Cryopreservation of common carp sperm [PDF]
, 2003 Experiments were carried out to investigate the effect of five extenders (sucrose, glucose, fructose, KCl and a saline carp sperm extender) and two cryoprotectants (dimethyl-sulfoxide (DMSO) and methanol) on the cryopreservation of common carp sperm ...
Horváth, Ákos +2 more
core +1 more source
Cryopreservation of equine oocytes: looking into the crystal ball [PDF]
, 2020 In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel +4 more
core +2 more sources
Fine morphological assessment of quality of human mature oocytes after slow freezing or vitrification with a closed device: a comparative analysis [PDF]
, 2014 BACKGROUND: Human mature oocytes are very susceptible to cryodamage. Several reports demonstrated that vitrification might preserve oocyte better than slow freezing. However, this is still controversial.
A. Borini +7 more
core +2 more sources
Microfluidics for cryopreservation [PDF]
Biotechnology Advances, 2017 Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell-specific protocols for the addition/removal of cryoprotective agents and freeze-thaw cycles. Microfluidic platforms can revolutionize cryopreservation by providing new tools to manipulate and screen cells at micro/nano
Gang Zhao, Jianping Fu
openaire +3 more sources
Viability of subitaneous eggs of the copepod, Acartia tonsa (Dana), following exposure to various cryoprotectants and hypersaline water [PDF]
, 2009 Subitaneous eggs were obtained from monocultures of the calanoid copepod Acartia tonsa (Dana), Gulf of Mexico strain. Eggs were exposed to methanol, ethylene glycol, propylene glycol, glycerine, and DMSO at 0.0, 0.1, 0.5, 1.0, 2.0, and 5.0 M and ...
Ohsa, Cortney L. +2 more
core +2 more sources
Stepped vitrification technique for human ovarian tissue cryopreservation [PDF]
, 2019 The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation.
Amorin, C.A. +9 more
core +1 more source
Freeze/thaw stress induces organelle remodeling and membrane recycling in cryopreserved human mature oocytes [PDF]
, 2016 Purpose: Our aim was to evaluate the ultrastructure of human metaphase II oocytes subjected to slow freezing and fixed after thawing at different intervals during post-thaw rehydration.
Albani, E. +8 more
core +1 more source
Combinatorial biomaterials discovery strategy to identify new macromolecular cryoprotectants [PDF]
, 2020 Cryoprotective agents (CPAs) are typically solvents or small molecules, but there is a need for innovative CPAs to reduce toxicity and increase cell yield, for the banking and transport of cells.
Gibson, Matthew I. +4 more
core +2 more sources
Vitrification of human immature oocytes before and after in vitro maturation: a review [PDF]
, 2017 The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation
Ashourzadeh, S +5 more
core +1 more source
, 2012 The cryobiology is the science of low temperature biology. Fertility cryopreservation is a vital branch of reproductive science and involves the preservation of gametes (sperm and oocytes), embryos, and reproductive tissues (ovarian and testicular tissues) for use in assisted reproduction techniques (ART).
CIANI, FRANCESCA +3 more
openaire +4 more sources