Results 1 to 10 of about 312,868 (309)

Effect of Concentrated Fibroblast-Conditioned Media on In Vitro Maintenance of Rat Primary Hepatocyte. [PDF]

PLoS ONE, 2016
The effects of concentrated fibroblast-conditioned media were tested to determine whether hepatocyte function can be maintained without direct contact between hepatocytes and fibroblasts.
Dayeong Jeong, Chungmin Han, Inhye Kang, Hyun Taek Park, Jiyoon Kim, Hayoung Ryu, Yong Song Gho, Jaesung Park   +7 more
doaj   +4 more sources

Optimized exosome isolation protocol for cell culture supernatant and human plasma [PDF]

Journal of Extracellular Vesicles, 2015
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of ...
Richard J. Lobb, Melanie Becker, Shu Wen Wen, Christina S. F. Wong, Adrian P. Wiegmans, Antoine Leimgruber, Andreas Möller   +6 more
doaj   +6 more sources

Fibrotic effect of monoculture, conditioned media and coculture of fibroblast NIH3T3 cells on collagen secretion and mRNA TGF-β expressions [PDF]

Pharmaciana, 2023
Fibrosis is common profound in diabetes condition, they characterized by accumulation of extracellular matrix in tissue then cause tissue injury. The study aim to investigate effect of monoculture, conditioned media, and coculture fibroblast of NIH3T3 ...
Rizki Awaluddin, Dwi Aris Agung Nugrahaningsih, Eti Nurwening Solikhah, Prasetyastuti   +3 more
doaj   +1 more source

Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media

Journal of Developmental Biology, 2023
Normalizing RT-qPCR miRNA datasets that encompass numerous preimplantation embryo stages requires the identification of miRNAs that may be used as stable reference genes.
David C. Hawke, Andrew J. Watson, Dean H. Betts   +2 more
doaj   +1 more source

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics [PDF]

In Vivo, 2018
There are numerous reports on embryo culture media and conditions in the laboratory, as the subject is multifaceted and complex, reflecting the variation in practice. In this scoping review, we attempt to approach the topic of culture media and conditions from the practitioners' perspective aiming to highlight, in a comprehensive fashion, important ...
Simopoulou, M., Sfakianoudis, K., Rapani, A., Giannelou, P., Anifandis, G., Bolaris, S., Pantou, A., Lambropoulou, M., Pappas, A., Deligeoroglou, E., Pantos, K., Koutsilieris, M.   +11 more
openaire   +3 more sources

Human esophageal myofibroblasts increase squamous epithelial thickness via paracrine mechanisms in an in vitro model of gastroesophageal reflux disease

PLoS ONE, 2020
The pathogenesis of esophageal injury in gastroesophageal reflux disease (GERD) is incompletely understood. We modeled exposure of human esophageal myofibroblasts (HEMFs) to gastroesophageal reflux by repeated treatment with pH 4.5 and pH 4.5 bile salts ...
Liping Hu, Chunying Zhang, Kevin Yang, Meng Li, Anisa Shaker, Nicholas Clemons   +5 more
doaj   +3 more sources

Characterisation of human embryonic stem cells conditioning media by 1H-nuclear magnetic resonance spectroscopy. [PDF]

PLoS ONE, 2011
BACKGROUND: Cell culture media conditioned by human foreskin fibroblasts (HFFs) provide a complex supplement of protein and metabolic factors that support in vitro proliferation of human embryonic stem cells (hESCs).
David A MacIntyre, Darío Melguizo Sanchís, Beatriz Jiménez, Rubén Moreno, Miodrag Stojkovic, Antonio Pineda-Lucena   +5 more
doaj   +1 more source

Growth Factors Released from Advanced Platelet-Rich Fibrin in the Presence of Calcium-Based Silicate Materials and Their Impact on the Viability and Migration of Stem Cells of Apical Papilla

Dentistry Journal, 2023
Advanced platelet-rich fibrin (A-PRF) provides the scaffold and growth factors necessary for stem cells to proliferate and differentiate in successful regenerative endodontic procedures.
Cristine Smoczer, Kenneth R. Yuth, Mazin A. Askar, Laura A. Young, Susan B. Paurazas   +4 more
doaj   +1 more source

Laser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts

Journal of Functional Biomaterials, 2023
Titanium surface modifications are widely used to modulate cellular behavior by recognition of topographical cues. However, how those modifications affect the expression of mediators that will influence neighboring cells is still elusive.
Jorge Felipe Lima Teixeira, João Antônio Chaves de Souza, Fernando Augusto Cintra Magalhães, Guilherme José Pimentel Lopes de Oliveira, José Bernardo de Santis, Carlos Alberto de Souza Costa, Pedro Paulo Chaves de Souza   +6 more
doaj   +1 more source

Regulatory T cells provide chondroprotection through increased TIMP1, IL-10 and IL-4, but cannot mitigate the catabolic effects of IL-1β and IL-6 in a tri-culture model of osteoarthritis

Osteoarthritis and Cartilage Open, 2021
Objective: To gain insight into Treg interactions with synovial tissues in early OA, an equine tri-culture model of OA was used to test the hypothesis that Tregs, in the absence of T Helper 17 ​cells, are sufficient to resolve inflammation elicited by IL-
Laura E. Keller, Elia D. Tait Wojno, Laila Begum, Lisa A. Fortier   +3 more
doaj   +1 more source