Results 191 to 200 of about 7,710 (246)
Some of the next articles are maybe not open access.
A Dilution-Filtration System for Removing Cryoprotective Agents
Journal of Biomechanical Engineering, 2011In most cryopreservation applications, the final concentrations of cryoprotective agents (CPAs) must be reduced to biocompatible levels. However, traditional methods for removing CPAs usually have disadvantages of operation complexity, time consumption, and ease of contamination, especially for the applications involving large volumes of cell ...
Xiaoming, Zhou +8 more
openaire +2 more sources
Osmometric Measurements of Cryoprotective Agent Permeation into Tissues
2020Quantification of the amount of cryoprotective agent (CPA) in a tissue is an essential step in the design of successful cryopreservation protocols. This chapter details two inexpensive methods to measure cryoprotective agent permeation into tissues as functions of time.
Kezhou, Wu +4 more
openaire +2 more sources
Cryoprotective agent toxicity interactions in human articular chondrocytes
Cryobiology, 2012Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes.
Law, G. K. +6 more
openaire +3 more sources
The distribution of cryoprotective agents into lipid interfaces
Cryobiology, 1977Abstract Capillary rise measurements indicate that the cryoprotective agents dimethyl sulfoxido, glycerol, sucrose, hydroxy ethyl starch, polyvinyl pyrrolidone, and dextran are surface active, suggesting the possibility that they distribute into the lipids of the cell membrane.
R J, Williams, D, Harris
openaire +2 more sources
Perfusion of rabbit kidneys with cryoprotective agents
Cryobiology, 1972Abstract Rabbit kidneys were perfused at 5 or 37 °C for 2 hr with 2 m solutions of ethylene glycol, glycerol, or dimethylsulfoxide. It was found that each cryoprotectant caused an initial decrease in vascular resistance which was greater at 5 than at 37 °C but that dimethylsulfoxide caused a subsequent increase in resistance which was due to ...
openaire +2 more sources
Identification of new cryoprotective agents for cultured mammalian cells
In Vitro, 1983Thirty-one compounds have been identified that act as cryoprotective agents for cultured mammalian cells. Eight compounds were comparable to dimethylsulfoxide (DMSO) in cryoprotective effectiveness. Many of the cryoprotective compounds studied also (a) promote cell fusion and (b) induce cell differentiation in erythroleukemia and other cell systems ...
R J, Klebe, M G, Mancuso
openaire +2 more sources
The role of cryoprotective agents as hydroxyl radical scavengers
Cryobiology, 1978Abstract The classic cryoprotective agents dimethylsulfoxide and glycerol are hydroxyl radical scavengers. In addition the cryoprotective agents tetramethylurea, dimethylformide, dimethylurea and monomethylurea act as hydroxyl radical scavengers as shown by the inhibition of ethylene production from methional and the inhibition of methane production ...
J S, Miller, D G, Cornwell
openaire +2 more sources
Effect of cryoprotective agents on rat cutaneous nerves
Cryobiology, 1975Abstract Desheathed rat cutaneous nerves were exposed to various concentrations of ethylene glycol (EG), glycerol and dimethyl sulfoxide (DMSO) at temperatures of 1, 24, and 38 °C for periods of time ranging from 5 to 60 min. Measurements of the percent recovery of the original action potential (AP) were determined after removal of the cryoprotective
openaire +2 more sources
Permeation of several cryoprotectant agents into porcine articular cartilage
Cryobiology, 2009Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability of osteochondral tissue and enhance clinical outcomes but ...
Rekieh, K. +5 more
openaire +3 more sources
Differing actions of penetrating and nonpenetrating cryoprotective agents
Cryobiology, 1978Abstract A two-step freezing technique has been used to examine the role of cryoprotective agents during cooling. Chinese hamster fibroblasts were cooled to various subzero holding temperatures and subsequently thawed or cooled to −196 °C before thawing. Cells were suspended in various concentrations of dimethylsulfoxide (DMSO) or hydroxyethyl starch
openaire +2 more sources