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Update on techniques for cryopreservation of human spermatozoa
In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable
Chuan Huang +7 more
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Technologies for Vitrification Based Cryopreservation
Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy, tissue ...
Mohammad Amini, James D. Benson
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Antifreeze proteins (AFPs), a class of antifreeze substances, were initially found to bind to ice crystals in vivo, thereby contributing to inhibition of the growth of ice crystals that enabled polar fish to survive at the temperatures below freezing ...
HENG Nuo +8 more
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Cryopreservation of Human Mucosal Leukocytes. [PDF]
BACKGROUND:Understanding how leukocytes in the cervicovaginal and colorectal mucosae respond to pathogens, and how medical interventions affect these responses, is important for developing better tools to prevent HIV and other sexually transmitted ...
Sean M Hughes +19 more
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Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro +4 more
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A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. [PDF]
Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low.
Keitaro Imaizumi +8 more
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Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should
Fatiha Bradaï, Carolina Sánchez-Romero
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The most represented histotype of testicular cancer is the testicular germ-cell tumor (TGCT), both seminoma and non-seminoma. The pathogenesis of this cancer is poorly known.
Andrea Garolla +7 more
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Flow Cytometry of the Side Population: Tips & Tricks
Background: The Side Population (SP) has become an important hallmark for the definition of the stem cell compartment, especially in the detection of these cells and in their physical isolation by fluorescence-activated cell sorting (FACS).
Irene Sales-Pardo +9 more
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Cryopreservation induces changes in the expression of noncoding RNAs in semen: a mini-review [PDF]
Semen cryopreservation is an important approach for preserving male fertility, and thawed semen is often used in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) procedures.
Martin Štimpfel +3 more
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