Results 91 to 100 of about 177,867 (249)
Serial Cryomicrotomy of Saccharomyces cerevisiae for Serial Electron Cryotomography
Bio-Protocol, 2020 Electron cryotomography (cryo-ET) is an increasingly popular technique to study cellular structures and macromolecules in situ. Due to poor penetration of electrons through thick biological samples, the vitreously frozen samples for cryo-ET need to be ...
Cai Tong Ng, Mark Ladinsky, Lu Gan
doaj +1 more source
Nature Communications, 2023 Structures of macromolecules in their native state provide unique unambiguous insights into their functions. Cryo-electron tomography combined with subtomogram averaging demonstrated the power to solve such structures in situ at resolutions in the range ...
Nikita Balyschew +5 more
doaj +1 more source
Waffle Method for optimizing cryo-FIB-milling [PDF]
Microscopy and Microanalysis, 2021 Kotaro Kelley +7 more
openaire +1 more source
Chemistry – A European Journal, EarlyView.An oxo–hydroxyl radical coupling mechanism is identified for O─O bond formation in Photosystem II within the low‐oxidation‐state paradigm. Ca‐bound W3 forms Ox in the S4 state and subsequently couples with O5. The resulting hydroperoxide intermediate provides a mechanistic explanation for the poor resolution of Ox in XFEL structures.
Alireza Ariafard +2 more
wiley +1 more source
Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning
Cytoskeleton, EarlyView.ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham +3 more
wiley +1 more source
Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells
Nature Communications, 2019 The architecture of functional TNTs is still under debate. Here, the authors combine correlative FIB-SEM, light- and cryo-electron microscopy approaches to elucidate the structure of TNTs in neuronal cells, showing that they form structures that are ...
Anna Sartori-Rupp +8 more
doaj +1 more source
Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography
, 2020 Recent advances have made cryogenic (cryo) electron microscopy a key technique to achieve near-atomic-resolution structures of biochemically isolated macromolecular complexes.
Persoon, H. +17 more
core +1 more source
In situ microfluidic cryofixation for cryo Focused Ion Beam milling and cryo electron tomography. [PDF]
, 2019 We present a microfluidic platform for studying structure-function relationships at the cellular level by connecting video rate live cell imaging with in situ microfluidic cryofixation and cryo-electron tomography of near natively preserved, unstained ...
Messling, J. +17 more
core +1 more source
Anatomy of a SPAN Particle: A Four‐Level Structural Framework for Lithium–Sulfur Batteries
EcoEnergy, EarlyView.Sulfurized polyacrylonitrile (SPAN) is a key cathode material for batteries. Borrowing the four‐level classification of protein structure, this perspective demonstrates SPAN's structural complexity into well‐defined levels, giving researchers clear research boundaries and targets at each level for improving battery performance.
Jinkwan Jung +5 more
wiley +1 more source
In situ high-resolution cryo-EM reconstructions from CEMOVIS
IUCrJCryo-electron microscopy can be used to image cells and tissue at high resolution. To ensure electron transparency, the sample thickness must not exceed 500 nm.
Johannes Elferich +6 more
doaj +1 more source