Results 211 to 220 of about 177,867 (249)

Cryo‐FIB‐nanotomography for quantitative analysis of particle structures in cement suspensions

Journal of Microscopy, 2007
SummaryCryo‐FIB‐nanotomography is a novel high‐resolution 3D‐microscopy technique, which opens new possibilities for the quantitative microstructural analysis of complex suspensions. In this paper, we describe the microstructural changes associated with dissolution and precipitation processes occurring in a fresh cement paste, which has high alumina ...
Holzer, L., Gasser, Ph, Kaech, A., Wegmann, M., Zingg, A., Wepf, R., Muench, B.   +6 more
openaire   +7 more sources

Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

Journal of Structural Biology, 2019
Cryo-focused ion beam (FIB)-milling of biological samples can be used to generate thin electron-transparent slices from cells grown or deposited on EM grids.
Shawn Zheng, Georg Wolff, Ronald W Limpens   +2 more
exaly   +2 more sources

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Gentle ions for cryo-FIB milling

Structure, 2023
Cryo-EM imaging of vitreous samples is limited to a few hundred nanometers in thickness. Focused ion beams can mill windows into cells and tissues for imaging, but they damage biological samples. In this issue of Structure, Yang et al. (2023) quantitatively describe this damage and suggest ways to minimize it.
Xiaofeng, Chu, Misha, Kudryashev
openaire   +2 more sources

Cryo-FIB Solution Comparison for Characterization of Indium Microbond Structures

Microscopy and Microanalysis
H. Stegmann, Jaber Derakhshandeh
semanticscholar   +2 more sources

Cryo-FIB developments to enable nanoscale biopsies

Alexander Rigort, Andrew P. Leis
exaly   +2 more sources

Correlative Cryo-FIB Milling using METEOR, an Integrated Fluorescent Light Microscope

Microscopy and Microanalysis, 2022
electron tomography (cryo-ET) is emerging as a powerful technique to acquire high-resolution 3D structures such as intracellular organelles and protein complexes in their near-native cellular environment.
Marit Smeets, Cristina Capitanio, Anna Bieber, Oda H. Schioetz, Philipp S. Erdmann, J. Plitzko   +5 more
semanticscholar   +1 more source

Cryo-FIB-SEM visualization and radiation damage of a water-THF phase-separated mixture and in situ formed organic nanoparticles.

Microscopy
Cryoelectron microscopy is a powerful technique for high-resolution imaging of nonaqueous liquids, but challenges remain regarding imaging and data interpretation.
Satoshi Okada, Hayato Tsuji
semanticscholar   +1 more source

A Next Generation Cryo-FIB Microscope for High-Throughput Cryo-Electron Tomography

Microscopy and Microanalysis, 2022
Dual-beam cryo-FIB/SEM microscopes have become increasingly indispensable tools for cryo-electron tomography over the past decade. Thermo Fisher Scientific has played a leading role in the development together with pioneering scientific groups in the ...
A. Rigort, A. Kotecha, S. Reyntjens, J. Mitchels   +3 more
semanticscholar   +1 more source

Cryo FIB-SEM: Volume imaging of cellular ultrastructure in native frozen specimens [PDF]

Journal of Structural Biology, 2013
Volume microscopy at high resolution is increasingly required to better understand cellular functions in the context of three-dimensional assemblies. Focused ion beam (FIB) milling for serial block face imaging in the scanning electron microscope (SEM ...
Wiebke Möbius, Michael Laue, Torben Ruhwedel   +2 more
exaly   +2 more sources

Topology of the mammalian cell via cryo‐FIB etching

Journal of Microscopy, 2007
SummaryMapping of a mammalian cell down to a feature size of 20–30 nm in 3D is a goal that will answer many questions concerning the connectivity (topology) of a Eukaryotic cell's traffic routes. These routes are defined and separated from one another by the protein‐impregnated lipid membrane barrier of the endoplasmic reticulum (ER).
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