Results 251 to 260 of about 2,439,094 (286)

[Cryo-Electron Microscopy].

Brain and nerve = Shinkei kenkyu no shinpo, 2019
Cryo-electron microscopy (cryo-EM) includes three technical methods, (1) rapid freezing for vitreous ice-embedding, (2) observation of frozen hydrated specimens, and (3) image processing for three-dimensional structural analysis. The three-dimensional structural analysis can be performed in three different ways.
openaire   +2 more sources

Conventional Electron Microscopy, Cryo-Electron Microscopy and Cryo-Electron Tomography of Viruses

2013
Electron microscopy (EM) techniques have been crucial for understanding the structure of biological specimens such as cells, tissues and macromolecular assemblies. Viruses and related viral assemblies are ideal targets for structural studies that help to define essential biological functions.
openaire   +2 more sources

Single-particle cryo-electron microscopy

Nature Methods, 2015
A brief overview of how to solve a macromolecular structure using single-particle cryo-electron microscopy (cryo-EM).
openaire   +2 more sources

Cryo Electron Microscopy of TRP Channels

2019
Cryo electron microscopy (cryo-EM) is a powerful technique that can be used to elucidate the structural architecture of a protein molecule in a physiologically relevant environment. In this method, purified protein is frozen in its aqueous buffer in a thin layer of vitreous ice in which the biological macromolecules are embedded in various orientations.
Amrita, Samanta, Taylor E T, Hughes, Vera Y, Moiseenkova-Bell   +2 more
openaire   +2 more sources

Cryo-electron Microscopy of Vitreous Sections

2013
More than 30 years ago two groups independently reported the vitrification of pure water, which was until then regarded as impossible without a cryoprotectant [1, 2]. This opened the opportunity to cryo-electron microscopy (cryo-EM) to observe biological samples at nanometer scale, close to their native state. However, poor electron penetration through
Petr, Chlanda, Martin, Sachse
openaire   +2 more sources

Visualisation of the actin cytoskeleton by cryo-electron microscopy

Journal of Cell Science, 2002
An understanding of the mechanisms driving cell motility requires clarification of the structural organisation of actin filament arrays in the regions of cell protrusion termed lamellipodia. Currently, there is a lack of consensus on lamellipodia organisation stemming from the application of alternative procedures for ultrastructural visualisation of ...
Guenter P, Resch, Kenneth N, Goldie, Angelika, Krebs, Andreas, Hoenger, J Victor, Small   +4 more
openaire   +2 more sources

Cryo-Electron Microscopy of DNA

1994
Seeing is believing! Less than 10 years after the discovery of the double helical structure of the DNA, the development by Kleinschmidt and Zahn (Kleinschmidt and Zahn 1959) of a method for direct visualization of nucleic acids has been and remains one of the major achievement of electron microscopy.
J. Dubochet, J. Bednar, P. Furrer, A. Stasiak   +3 more
openaire   +1 more source

Grid Preparation for Cryo-Electron Microscopy

2012
Once 2D crystals suitable for electron crystallography have been obtained, grid preparation for cryo-EM is a critical step in obtaining high-resolution structural information. Specimens have to be prepared in a manner that prevents dehydration and disruption of the crystals in the vacuum of the electron microscope.
openaire   +2 more sources

Cryo-electron microscopy of myelin treated with detergents

Cell and Tissue Research, 1994
After detergent extraction, myelin lamellae disaggregate into diverse globular fragments that correspond to the globular structures observed in normal cryofixed myelin. The interaction of these structural units in the bilayer is probably the morphological basis that provides the stability of the myelin lamellae and their lamination.
openaire   +2 more sources

Cryo-Electron Microscopy and Cryo-Electron Tomography of Viruses

2023
Daniel Luque, José R. Castón
openaire   +1 more source