Results 191 to 200 of about 7,623 (251)

Synthetic Polyampholytes as Macromolecular Cryoprotective Agents

Langmuir, 2018
A series of polyampholytes based on different molar ratios on N, N-dimethylaminopropyl methacrylamide (DMAPMA), acrylic acid (AA), and optionally, N- tert-butylacrylamide ( t-BuAAm), were prepared by free radical copolymerization, and tested as DMSO-free cryoprotective agents for 3T3 fibroblast cells by using a standard freeze-rethaw protocol ...
J. Zhao, M. A. Johnson, R. Fisher, N. A. D. Burke, H. D. H. Stöver   +4 more
openaire   +2 more sources

Dimethylacetamide, a New Cryoprotective Agent for Platelets

Transfusion, 1971
Dimethylacetamide (DMAC) was used as cryoprotective agent for the preservation of platelets in the frozen state. The addition of dextrose to the platelet suspension greatly increased the cryoprotective effects of this agent. Rat platelets preserved with 5 per cent DMAC and 5 per cent dextrose showed a higher percentage of morphologically intact cells ...
I, Djerassi, A, Roy, J, Kim, J, Cavins
openaire   +2 more sources

The distribution of cryoprotective agents into lipid interfaces

Cryobiology, 1977
Abstract Capillary rise measurements indicate that the cryoprotective agents dimethyl sulfoxido, glycerol, sucrose, hydroxy ethyl starch, polyvinyl pyrrolidone, and dextran are surface active, suggesting the possibility that they distribute into the lipids of the cell membrane.
R J, Williams, D, Harris
openaire   +2 more sources

A Dilution-Filtration System for Removing Cryoprotective Agents

Journal of Biomechanical Engineering, 2011
In most cryopreservation applications, the final concentrations of cryoprotective agents (CPAs) must be reduced to biocompatible levels. However, traditional methods for removing CPAs usually have disadvantages of operation complexity, time consumption, and ease of contamination, especially for the applications involving large volumes of cell ...
Xiaoming, Zhou, Zhong, Liu, Zhiquan, Shu, Weiping, Ding, Pingan, Du, JaeHyun, Chung, Carolyn, Liu, Shelly, Heimfeld, Dayong, Gao   +8 more
openaire   +2 more sources

Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

2020
Quantification of the amount of cryoprotective agent (CPA) in a tissue is an essential step in the design of successful cryopreservation protocols. This chapter details two inexpensive methods to measure cryoprotective agent permeation into tissues as functions of time.
Kezhou, Wu, Leila, Laouar, Nadia, Shardt, Janet A W, Elliott, Nadr M, Jomha   +4 more
openaire   +2 more sources

The role of cryoprotective agents as hydroxyl radical scavengers

Cryobiology, 1978
Abstract The classic cryoprotective agents dimethylsulfoxide and glycerol are hydroxyl radical scavengers. In addition the cryoprotective agents tetramethylurea, dimethylformide, dimethylurea and monomethylurea act as hydroxyl radical scavengers as shown by the inhibition of ethylene production from methional and the inhibition of methane production ...
J S, Miller, D G, Cornwell
openaire   +2 more sources

Cryoprotectant agent toxicity in porcine articular chondrocytes

Cryobiology, 2010
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies have been performed on the toxicity of these agents.
Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.   +5 more
openaire   +3 more sources

Cryoprotectant agents for ovarian tissue vitrification: Systematic review

Cryobiology, 2021
Studies on the cryopreservation of ovarian tissue usually compare slow freezing versus vitrification and aim to optimize protocols, evaluate combinations or concentrations of cryoprotectant agents (CPAs), exposure time, and the addition of synthetic polymers.
Taynná El Cury-Silva, Monique E.G. Nunes, Maíra Casalechi, Fabio V. Comim, Jhenifer K. Rodrigues, Fernando M. Reis   +5 more
openaire   +2 more sources

Perfusion of rabbit kidneys with cryoprotective agents

Cryobiology, 1972
Abstract Rabbit kidneys were perfused at 5 or 37 °C for 2 hr with 2 m solutions of ethylene glycol, glycerol, or dimethylsulfoxide. It was found that each cryoprotectant caused an initial decrease in vascular resistance which was greater at 5 than at 37 °C but that dimethylsulfoxide caused a subsequent increase in resistance which was due to ...
openaire   +2 more sources

Deep eutectic solvents as cryoprotective agents for mammalian cells

Journal of Materials Chemistry B, 2022
A deep eutectic solvent is an effective cryoprotective agent for several human cell lines.
Saffron J. Bryant, Miyah N. Awad, Aaron Elbourne, Andrew J. Christofferson, Andrew V. Martin, Nastaran Meftahi, Calum J. Drummond, Tamar L. Greaves, Gary Bryant   +8 more
openaire   +2 more sources