Results 81 to 90 of about 32,701 (270)

Room-temperature crystal structure of a metal-dependent W-formate dehydrogenase by serial synchrotron crystallography. [PDF]

open access: yesActa Crystallogr D Struct Biol
We report a room‐temperature serial synchrotron X‐ray crystallographic structural analysis of Nitratidesulfovibrio vulgaris formate dehydrogenase AB, an enzyme that reduces CO2 to formate.Metal‐dependent formate dehydrogenases (Fdhs) are a promising research target in efforts to mitigate climate change by developing active, efficient, selective and ...
Vilela-Alves G   +7 more
europepmc   +2 more sources

Cryoprotection without cryoprotectant [PDF]

open access: yesActa Crystallographica Section A Foundations and Advances, 2017
Yvonne Thielmann   +4 more
openaire   +1 more source

Effect of different methods of cryoprotectant removal on cell recovery.

open access: yes
H9c2 suspension was cooled at 1°C/min to –40°C in the presence of either 5% Me2SO + 6% HES (incubated for 15 minutes at 0°C) or 5% glycerol (incubated for two hours at room temperature).
Elham Ashrafi (8779306)   +2 more
core   +1 more source

Somatic cell reprogramming for Parkinson's disease treatment

open access: yesIbrain, Volume 11, Issue 1, Page 59-73, Spring 2025.
The fundamental purpose of cell reprogramming to treat Parkinson's disease is to generate dopaminergic neurons (DAN) and do transplantation. There are two ways to accomplish this. One method is to induce cells into induced DA neurons (iDAN) directly or to induce cells into induced pluripotent stem cells and ultimately into iDAN in vitro. Another option
Xiaozhuo Li, Kevin Fang, Fengping Wang
wiley   +1 more source

Effect of cryoprotectant on the cryopreservation of [PDF]

open access: yes, 2009
The study evaluated the effect of different cryoprotectants on post-thaw survival and motility of Kolbroek sperm. Semen from Kolbroek boars was collected with the gloved hand technique.
B Sutherland   +11 more
core   +1 more source

Physical stability of RNA–lipid nanoparticles under agitation, freeze–thaw and accelerated stability testing

open access: yesJournal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND The choice of excipient is important in enhancing stability of liquid formulations containing lipid nanoparticles (LNPs) used in RNA delivery where the ability to survive unplanned excursions to higher and lower temperature and vibration is an important attribute.
Franz W Laurett Veras   +2 more
wiley   +1 more source

Effect of the addition of yacon flour (Smallanthus sonchifolius) and freezing on baking performance

open access: yesJournal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND Yacon (Smallanthus sonchifolius), a tuber rich in fructans, presents potential for application in breadmaking due to its nutritional properties and cryoprotectant activity. The aim of this study was to evaluate the incorporation of yacon flour in bread formulations (50 g/kg and 100 g/kg on flour mixture basis) and its impact on ...
Gabriel A. Atanazio‐Silva   +4 more
wiley   +1 more source

Permeation of several cryoprotectant agents into porcine articular cartilage

open access: yes, 2014
Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC)
Law, G.K.   +5 more
core   +1 more source

Coupled ethanol–trehalose processing accelerates thawing of rapid‐frozen cooked rice through water‐state modulation

open access: yesJournal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND Freezing preserves cooked rice, but ice formation and water redistribution can cause structural and textural deterioration. The individual and combined effects of ethanol soaking and trehalose‐assisted cooking on freeze–thaw behaviour, water state, texture, and microstructure were investigated. Rice was soaked in water or an aqueous
Mingyo Ha   +3 more
wiley   +1 more source

A vacuum lyophilization and bacterial tablet-based method for culture medium evaluation and potential use in probiotic products

open access: yesFrontiers in Microbiology
IntroductionThe fundamental step in microbiological detection is the preparation of culture medium. The reliability and accuracy of microbiological assay heavily relies on this step. Currently in China, the most recognized standard method for such medium
Wen Cui   +5 more
doaj   +1 more source

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