Results 161 to 170 of about 55,954 (213)

Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies. [PDF]

Acta Crystallogr D Struct Biol
Zafar H, Malone KL, Singh AK, Cianfrocco MA, Glass KC.   +4 more
europepmc   +1 more source

AlignPCA‐2D: PCA‐reduced Euclidean vector alignment for 2D classification in cryo‐EM

Acta Crystallographica Section D, EarlyView.
We introduce AlignPCA‐2D, a PCA‐space Euclidean alignment method for rapid and interpretable 2D classification of cryo‐EM particle images. The approach preserves essential structural variability while greatly reducing computational cost, offering a lightweight alternative to existing large‐scale cryo‐EM classification pipelines.Cryogenic electron ...
E. Ramírez-Aportela, O. L. Zarrabeitia, Y. C. Fonseca, T. Ceska, S. Subramaniam, J. M. Carazo, C. O. S. Sorzano   +6 more
wiley   +1 more source

Uncovering the structural impact of KatG Ser315 mutations in Mycobacterium tuberculosis via cryo-EM. [PDF]

Protein Sci
Allport T, Chaplin AK.
europepmc   +1 more source

Assessing protein‐specific radiation damage in time‐resolved X‐ray solution‐scattering experiments at high‐brilliance synchrotrons using fast detector readout

Acta Crystallographica Section D, EarlyView.
Adenylate kinase was used to quantify how experimental parameters influence radiation damage during time‐resolved X‐ray solution scattering (TR‐XSS) at a multipurpose SAXS beamline. The study provides general guidelines for minimizing radiation artifacts in TR‐XSS.Time‐resolved X‐ray solution scattering (TR‐XSS) enables tracking of protein structural ...
Fatemeh Sabzian-Molaei, Tomás S. Plivelic, Magnus Andersson   +2 more
wiley   +1 more source

A 3.3-Å cryo-EM structure of an engineered high-affinity human prothrombinase complex.

Blood
Üstok FI, Faille A, Huntington JA.
europepmc   +1 more source

Cryo-EM structure of the human COP1-DET1 ubiquitin ligase complex. [PDF]

Nat Commun
Wang S, Teng F, Stjepanovic G, Rao F, Su MY.   +4 more
europepmc   +1 more source

Predicting water at the protein interface in cryo-EM structures from MD-excess chemical potential. [PDF]

Biophys J
Sun Q, Aiyer S, Biswas A, Haldane A, Chatterjee S, Matubayasi N, Lyumkis D, Levy RM.   +7 more
europepmc   +1 more source

Synthesis of a HDAC inhibitor-nanogold probe for cryo-EM visualization in class I HDAC co-repressor complexes. [PDF]

Beilstein J Org Chem
Pytel WA, Schwabe JWR, Hodgkinson JT.
europepmc   +1 more source

Comparing multislice projections of MD simulations with cryo-EM exposes structural prediction errors. [PDF]

Biophys J
Mohammed A, Lincoff J, Natale A, Ophus C, Grabe M, Frost A, Moss FR.   +6 more
europepmc   +1 more source