Results 121 to 130 of about 7,269 (173)

Cryoprotective agent toxicity interactions in human articular chondrocytes

Cryobiology, 2012
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes.
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.   +6 more
openaire   +3 more sources

The distribution of cryoprotective agents into lipid interfaces

Cryobiology, 1977
Abstract Capillary rise measurements indicate that the cryoprotective agents dimethyl sulfoxido, glycerol, sucrose, hydroxy ethyl starch, polyvinyl pyrrolidone, and dextran are surface active, suggesting the possibility that they distribute into the lipids of the cell membrane.
R J, Williams, D, Harris
openaire   +2 more sources

Perfusion of rabbit kidneys with cryoprotective agents

Cryobiology, 1972
Abstract Rabbit kidneys were perfused at 5 or 37 °C for 2 hr with 2 m solutions of ethylene glycol, glycerol, or dimethylsulfoxide. It was found that each cryoprotectant caused an initial decrease in vascular resistance which was greater at 5 than at 37 °C but that dimethylsulfoxide caused a subsequent increase in resistance which was due to ...
openaire   +2 more sources

Identification of new cryoprotective agents for cultured mammalian cells

In Vitro, 1983
Thirty-one compounds have been identified that act as cryoprotective agents for cultured mammalian cells. Eight compounds were comparable to dimethylsulfoxide (DMSO) in cryoprotective effectiveness. Many of the cryoprotective compounds studied also (a) promote cell fusion and (b) induce cell differentiation in erythroleukemia and other cell systems ...
R J, Klebe, M G, Mancuso
openaire   +2 more sources

The role of cryoprotective agents as hydroxyl radical scavengers

Cryobiology, 1978
Abstract The classic cryoprotective agents dimethylsulfoxide and glycerol are hydroxyl radical scavengers. In addition the cryoprotective agents tetramethylurea, dimethylformide, dimethylurea and monomethylurea act as hydroxyl radical scavengers as shown by the inhibition of ethylene production from methional and the inhibition of methane production ...
J S, Miller, D G, Cornwell
openaire   +2 more sources

Effect of cryoprotective agents on rat cutaneous nerves

Cryobiology, 1975
Abstract Desheathed rat cutaneous nerves were exposed to various concentrations of ethylene glycol (EG), glycerol and dimethyl sulfoxide (DMSO) at temperatures of 1, 24, and 38 °C for periods of time ranging from 5 to 60 min. Measurements of the percent recovery of the original action potential (AP) were determined after removal of the cryoprotective
openaire   +2 more sources

Permeation of several cryoprotectant agents into porcine articular cartilage

Cryobiology, 2009
Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability of osteochondral tissue and enhance clinical outcomes but ...
Rekieh, K., Abazari, A., Elliott, J.A., Jomha, N.M., Law, G.K., McGann, L.E.   +5 more
openaire   +3 more sources

Differing actions of penetrating and nonpenetrating cryoprotective agents

Cryobiology, 1978
Abstract A two-step freezing technique has been used to examine the role of cryoprotective agents during cooling. Chinese hamster fibroblasts were cooled to various subzero holding temperatures and subsequently thawed or cooled to −196 °C before thawing. Cells were suspended in various concentrations of dimethylsulfoxide (DMSO) or hydroxyethyl starch
openaire   +2 more sources

Platelet Preservation by Freezing. Use of Dimethylsulfoxide as Cryoprotective Agent

Transfusion, 1974
Variables important in the preservation of platelets by freezing with dimethylsulfoxide (DMSO) as cryoprotective agent were studied in normal volunteers and thrombocytopenic patients. Use of 5 per cent DMSO and a freezing rate of 1–3 C/min‐ute yielded optimal preservation of platelet viability.
S, Murphy, S N, Sayar, N L, Abdou, F H, Gardner   +3 more
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Distributions During Cryoprotective Agent Loading in a Microchannel

Volume 1B: Extremity; Fluid Mechanics; Gait; Growth, Remodeling, and Repair; Heart Valves; Injury Biomechanics; Mechanotransduction and Sub-Cellular Biophysics; MultiScale Biotransport; Muscle, Tendon and Ligament; Musculoskeletal Devices; Multiscale Mechanics; Thermal Medicine; Ocular Biomechanics; Pediatric Hemodynamics; Pericellular Phenomena; Tissue Mechanics; Biotransport Design and Devices; Spine; Stent Device Hemodynamics; Vascular Solid Mechanics; Student Paper and Design Competitions, 2013
Cryopreservation of cells and tissues is critical to long term storage and off the shelf availability of biomaterials for a variety of disciplines[1]. Typical cryopreservation protocols aim to remove intracellular water by exposing the sample to a cryoprotective agent (CPA) to create an osmotic pressure gradient[2].
Thomas F. Scherr, Shelby Pursley, W. Todd Monroe, Krishnaswamy Nandakumar   +3 more
openaire   +1 more source