Results 111 to 120 of about 7,269 (173)

Structural and Viability Assessment of Bovine Corneas Preserved at an Accessible Low-Temperature (-20 °C) for Eye Irritation Models. [PDF]

ACS Omega
Onorato G, Reis Junior JL, Brandão HM, Munk M.   +3 more
europepmc   +1 more source

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Cryoprotectant agents for ovarian tissue vitrification: Systematic review

Cryobiology, 2021
Studies on the cryopreservation of ovarian tissue usually compare slow freezing versus vitrification and aim to optimize protocols, evaluate combinations or concentrations of cryoprotectant agents (CPAs), exposure time, and the addition of synthetic polymers.
Taynná El Cury-Silva, Monique E.G. Nunes, Maíra Casalechi, Fabio V. Comim, Jhenifer K. Rodrigues, Fernando M. Reis   +5 more
openaire   +2 more sources

Dielectric properties of supercooled cryoprotectant agents

Physics in Medicine and Biology, 1996
The dielectric properties of glycerol, propylene glycol, ethylene glycol and dimethyl sulphoxide have been measured in various aqueous concentrations generally sufficient to allow supercooling rather than freezing. The temperature range investigated is from -80 degrees C to +20 degrees C and the frequency range is 100 MHz to 2 GHz.
S C, Michelson, S, Evans
openaire   +2 more sources

Synthetic Polyampholytes as Macromolecular Cryoprotective Agents

Langmuir, 2018
A series of polyampholytes based on different molar ratios on N, N-dimethylaminopropyl methacrylamide (DMAPMA), acrylic acid (AA), and optionally, N- tert-butylacrylamide ( t-BuAAm), were prepared by free radical copolymerization, and tested as DMSO-free cryoprotective agents for 3T3 fibroblast cells by using a standard freeze-rethaw protocol ...
J. Zhao, M. A. Johnson, R. Fisher, N. A. D. Burke, H. D. H. Stöver   +4 more
openaire   +2 more sources

Cryoprotectant agent toxicity in porcine articular chondrocytes

Cryobiology, 2010
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies have been performed on the toxicity of these agents.
Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.   +5 more
openaire   +3 more sources

Dimethylacetamide, a New Cryoprotective Agent for Platelets

Transfusion, 1971
Dimethylacetamide (DMAC) was used as cryoprotective agent for the preservation of platelets in the frozen state. The addition of dextrose to the platelet suspension greatly increased the cryoprotective effects of this agent. Rat platelets preserved with 5 per cent DMAC and 5 per cent dextrose showed a higher percentage of morphologically intact cells ...
I, Djerassi, A, Roy, J, Kim, J, Cavins
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Thermal Expansion Measurements of Cryoprotective Agents

Advances in Bioengineering, 2003
As part of an ongoing effort to characterize the mechanical behavior of biological tissues in the cryogenic temperature range, the current study focuses on the thermal expansion measurements of cryoprotective agents. This study focuses particularly on the upper part of the cryogenic temperature range, where the cryoprotectant behaves like low viscous ...
Yoed Rabin, Ernest Bell
openaire   +1 more source

Deep eutectic solvents as cryoprotective agents for mammalian cells

Journal of Materials Chemistry B, 2022
A deep eutectic solvent is an effective cryoprotective agent for several human cell lines.
Saffron J. Bryant, Miyah N. Awad, Aaron Elbourne, Andrew J. Christofferson, Andrew V. Martin, Nastaran Meftahi, Calum J. Drummond, Tamar L. Greaves, Gary Bryant   +8 more
openaire   +2 more sources

A Dilution-Filtration System for Removing Cryoprotective Agents

Journal of Biomechanical Engineering, 2011
In most cryopreservation applications, the final concentrations of cryoprotective agents (CPAs) must be reduced to biocompatible levels. However, traditional methods for removing CPAs usually have disadvantages of operation complexity, time consumption, and ease of contamination, especially for the applications involving large volumes of cell ...
Xiaoming, Zhou, Zhong, Liu, Zhiquan, Shu, Weiping, Ding, Pingan, Du, JaeHyun, Chung, Carolyn, Liu, Shelly, Heimfeld, Dayong, Gao   +8 more
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Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

2020
Quantification of the amount of cryoprotective agent (CPA) in a tissue is an essential step in the design of successful cryopreservation protocols. This chapter details two inexpensive methods to measure cryoprotective agent permeation into tissues as functions of time.
Kezhou, Wu, Leila, Laouar, Nadia, Shardt, Janet A W, Elliott, Nadr M, Jomha   +4 more
openaire   +2 more sources