Results 61 to 70 of about 45,860 (139)

Cryopreservation of Endothelial Cells in Various Cryoprotective Agents and Media - Vitrification versus Slow Freezing Methods. [PDF]

PLoS ONE, 2016
Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled ...
Achim von Bomhard, Alexander Elsässer, Lucas Maximilian Ritschl, Silke Schwarz, Nicole Rotter   +4 more
doaj   +1 more source

Evaluation of Ethylene Glycol as a Cryoprotectant in the Sperm Cryopreservation of Trans-andean Shovelnose Catfish (Sorubim Cuspicaudus, Pimelodidae)

Acta Biológica Colombiana, 2014
The catfish Sorubim cuspicaudus cryopreservation semen was evaluated using three levels (5, 10, 15%) of ethylene glycol (ETG). Males (n = 13) undergoing spermiation and in final maturation females (n = 6) were induced with 0.4 ml Ovaprim®/Kg, after 12 ...
Victor Julio Atencio García, Maria Dorado, Emilio Navarro, Francisco Pérez, Briner Herrera, Jorge Movilla, José Espinosa-Araujo   +6 more
doaj   +1 more source

Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion

Small, Volume 21, Issue 11, March 19, 2025.
In this study, a vascular microphysiological system is designed to simulate physiologically relevant variable wall shear stress on a chip, while incorporating electrical impedance measurement for real‐time quantification of endothelial barrier function.
Yongdeok Kim, Ishan Goswami, Elisabeth Gill, S. Reza Mahmoodi, Anthony N. Consiglio, Jazmin Velazquez, Gabriel Nieman, Alexis Abigail A. Alburo, Brady Woods, Bradley W. Ellis, Irina Filz von Reiterdank, Korkut Uygun, Basak E. Uygun, Boris Rubinsky, Kevin E. Healy   +14 more
wiley   +1 more source

Genomic and functional evaluation of exopolysaccharide produced by Liquorilactobacillus mali t6-52: technological implications

Microbial Cell Factories
Background This study explores the biosynthesis, characteristics, and functional properties of exopolysaccharide produced by the strain Liquorilactobacillus mali T6-52. The strain demonstrated significant EPS production with a non-ropy phenotype. Results
Manyu Wu, Shadi Pakroo, Chiara Nadai, Zeno Molinelli, Immacolata Speciale, Crisitina De Castro, Armin Tarrah, Jijin Yang, Alessio Giacomini, Viviana Corich   +9 more
doaj   +1 more source

Cooperative Heterogeneous Deep Reinforcement Learning [PDF]

arXiv, 2020
Numerous deep reinforcement learning agents have been proposed, and each of them has its strengths and flaws. In this work, we present a Cooperative Heterogeneous Deep Reinforcement Learning (CHDRL) framework that can learn a policy by integrating the advantages of heterogeneous agents.
arxiv  

Successful Cryoprotectant‐Free Vitrification of Honey Bee (Apis mellifera) Drone Sperm With Royal Jelly Supplemented Extender

Veterinary Medicine and Science, Volume 11, Issue 2, March 2025.
Our results showed that adding a 1% royal jelly supplement to the cryoprotectant‐free vitrification media in the droplet method improves drone sperm quality parameters post‐warming. Our findings provide valuable insights into optimizing drone sperm preservation, contributing to the conservation of these vital pollinators.
Aliakbar Mohammadi, Adel Sabrivand, Hadi Hajarian   +2 more
wiley   +1 more source

Analysis of Thermal and Crystallization in Cryoprotective Agents

Zhileng xuebao, 2014
Ice crystal formation is one of the factors that causes cell damage during crvopreservation process．Ice crystal will provide fundamental information for the development of appropriate cryopreservation protocols．Effects of concentration and properties of ...
Yang Bo, Liu Baolin, Shen Li, Zhang Sishi   +3 more
doaj  

Fertilizing capacity of cryopreserved sperm of Chirostoma jordani (Woolman, 1894)

Fisheries and Aquatic Sciences
The genus Chirostoma is endemic from the Mesa Central of Mexico. It is conformed of 18 species and six subspecies. Five species are in some category of risk, because of this, Chirostoma jordani is an excellent model species to implement biotechnologies ...
Bustamante-González Jesús Dámaso, Gutiérrez-Díaz Dulce Leticia, Baca-Alejo Judith Sarai, Figueroa-Lucero Gerardo, Arenas-Ríos Edith, Hernández-Rubio María Cecilia, Ávalos-Rodríguez Alejandro   +6 more
doaj   +1 more source

Reconsideration of the safety and effectiveness of human oocyte cryopreservation

Reproductive Biology and Endocrinology, 2023
Mature oocyte cryopreservation (OC) has become increasingly common since the American Society for Reproductive Medicine declared OC to no longer be experimental.
Helen C. K. Kwan
doaj   +1 more source

Protective Effect of B1 and B12 Vitamins on Post‐Thaw Sperm Quality Parameters and Seminal Plasma Antioxidant Status in Rams

Veterinary Medicine and Science, Volume 11, Issue 2, March 2025.
This study investigated the in vitro effects of B1 and B12 vitamins on sperm quality and seminal antioxidant activity in rams. The results showed that adding 1 mg/mL VitB12 or 0.5 mg/mL VitB1 to the diluent significantly improved ram sperm quality and antioxidant function in semen.
Saleh Tabatabaei Vakili, Roghayeh Zeidi, Robab Nabhani   +2 more
wiley   +1 more source