Results 311 to 320 of about 163,089 (384)

The Missing Link: Connecting Cultivation Conditions and Refolding Performance via Inclusion Body Biophysical Properties

Biotechnology and Bioengineering, Volume 122, Issue 11, Page 3098-3113, November 2025.
ABSTRACT Inclusion bodies (IBs) frequently form during the expression of heterologous proteins in Escherichia coli and are therefore important in pharmaceutical production. While it is accepted that cultivation conditions affect cultivation performance, the link to refolding performance remains unexplored. This study proposes that this interplay relies,
Matthias Rüdt, Aymerick Bussien, Joan Cortada‐Garcia, Holger Morschett, Hauke Holm, Chiara Mazzucchelli, Karlheinz Flicker   +6 more
wiley   +1 more source

Effects of long-term cryopreservation on sperm quality and oxidative stress in Manchega rams (Ovis aries). [PDF]

Reproduction
Soria-Meneses PJ, Jurado-Campos A, Rubio de Juan A, Montoro Angulo V, Soler AJ, Garde JJ, Ramón Fernández M, Fernández-Santos MDR.   +7 more
europepmc   +1 more source

Endoplasmic Reticulum Stress Amplifies Cytokine Responses in Astrocytes via a PERK/eIF2α/JAK1 Signaling Axis

Glia, Volume 73, Issue 11, Page 2273-2288, November 2025.
Main Points ER stress amplifies TNF‐α, OSM, and IL‐1β responses in astrocytes. PERK and JAK1 signaling are required for the synergistic interaction between ER stress and inflammatory cytokines. Translational suppression attenuates the synthesis of the negative feedback regulators IκBα and SOCS3.
Anirudhya Lahiri, Savannah G. Sims, Jessica A. Herstine, Alicia Meyer, Micah J. Marshall, Ishrat Jahan, Subhodip Adhicary, Allison M. Bradbury, Gordon P. Meares   +8 more
wiley   +1 more source

TdT/Cas12a-based biosensor for sensitive detection of DNA breakpoints in sperm cryopreservation. [PDF]

Sci Rep
Gao B, Liu Z, Yan B, Du K, Pei L, Wang J.   +5 more
europepmc   +1 more source

A randomized comparison of the cryopreservation of human embryos obtained after intracytoplasmic sperm injection with a slow cooling or ultrarapid cooling procedure

, 1999
AL Mauri, CG Petersen, R L R Baruffi, RC Ferreira, JG Franco   +4 more
openalex   +1 more source

Complement dysregulation in human tauopathies

Brain Pathology, Volume 35, Issue 6, November 2025.
Complement protein and activation product levels were assessed in post‐mortem frontal cortex from three distinct subtypes of Tauopathy: Corticobasal degeneration, globular glial tauopathy, and Pick's disease. Quantification of complement proteins by immunohistochemistry and ELISA showed elevation of key complement proteins (C1q) regulators (FI) and ...
Jacqui Nimmo, Samuel Keat, Louis De Muynck, B. Paul Morgan   +3 more
wiley   +1 more source

Semen quality in acute leukemia patients: a retrospective study. [PDF]

Ann Hematol
Buonacquisto A, Del Principe MI, Pallotti F, Bianchini S, Buzzatti E, Caponecchia L, Chiaretti S, Cicolani G, Conflitti AC, Di Chiano S, Fucci L, Kasmi D, Minotti C, Paterno G, Tiravanti I, Venditti A, Lombardo F, Paoli D.   +17 more
europepmc   +1 more source

P-218 Cryopreservation of mature oocytes: The use of a trypsin inhibitor enhances fertilization and obtained embryos rates

, 1997
Andrea Borini, M.G. Bafaro, M.A Bonu, L Serrao, V Distratis, E Sereni   +5 more
openalex   +1 more source

Testicular Function After Immune‐Checkpoint Inhibitors Treatment

Clinical Endocrinology, Volume 103, Issue 5, Page 721-728, November 2025.
ABSTRACT Objective To investigate the effects of immune‐checkpoint inhibitors (ICIs) on spermatogenesis and testicular endocrine function in reproductive‐age men with melanoma. Design Prospective, mixed longitudinal and cross‐sectional cohort study. Patients Twenty‐nine men aged 19–46 years undergoing ICI therapy for melanoma at two Australian centres ...
Andrea Boutros, Amanda Idan, Sue Sleiman, Feyrous Bacha, Ting Zhang, Veena Jayadev, Alexander M. Menzies, Georgina V. Long, David J. Handelsman   +8 more
wiley   +1 more source

Sperm Membrane: Molecular Implications and Strategies for Cryopreservation in Productive Species. [PDF]

Animals (Basel)
Castro M, Leal K, Pezo F, Contreras MJ.
europepmc   +1 more source