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Placenta, 2008
Over the last few years, there has been renewed interest and scientific debate concerning human oocyte cryopreservation. The aim of this study was to analyse the clinical data coming from our long experience of slow-freezing oocytes. Between 2001 and 2007, 1280 thawing cycles were carried out using oocytes previously frozen by means of a slow 1,2 ...
E, Albani +5 more
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Over the last few years, there has been renewed interest and scientific debate concerning human oocyte cryopreservation. The aim of this study was to analyse the clinical data coming from our long experience of slow-freezing oocytes. Between 2001 and 2007, 1280 thawing cycles were carried out using oocytes previously frozen by means of a slow 1,2 ...
E, Albani +5 more
openaire +2 more sources
Principles of Cryopreservation
2007Cryopreservation is the use of very low temperatures to preserve structurally intact living cells and tissues. Unprotected freezing is normally lethal and this chapter seeks to analyze some of the mechanisms involved and to show how cooling can be used to produce stable conditions that preserve life.
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Experientia, 1988
In this review, advances in cryopreservation of helminth parasites are reported. Our own studies demonstrate that metacestodes of Echinococcus multilocularis can be maintained in a viable state for at least 1-2 years by appropriate deep-freezing and storage in liquid nitrogen.
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In this review, advances in cryopreservation of helminth parasites are reported. Our own studies demonstrate that metacestodes of Echinococcus multilocularis can be maintained in a viable state for at least 1-2 years by appropriate deep-freezing and storage in liquid nitrogen.
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Cryopreservation of Hepatocytes
2014The use of cryopreserved hepatocytes has increased in the last decade due to the improvement of the freezing and thawing methods, and has even achieved acceptance by the US Food and Drug Administration for use in drug metabolizing enzyme induction studies.
Nicola J, Hewitt, Albert P, Li
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Cryopreservation of developing teeth
Cryobiology, 1972Abstract By using a slow rate of cooling, successful storage of developing teeth has been performed in liquid nitrogen. Dye exclusion tests indicate that 78 ± 8% of cells remain viable and transplantation to isologous hosts demonstrates that 86% of the stored developing teeth commence to grow and produce normal dental hard and soft tissue elements ...
Bartlett, PF, Reade, PC
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2014
Oocyte cryopreservation is playing an increasingly important role in the field of human infertility treatment. The ability to store viable oocytes for later use has given many women the option to delay childbearing in order to pursue other ventures in life, without the concern of losing the opportunity to have a family.
Zsolt Peter, Nagy +4 more
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Oocyte cryopreservation is playing an increasingly important role in the field of human infertility treatment. The ability to store viable oocytes for later use has given many women the option to delay childbearing in order to pursue other ventures in life, without the concern of losing the opportunity to have a family.
Zsolt Peter, Nagy +4 more
openaire +2 more sources
Cryopreservation of Hybridomas
2003Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
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Cryopreservation of Rabbit Embryos
Cold Spring Harbor Protocols, 2010INTRODUCTIONIn the rabbit, the technique most frequently used for embryo transfer involves cryopreservation by slow freezing of morulae, followed by surgical implantation of thawed embryos. This method has the advantage of being well controlled and validated: It helps to maintain mitochondrial genomes, preserves the genetic integrity of biological ...
Daniel, Nathalie, Renard, Jean Paul
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Parasitology Today, 1985
Department of Opthalmology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29464, USA Fig. I. The effect of cooling rate on cell survival. Each cell type has its own optimum cooling rate (A). At slower rates the cells are injured by solution effects.
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Department of Opthalmology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29464, USA Fig. I. The effect of cooling rate on cell survival. Each cell type has its own optimum cooling rate (A). At slower rates the cells are injured by solution effects.
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, 2019