathology, 2006A brief summary of current cryo-electron microscopy methods for processing and imaging biological tissues is provided. The main emphasis is given to two preparation procedures: frozen-hydrated samples because of the remarkable success of cryo-electron crystallography in obtaining near atomic resolution of integral membrane proteins, and high-pressure ...
Martin Costello
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Automated cryo-electron microscopy
Proceedings IEEE International Symposium on Biomedical Imaging, 2003Cryo-electron microscopy is widely viewed as a uniquely powerful method for the study of membrane proteins and large macromolecular complexes - subjects that are viewed as extremely challenging or impossible to study using x-ray or NMR methods. Although the methodology of molecular microscopy has enormous potential, it is time consuming and labor ...
Clinton S. Potter +6 more
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Ultramicroscopy, 1982
Abstract A brief review of significant attempts to overcome the problem of radiation damage is given, which indicates cryo electron microscopy as a likely solution. The need for clear understanding of the physical mechanism of cryo electron microscopy is stressed, and possible means to achieve such an understanding is indicated.
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Abstract A brief review of significant attempts to overcome the problem of radiation damage is given, which indicates cryo electron microscopy as a likely solution. The need for clear understanding of the physical mechanism of cryo electron microscopy is stressed, and possible means to achieve such an understanding is indicated.
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Cryo‐electron microscopy and cryo‐electron tomography of nanoparticles
WIREs Nanomedicine and Nanobiotechnology, 2016Cryo‐transmission electron microscopy (cryo‐TEM or cryo‐EM) and cryo‐electron tomography (cryo‐ET) offer robust and powerful ways to visualize nanoparticles. These techniques involve imaging of the sample in a frozen‐hydrated state, allowing visualization of nanoparticles essentially as they exist in solution. Cryo‐TEM grid preparation can be performed
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Cryo-electron Microscopy of the Giant Mimivirus
Journal of Molecular Biology, 2005Mimivirus is the largest known virus. Using cryo-electron microscopy, the virus was shown to be icosahedral, covered by long fibers, and appears to have at least two lipid membranes within its protein capsid. A unique vertex, presumably for attachment and infection of the host, can be seen for particles that have a suitable orientation on the ...
Chuan, Xiao +6 more
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Cryo-electron Microscopy of Protein Cages
2023Protein cages are one of the most widely studied objects in the field of cryogenic electron microscopy-encompassing natural and synthetic constructs, from enzymes assisting protein folding such as chaperonin to virus capsids. Tremendous diversity of morphology and function is demonstrated by the structure and role of proteins, some of which are nearly ...
Raymond N, Burton-Smith +1 more
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Brain and nerve = Shinkei kenkyu no shinpo, 2019
Cryo-electron microscopy (cryo-EM) includes three technical methods, (1) rapid freezing for vitreous ice-embedding, (2) observation of frozen hydrated specimens, and (3) image processing for three-dimensional structural analysis. The three-dimensional structural analysis can be performed in three different ways.
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Cryo-electron microscopy (cryo-EM) includes three technical methods, (1) rapid freezing for vitreous ice-embedding, (2) observation of frozen hydrated specimens, and (3) image processing for three-dimensional structural analysis. The three-dimensional structural analysis can be performed in three different ways.
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Conventional Electron Microscopy, Cryo-Electron Microscopy and Cryo-Electron Tomography of Viruses
2013Electron microscopy (EM) techniques have been crucial for understanding the structure of biological specimens such as cells, tissues and macromolecular assemblies. Viruses and related viral assemblies are ideal targets for structural studies that help to define essential biological functions.
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Cryo-electron microscopy in color
Nature MethodsCryo-electron microscopy with energy resolution, using the EELS-STEM method, allows researchers to identify the approximate locations of certain heavier atoms within single frozen, hydrated protein particles.
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