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Cryopreservation of Rabbit Embryos
Cold Spring Harbor Protocols, 2010INTRODUCTIONIn the rabbit, the technique most frequently used for embryo transfer involves cryopreservation by slow freezing of morulae, followed by surgical implantation of thawed embryos. This method has the advantage of being well controlled and validated: It helps to maintain mitochondrial genomes, preserves the genetic integrity of biological ...
Daniel, Nathalie, Renard, Jean Paul
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Parasitology Today, 1985
Department of Opthalmology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29464, USA Fig. I. The effect of cooling rate on cell survival. Each cell type has its own optimum cooling rate (A). At slower rates the cells are injured by solution effects.
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Department of Opthalmology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29464, USA Fig. I. The effect of cooling rate on cell survival. Each cell type has its own optimum cooling rate (A). At slower rates the cells are injured by solution effects.
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Experientia, 1988
In this review, advances in cryopreservation of helminth parasites are reported. Our own studies demonstrate that metacestodes of Echinococcus multilocularis can be maintained in a viable state for at least 1-2 years by appropriate deep-freezing and storage in liquid nitrogen.
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In this review, advances in cryopreservation of helminth parasites are reported. Our own studies demonstrate that metacestodes of Echinococcus multilocularis can be maintained in a viable state for at least 1-2 years by appropriate deep-freezing and storage in liquid nitrogen.
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Fertility and Sterility, 2006
To review historical and contemporary advances in oocyte-cryopreservation techniques and outcomes.Publications related to oocyte cryopreservation were identified through MEDLINE and other bibliographic databases.Oocyte cryopreservation can be used as an adjunct to conventional IVF and as an option for fertile women to electively cryopreserve their ...
John K, Jain, Richard J, Paulson
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To review historical and contemporary advances in oocyte-cryopreservation techniques and outcomes.Publications related to oocyte cryopreservation were identified through MEDLINE and other bibliographic databases.Oocyte cryopreservation can be used as an adjunct to conventional IVF and as an option for fertile women to electively cryopreserve their ...
John K, Jain, Richard J, Paulson
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Current Protocols in Immunology, 1997
AbstractLaboratories interested in working with in vitro cultured cells must be able to freeze and thaw cell lines. Such capabilities are necessary to maintain the constancy of the cells since prolonged culture may result in phenotypic and genotypic changes. In addition, cell freezing minimizes problems with contamination as well as the overall expense
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AbstractLaboratories interested in working with in vitro cultured cells must be able to freeze and thaw cell lines. Such capabilities are necessary to maintain the constancy of the cells since prolonged culture may result in phenotypic and genotypic changes. In addition, cell freezing minimizes problems with contamination as well as the overall expense
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Placenta, 2008
Over the last few years, there has been renewed interest and scientific debate concerning human oocyte cryopreservation. The aim of this study was to analyse the clinical data coming from our long experience of slow-freezing oocytes. Between 2001 and 2007, 1280 thawing cycles were carried out using oocytes previously frozen by means of a slow 1,2 ...
E, Albani +5 more
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Over the last few years, there has been renewed interest and scientific debate concerning human oocyte cryopreservation. The aim of this study was to analyse the clinical data coming from our long experience of slow-freezing oocytes. Between 2001 and 2007, 1280 thawing cycles were carried out using oocytes previously frozen by means of a slow 1,2 ...
E, Albani +5 more
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IEEE Engineering in Medicine and Biology Magazine, 1989
The process of cryopreservation is described. Three types of tissue are then considered: allograft heart valves, allograft veins and the musculoskeletal soft tissues of the knee. The problems with each of these tissue types are examined.
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The process of cryopreservation is described. Three types of tissue are then considered: allograft heart valves, allograft veins and the musculoskeletal soft tissues of the knee. The problems with each of these tissue types are examined.
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Seminars in Reproductive Medicine, 1998
Cryopreservation stands as an ongoing evolution in the field of assisted reproductive technologies. Face with increasing numbers of fertilized oocytes and early embryos, cryopreservation avails the ART program of a useful means to preserve embryos for future use without exposing patients to the risks of multiple pregnancies.
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Cryopreservation stands as an ongoing evolution in the field of assisted reproductive technologies. Face with increasing numbers of fertilized oocytes and early embryos, cryopreservation avails the ART program of a useful means to preserve embryos for future use without exposing patients to the risks of multiple pregnancies.
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Cryopreservation of Hybridomas
2003Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
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, 2003