Results 151 to 160 of about 3,957 (196)

Identification of new cryoprotective agents for cultured mammalian cells

In Vitro, 1983
Thirty-one compounds have been identified that act as cryoprotective agents for cultured mammalian cells. Eight compounds were comparable to dimethylsulfoxide (DMSO) in cryoprotective effectiveness. Many of the cryoprotective compounds studied also (a) promote cell fusion and (b) induce cell differentiation in erythroleukemia and other cell systems ...
Robert J Klebe
exaly   +3 more sources

Cryoprotectant agent toxicity in porcine articular chondrocytes

Cryobiology, 2010
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies have been performed on the toxicity of these agents.
Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.   +5 more
openaire   +3 more sources

Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

2020
Quantification of the amount of cryoprotective agent (CPA) in a tissue is an essential step in the design of successful cryopreservation protocols. This chapter details two inexpensive methods to measure cryoprotective agent permeation into tissues as functions of time.
Kezhou, Wu, Leila, Laouar, Nadia, Shardt, Janet A W, Elliott, Nadr M, Jomha   +4 more
openaire   +2 more sources

Perfusion of rabbit kidneys with cryoprotective agents

Cryobiology, 1972
Abstract Rabbit kidneys were perfused at 5 or 37 °C for 2 hr with 2 m solutions of ethylene glycol, glycerol, or dimethylsulfoxide. It was found that each cryoprotectant caused an initial decrease in vascular resistance which was greater at 5 than at 37 °C but that dimethylsulfoxide caused a subsequent increase in resistance which was due to ...
openaire   +2 more sources

Cryoprotectant agents for ovarian tissue vitrification: Systematic review

Cryobiology, 2021
Studies on the cryopreservation of ovarian tissue usually compare slow freezing versus vitrification and aim to optimize protocols, evaluate combinations or concentrations of cryoprotectant agents (CPAs), exposure time, and the addition of synthetic polymers.
Taynná El Cury-Silva, Monique E.G. Nunes, Maíra Casalechi, Fabio V. Comim, Jhenifer K. Rodrigues, Fernando M. Reis   +5 more
openaire   +2 more sources

Thermal Expansion Measurements of Cryoprotective Agents

Advances in Bioengineering, 2003
As part of an ongoing effort to characterize the mechanical behavior of biological tissues in the cryogenic temperature range, the current study focuses on the thermal expansion measurements of cryoprotective agents. This study focuses particularly on the upper part of the cryogenic temperature range, where the cryoprotectant behaves like low viscous ...
Yoed Rabin, Ernest Bell
openaire   +1 more source

The mechanism of action of low temperature and cryoprotective agents on immunoproteins

Cryobiology, 1978
Abstract The freezing and thawing of specific antitoxic serum proteins without a cryoprotective agent results in a reduction of titer. A freezing regimen has been chosen and the cryoprotectant PEO-400 has been added to prevent a reduction in activity of antitetanus serum after 6 months of storage in liquid nitrogen. The study of dielectric properties
A A, Tsutsayeva, N S, Pushkar, V M, Markova, Y A, Itkin, A L, Markovsky, I I, Gavrilova, L V, Ivanov, V A, Moiseyev, G G, Medvedyeva   +8 more
openaire   +2 more sources

Effect of cryoprotective agents on rat cutaneous nerves

Cryobiology, 1975
Abstract Desheathed rat cutaneous nerves were exposed to various concentrations of ethylene glycol (EG), glycerol and dimethyl sulfoxide (DMSO) at temperatures of 1, 24, and 38 °C for periods of time ranging from 5 to 60 min. Measurements of the percent recovery of the original action potential (AP) were determined after removal of the cryoprotective
openaire   +2 more sources

Differing actions of penetrating and nonpenetrating cryoprotective agents

Cryobiology, 1978
Abstract A two-step freezing technique has been used to examine the role of cryoprotective agents during cooling. Chinese hamster fibroblasts were cooled to various subzero holding temperatures and subsequently thawed or cooled to −196 °C before thawing. Cells were suspended in various concentrations of dimethylsulfoxide (DMSO) or hydroxyethyl starch
openaire   +2 more sources

Evaluation of ethylene glycol as a cryoprotective agent for blood platelets

Cryobiology, 1973
Abstract (1) Ethylene glycol appears to penetrate blood platelets rapidly and is nontoxic in high concentrations. (2) Higher concentrations are tolerated when potassium is present in the suspending medium. (3) Although ethylene glycol satisfied the basic theoretical criteria for a penetrating cryoprotectant, it failed to result in sufficiently high ...
R A, Kahn, L J, Flinton
openaire   +2 more sources