Results 201 to 210 of about 7,623 (251)

Effect of cryoprotective agents on rat cutaneous nerves

Cryobiology, 1975
Abstract Desheathed rat cutaneous nerves were exposed to various concentrations of ethylene glycol (EG), glycerol and dimethyl sulfoxide (DMSO) at temperatures of 1, 24, and 38 °C for periods of time ranging from 5 to 60 min. Measurements of the percent recovery of the original action potential (AP) were determined after removal of the cryoprotective
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Thermal Expansion Measurements of Cryoprotective Agents

Advances in Bioengineering, 2003
As part of an ongoing effort to characterize the mechanical behavior of biological tissues in the cryogenic temperature range, the current study focuses on the thermal expansion measurements of cryoprotective agents. This study focuses particularly on the upper part of the cryogenic temperature range, where the cryoprotectant behaves like low viscous ...
Yoed Rabin, Ernest Bell
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Platelet Preservation by Freezing. Use of Dimethylsulfoxide as Cryoprotective Agent

Transfusion, 1974
Variables important in the preservation of platelets by freezing with dimethylsulfoxide (DMSO) as cryoprotective agent were studied in normal volunteers and thrombocytopenic patients. Use of 5 per cent DMSO and a freezing rate of 1–3 C/min‐ute yielded optimal preservation of platelet viability.
S, Murphy, S N, Sayar, N L, Abdou, F H, Gardner   +3 more
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Identification of new cryoprotective agents for cultured mammalian cells

In Vitro, 1983
Thirty-one compounds have been identified that act as cryoprotective agents for cultured mammalian cells. Eight compounds were comparable to dimethylsulfoxide (DMSO) in cryoprotective effectiveness. Many of the cryoprotective compounds studied also (a) promote cell fusion and (b) induce cell differentiation in erythroleukemia and other cell systems ...
R J, Klebe, M G, Mancuso
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Differing actions of penetrating and nonpenetrating cryoprotective agents

Cryobiology, 1978
Abstract A two-step freezing technique has been used to examine the role of cryoprotective agents during cooling. Chinese hamster fibroblasts were cooled to various subzero holding temperatures and subsequently thawed or cooled to −196 °C before thawing. Cells were suspended in various concentrations of dimethylsulfoxide (DMSO) or hydroxyethyl starch
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Evaluation of ethylene glycol as a cryoprotective agent for blood platelets

Cryobiology, 1973
Abstract (1) Ethylene glycol appears to penetrate blood platelets rapidly and is nontoxic in high concentrations. (2) Higher concentrations are tolerated when potassium is present in the suspending medium. (3) Although ethylene glycol satisfied the basic theoretical criteria for a penetrating cryoprotectant, it failed to result in sufficiently high ...
R A, Kahn, L J, Flinton
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The mechanism of action of low temperature and cryoprotective agents on immunoproteins

Cryobiology, 1978
Abstract The freezing and thawing of specific antitoxic serum proteins without a cryoprotective agent results in a reduction of titer. A freezing regimen has been chosen and the cryoprotectant PEO-400 has been added to prevent a reduction in activity of antitetanus serum after 6 months of storage in liquid nitrogen. The study of dielectric properties
A A, Tsutsayeva, N S, Pushkar, V M, Markova, Y A, Itkin, A L, Markovsky, I I, Gavrilova, L V, Ivanov, V A, Moiseyev, G G, Medvedyeva   +8 more
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Effects of cryoprotective agents and freezing on abdominal ganglia of Aplysia

Cryobiology, 1979
Isolated Aplysia depilans abdominal ganglia were exposed to 10 and 20% dimethylsulphoxide (Me2SO) or glycerol at room temperature. Results indicate that Me2SO induced an irreversible depression of extracellularly recorded ganglionic spontaneous spike generation while glycerol proved to be non-toxic.
M, Pasic, L, De Sa Faria
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Cryoprotective agent toxicity interactions in human articular chondrocytes

Cryobiology, 2012
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes.
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.   +6 more
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A Network Thermodynamic Model of Kidney Perfusion With a Cryoprotective Agent

Journal of Biomechanical Engineering, 1999
A network thermodynamic model has been devised to describe the coupled movement of water and a permeable additive within a kidney during perfusion under the combined action of diffusive, hydrodynamic, and mechanical processes. The model has been validated by simulating perfusions with Me2SO, glycerol, and sucrose and comparing predicted weight and ...
C A, Lachenbruch, K R, Diller
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