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Biosynthesis of Sterols and Triterpenoids in Tissue Cultures of Cucurbita maxima

Planta Medica, 1983
The biosynthesis of sterols and triterpenoids in CUCURBITA MAXIMA was studied by analysis of unsaponifiable fraction of tissues from different development stages of the plant (seeds, seedlings, adult plant and tissue culture) and by feeding germinating seeds and tissue cultures with [2- (14)C]-acetate.
O, Caputo   +4 more
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Synthesis of P-protein in mature phloem of Cucurbita maxima

Planta, 1976
Cotyledons of Cucurbita maxima Duch. seedlings were provided with (14)C-labeled amino acids for 12 h. Besides the bulk of labeled amino acids the sieve-tube exudate also carried labeled proteins. 80% of the incorporated radioactivity was found in the P-protein, 20% in a neutral protein, and traces were found in acidic proteins after fractionation on ...
J, Nuske, W, Eschrich
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Filament formation in vitro of a sieve tube protein from Cucurbita maxima and Cucurbita pepo

Planta, 1975
Phloem proteins of the sieve tube exudate from Cucurbita maxima Duch. and Cucurbita pepo L. were investigated as to their filament forming ability in vitro. From the two main proteins (116000 dalton, 30000 dalton) only the 116000 dalton protein was found to form reversibly distinct filaments of 6-7 nm diameter upon removal of SH-protecting agents from ...
H, Kleinig   +3 more
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KARAKTERISTIKE BUČA Cucurbita maxima

2015
U ovom istraživanju su korišteni uzorci dviju buča Cucurbita maxima (Hokkaido i Kabocha) čiji ekstrakti su pripremani u dvije akademske godine: 2014/2015. i 2013/2014. Prošlogodišnji uzorci bili su podvrgnuti ekstrakciji uz refluksiranje i ekstrakciji u ultrazvučnoj kupelji. Fenolni spojevi svih uzoraka buča određeni su pomoću Folin-Ciocalteu metode, a
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Cucurbitacin 19-hydroxylase in Cucurbita maxima

Phytochemistry, 1978
Abstract The enzymatic hydroxylation of the C-19-methyl group of cucurbitacin B and D was observed in partly purified preparations obtained from the unripe fruit of Cucurbita maxima . Assay methods were developed and the pH optimum, cofactor requirements, and substrate specificity determined.
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Resistance in Cucurbita pepo and Cucurbita maxima germplasms to cucumber mosaic virus

Genetic Resources and Crop Evolution, 1996
A total of 384 Cucurbita pepo and Cucurbita maxima accessions were evaluated in growth chambers or glasshouse tests for resistance to cucumber mosaic virus (CMV). Seedlings were mechanically inoculated at the cotyledon stage, and scored for symptoms on cotyledons and true leaves 7 and 18 days after inoculation.
A. Lebeda, E. Křístková
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Microbial population of the laimosphere of squash (Cucurbita maxima)

Plant and Soil, 1972
The laimosphere, a term analogous to rhizosphere, describes the zone of influence of below-ground portions of shoots on soil microbial populations. Squash hypocotyls influenced microbial populations in soils 0–3 mm from hypocotyl surfaces. In this region, the laimosphere/soil ratio was 7, 36, and 7, respectively, for general bacteria, fluorescent ...
A. Magyarosy, J. G. Hancock
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Ornithine transaminase from Cucurbita maxima cotyledons

Phytochemistry, 1973
Abstract During germination a marked increase in both soluble and particulate ornthine transaminase occurs in pumpkin cotyledons. Both enzymes had a pH optimum of 8.3 and a requirement for ornthine and α-ketoglutarate. Other keto acids or amino donors showed little activity.
W.E. Splittstoesser, L. Fowden
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[Spectrophotometric analysis of phytochrome in the embryo of Cucurbita pepo and Cucurbita maxima].

Planta, 2014
Phytochrome detection and △ (△O.D.) measurements were made for the first time in a single embryo of pumpkin or gourd thanks to an apparatus adjusted to the differential spectrophotometer constructed by Dr. Spruit (Wageningen). Observed △ (△O.D.) values are comparable with usual values obtained by analysis of several seeds in a cuvette. The differential
J, Boisard, R, Malcoste
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Phloemprotein bei Cucurbita maxima

Berichte der Deutschen Botanischen Gesellschaft, 1976
ZusammenfassungDie basischen P‐proteine von Cucurbita maxima zeigen Glucansynthetase‐Aktivität. Sie werden offensichtlich ständig in den Geleitzellen neu gebildet und notwendigerweise wieder abgebaut. Ein Zusammenhang zwischen Verstopfung der Siebporen mit P‐protein‐Filamenten und Callosebildung ist zu erkennen.
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