Results 11 to 20 of about 63,786 (247)

Update on techniques for cryopreservation of human spermatozoa

open access: yesAsian Journal of Andrology, 2022
In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable
Chuan Huang   +7 more
doaj   +1 more source

Technologies for Vitrification Based Cryopreservation

open access: yesBioengineering, 2023
Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy, tissue ...
Mohammad Amini, James D. Benson
doaj   +1 more source

Protective effect and mechanisms of marine antifreeze protein product on sperm cryodamage in livestock

open access: yes浙江大学学报. 农业与生命科学版, 2020
Antifreeze proteins (AFPs), a class of antifreeze substances, were initially found to bind to ice crystals in vivo, thereby contributing to inhibition of the growth of ice crystals that enabled polar fish to survive at the temperatures below freezing ...
HENG Nuo   +8 more
doaj   +1 more source

Cryopreservation of Human Mucosal Leukocytes. [PDF]

open access: yesPLoS ONE, 2016
BACKGROUND:Understanding how leukocytes in the cervicovaginal and colorectal mucosae respond to pathogens, and how medical interventions affect these responses, is important for developing better tools to prevent HIV and other sexually transmitted ...
Sean M Hughes   +19 more
doaj   +1 more source

Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

open access: yesAnimals, 2022
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro   +4 more
doaj   +1 more source

A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. [PDF]

open access: yesPLoS ONE, 2014
Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low.
Keitaro Imaizumi   +8 more
doaj   +1 more source

Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis

open access: yesPlants, 2021
Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should
Fatiha Bradaï, Carolina Sánchez-Romero
doaj   +1 more source

Role of Viral Infections in Testicular Cancer Etiology: Evidence From a Systematic Review and Meta-Analysis

open access: yesFrontiers in Endocrinology, 2019
The most represented histotype of testicular cancer is the testicular germ-cell tumor (TGCT), both seminoma and non-seminoma. The pathogenesis of this cancer is poorly known.
Andrea Garolla   +7 more
doaj   +1 more source

Flow Cytometry of the Side Population: Tips & Tricks

open access: yesCellular Oncology, 2006
Background: The Side Population (SP) has become an important hallmark for the definition of the stem cell compartment, especially in the detection of these cells and in their physical isolation by fluorescence-activated cell sorting (FACS).
Irene Sales-Pardo   +9 more
doaj   +1 more source

Cryopreservation induces changes in the expression of noncoding RNAs in semen: a mini-review [PDF]

open access: yesJournal of Men's Health
Semen cryopreservation is an important approach for preserving male fertility, and thawed semen is often used in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) procedures.
Martin Štimpfel   +3 more
doaj   +1 more source

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