Results 61 to 70 of about 3,438 (209)

Research Progresses in Vitrification by Minimum Volume Method

open access: yesZhileng xuebao, 2018
Vitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents.
Zhang Xiaomin, Zhou Xinli
doaj  

Microfluidic Determination of Distinct Membrane Transport Properties between Lung Adenocarcinoma Cells CL1-0 and CL1-5

open access: yesBiosensors, 2022
The cell membrane permeability of a cell type to water (Lp) and cryoprotective agents (Ps), is the key factor that determines the optimal cooling and mass transportation during cryopreservation.
Chiu-Jen Chen   +4 more
doaj   +1 more source

Ascorbic acid 2‐glucoside improves survival, quality, and fertility of frozen‐thawed C57Bl/6J and C57Bl/6N mouse spermatozoa

open access: yesAndrology, Volume 13, Issue 6, Page 1601-1614, September 2025.
Abstract Background Ascorbic acid 2‐glucoside (AA2G) is a stabilized form of ascorbic acid and a potent antioxidant. Ascorbic acid is present in the testes and epididymis and helps maintain the physiological integrity of reproductive organs. Its properties have been utilized to protect spermatozoa of different species from oxidative stress.
Marcello Raspa   +2 more
wiley   +1 more source

Cryo-derived plants through embryogenesis showed same levels of vinblastine and vincristine (anticancer) in Catharanthus roseus and had normal genome size

open access: yesScientific Reports, 2022
Cryopreservation of rare plant materials is an important approach for preserving germplasms and is a good added concept to tissue banking. The preservation of embryogenic cell suspensions is even more valuable as the tissues facilitate in producing ...
A. Mujib   +2 more
doaj   +1 more source

Comprehensive Tools for Culturing Blastocystis: A Standardized Resource for Research and Diagnostics

open access: yesCurrent Protocols, Volume 5, Issue 8, August 2025.
Abstract Blastocystis spp. is a widely prevalent anaerobic protozoan of uncertain pathogenicity found in the gastrointestinal tracts of over 1 billion people worldwide. Despite its potential significance in health and disease, Blastocystis spp. remains challenging to culture axenically due to its anaerobic nature and the diversity of its genetic ...
Daisy Shaw   +8 more
wiley   +1 more source

Cryopreservation of farm animal gametes and embryos: recent updates and progress [PDF]

open access: yesFrontiers of Agricultural Science and Engineering, 2019
Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation
Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU
doaj   +1 more source

Cryomicroneedle Arrays for Biotherapeutics Delivery

open access: yesSmall Science, Volume 5, Issue 8, August 2025.
CryoMNs for biotherapeutic delivery to treat various diseases. CryoMNs: cryomicroneedles. Biotherapy offers a promising approach for treating a variety of diseases. However, the lack of advanced delivery systems remains a significant barrier to improve the efficacy, safety, and cost‐effectiveness of biotherapeutics.
Chunli Yang   +9 more
wiley   +1 more source

Use of Human In Vitro Gut Specimens for Translational Neurogastroenterology and Motility in the 21st Century

open access: yesNeurogastroenterology &Motility, Volume 37, Issue 8, August 2025.
In vitro studies with GI surgical specimens or biopsy are a critical step in translating preclinical studies in animals to humans before testing potential therapeutic interventions in expensive clinical trials. Routine availability of human gut specimens, in combination with technological advances and powerful new in vitro models of the human ENS from ...
Dmitrii Pavlov, Fievos L. Christofi
wiley   +1 more source

In Vivo Cytosolic Delivery of Biomolecules into Neurons for Super‐Resolution Imaging and Genome Modification

open access: yesAdvanced Science, Volume 12, Issue 25, July 3, 2025.
The N1 peptide specifically targets neurons, enabling cytosolic delivery of fluorescent dyes and proteins for super‐resolution imaging and functional genetic modification. Abstract Efficient delivery of biomolecules into neurons has significant impacts on therapeutic applications in the central nervous system (CNS) and fundamental neuroscience research.
Xiaoqian Ge   +16 more
wiley   +1 more source

Analysis of Thermal and Crystallization in Cryoprotective Agents

open access: yesZhileng xuebao, 2014
Ice crystal formation is one of the factors that causes cell damage during crvopreservation process.Ice crystal will provide fundamental information for the development of appropriate cryopreservation protocols.Effects of concentration and properties of ...
Yang Bo   +3 more
doaj  

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