Results 71 to 80 of about 32,701 (270)

Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source

Overcoming intranasal delivery barriers with ultrastable polyzwitterionic siRNA nanocages for enhanced glioblastoma therapy

BMEMat, EarlyView.
A ROS‐responsive, zwitterionic nanocage enables stable, intranasal siRNA delivery to glioblastoma, promoting deep tumor penetration via non‐degradative pathways and trigeminal nerve transport. This platform achieves durable gene silencing and tumor suppression, offering a non‐invasive, storage‐stable strategy for treating glioma and other neurological ...
Jingwen Xie, Jingxing Si, Xiaobei Deng, Jing Tan, Ruijie Zhu, Jizhou Qi, Gaoxia Ma, Bin Wang, Yi Wang, Yunzhou Guo, Yuping Chen, Shiqun Shao, Youqing Shen   +12 more
wiley   +1 more source

Water activity as an indicator of cryoprotectant binding with erythrocytes

, 2010
За допомогою вдосконаленої моделі кріоскопічного осмометра, який дозволяє визначати активність води в біологічних середовищах з точністю ±0,001 Aw, досліджено зміни активності води в суспензіях еритроцитів коня після додавання кріопротекторів 1,2 ...
Зинченко, В.Д., Кучков, В.Н.   +1 more
core   +1 more source

Optimization of the process of cryopreservation of reproductive cells in male pikeperch, Sander lucioperca [PDF]

Caspian Journal of Environmental Sciences
The process for cryopreservation of pikeperch sperm was studied as part of the research. The purpose of these studies is to study the effect of different concentrations of DMSO cryoprotectant during cryopreservation of pikeperch sperm on sperm quality ...
Ainur S. Assylbekova, Gulnaz K. Barinova, Kuanysh N. Syzdykov, Ainura D. Mussina, Gulzhan A. Aubakirova, Aizhan B. Makhanbetova, Zhaxygali B. Kuanchaleyev   +6 more
doaj   +1 more source

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue

Cytometry Part A, EarlyView.
ABSTRACT Archived patient samples (e.g., formalin‐fixed paraffin embedded (FFPE) tissue blocks, cryoembedded tissue blocks, and frozen whole tissue samples) are a source of immune information for which corresponding patient data is often collected.
Justin F. Tirados, Sonya Fenton, Helen M. McGuire, Joanna M. Roberts, Kirsten A. Ward‐Hartstonge, Roslyn A. Kemp   +5 more
wiley   +1 more source

Optimization of the process of cryopreservation of Beluga, Huso huso semen using various cryoprotectants [PDF]

Caspian Journal of Environmental Sciences
Currently, the research on cryopreservation of the reproductive cells plays great importance in the conservation of genetic diversity of many economically and ecologically valuable fish species.
A.S. Assylbekova, K.N. Syzdykov, G.K. Barinova, G.A. Aubakirova, Zh.B. Kuanchaleyev, A.D. Mussina, S.E. Mussin   +6 more
doaj   +1 more source

Strategy to Select an Appropriate Cryoprotectant for an X‑ray Study of Escherichia coli GAPDH Crystals

, 2023
The treatment of protein crystals with an appropriate cryoprotectant and immediate freezing in liquid nitrogen before data collection at the synchrotron source is one of the critical steps for determining the structure of three-dimensional ...
Yong Ju Kim, Hui-Woog Choe, Sandi Hnit San Hlaing, Kui-Jae Lee, Jung Hee Park, Jung Hee Park (8054291), Kiyoung Jang, Nahyun Kim (752460), Hyun Gi Kim, Nahyun Kim, Kiyoung Jang (11760868), Hui-Woog Choe (374831), Hyun Gi Kim (16940356), Sandi Hnit San Hlaing (16940353), Yong Ju Kim (11760874), Kui-Jae Lee (16940359)   +15 more
core   +1 more source

Lycopene‐rich food biomolecules from tomato processing waste: Green recovery, biopolymer‐based stabilisation, functional food applications and health relevance

Food Biomacromolecules, EarlyView.
Abstract Tomato processing waste, particularly peel and pomace, is a sustainable and low‐cost source of lycopene‐rich food biomolecules. Lycopene, a lipophilic carotenoid with an extended conjugated structure, provides intense red colour, antioxidant potential and functional value to tomato‐derived ingredients.
Chandan Kumar Sahu, Mrinmoy Roy
wiley   +1 more source

An Update of Oocyte Vitrification: A Modification of Sucrose and Trehalose as Extracellular Cryoprotectant

, 2018
As well as the development of assisted reproductive technology (ART), as the current treatment of woman who failed in achieving pregnancy, the development of an advance vitrification method also grows rapidly.
Ria Margiana, Silvia W Lestari, Nurin N. Fitriyah   +2 more
core   +1 more source

Independent leaf and root responses to autumn freezing in perennial herbs: Insights from cutting and transplant manipulations

Grassland Research, EarlyView.
In this study, we compared two sampling approaches to assess autumn freezing resistance. Our goal was to determine whether the commonly employed cutting method, which disrupts root‐tissue connectivity, provides a valid measure of true freezing resistance.
Zhengjie Yan, Cheng Chen, Xin Jing, Shuai Ren, Junpeng Rui, Tao Wang, Jin‐Sheng He   +6 more
wiley   +1 more source