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Preservation of Malassezia pachydermatis using skimmed milk as cryoprotective agent
Journal of Microbiological Methods, 2017This study evaluated the effect of skimmed milk as a cryoprotectant for the maintenance and long-term preservation of 70 Malassezia pachydermatis strains. An initial suspension of each strain was prepared in sterile distilled water with skimmed milk. The most effective method was cryopreservation that ensured 100% viability for 2years.
F.A. Silva Sergent +7 more
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Cryo letters, 2019
Understanding the spatiotemporal distribution characteristics of cryoprotectant concentration in the cartilage tissue during loading is essential for the rational design of the loading procedure and the successful cryopreservation of articular cartilage (AC).To construct a diffusion mass transfer model to describe the permeation of cryoprotectant into ...
X, Yu, G, Chen
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Understanding the spatiotemporal distribution characteristics of cryoprotectant concentration in the cartilage tissue during loading is essential for the rational design of the loading procedure and the successful cryopreservation of articular cartilage (AC).To construct a diffusion mass transfer model to describe the permeation of cryoprotectant into ...
X, Yu, G, Chen
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[Comparison of platelets frozen with different cryoprotective agents].
Comptes rendus des seances de la Societe de biologie et de ses filiales, 1982A comparative study was performed on platelets frozen with dimethyl-sulfoxide (D.M.S.O.), glycérol and dimethyl-acetamide (D.M.A.C.). The results of tests realized in vitro (platelet yields after thawing platelet aggregation, hypotonic stress, release of enzymes and ultrastructure), and in vivo (life span and recoveries after transfusion) have clearly ...
G, Vezon +8 more
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Cryoprotectant agent characterization via acoustical and optical analyses
The Journal of the Acoustical Society of AmericaIn the field of cryopreservation, there exists a class of substances known as cryoprotectant agents (CPAs), which display an ability to vitrify. These CPAs are used to prevent cellular damage during the preservation of human tissues. In a vitrified state, cells exist in a glass-like state, meaning there is no formation of mechanically damaging ice ...
Alicia Casacchia +4 more
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Mechanisms of cryoprotection of cells by intracellular cryoprotective agents
Cryobiology, 1989openaire +1 more source
The surface active properties of cryoprotective agents
Cryobiology, 1975R.J. Williams, D. Harris
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